FlyBase Allele Report: Dmel\Orai[k11505]

General Information

Symbol

Dmel\Orai^k11505

Species

D. melanogaster

Name

FlyBase ID

FBal0064167

Feature type

allele

Associated gene

Dmel\Orai

Associated Insertion(s)

P{lacW}Orai^k11505

Carried in Construct

Also Known As

dOrai¹¹⁰⁴²

Key Links

Genomic Maps

JBrowse

Allele class

hypermorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypermorphic allele - genetic evidence

(Venkiteswaran and Hasan, 2009)

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}Orai^k11505

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:17,850,913..17,850,913 [+]

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates autosomal dominant cerebellar ataxia

modeled by Itpr^ug3, Itpr^wc703

(Balasubramanian and Srinivasan, 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Phenotype Manifest In

Detailed Description

Statement

Reference

Orai^k11505 heterozygotes do not show flight defects.

(Balasubramanian and Srinivasan, 2020)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Itpr^wc703/Itpr^ug3, NUCB1^c01508, Orai^k11505 has abnormal flight phenotype, suppressible | partially by SERCA[+]/SERCA^Kum170

(Balasubramanian and Srinivasan, 2020)

Itpr^wc361/Itpr^ug3, NUCB1^c01508, Orai^k11505 has abnormal flight phenotype, suppressible | partially by SERCA[+]/SERCA^Kum170

(Balasubramanian and Srinivasan, 2020)

NOT suppressed by

Statement

Reference

Itpr^wc361/Itpr^ug3, Orai[+]/Orai^k11505, SERCA[+]/SERCA^Kum170 has abnormal flight phenotype, non-suppressible by NUCB1^c01508/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3, Orai^k11505, SERCA^Kum170 has abnormal flight phenotype, non-suppressible by NUCB1^c01508/NUCB1[+]

(Balasubramanian and Srinivasan, 2020)

Suppressor of

Statement

Reference

Orai[+], NUCB1^NIG.32190R, Orai^k11505, Scer\GAL4^Ddc.PL is a suppressor of abnormal flight phenotype of Itpr^wc703/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

NUCB1^c01508, Orai[+], Orai^k11505, NUCB1[+] is a suppressor of abnormal flight phenotype of Itpr^wc703/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

Orai[+]/Orai^k11505, NUCB1^c01508/NUCB1^c01508 is a suppressor of abnormal flight phenotype of Itpr^wc361/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

SERCA[+], Orai[+], Orai^k11505, SERCA^Kum170 is a suppressor of abnormal flight phenotype of Itpr^wc361/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

Orai[+], NUCB1^NIG.32190R, Orai^k11505, Scer\GAL4^Ddc.PL is a suppressor of abnormal flight phenotype of Itpr^wc361/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

NUCB1^c01508, Orai[+], Orai^k11505, NUCB1[+] is a suppressor of abnormal flight phenotype of Itpr^wc361/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

Orai[+]/Orai^k11505 is a suppressor of increased mortality during development | cold sensitive phenotype of Itpr^wc703/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

NUCB1^c01508, Orai[+], Orai^k11505, NUCB1[+] is a suppressor of increased mortality during development | cold sensitive phenotype of Itpr^wc703/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

Orai[+]/Orai^k11505, NUCB1^c01508/NUCB1^c01508 is a suppressor of abnormal flight phenotype of Itpr^wc703/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

SERCA[+], Orai[+], Orai^k11505, SERCA^Kum170 is a suppressor of abnormal flight phenotype of Itpr^wc703/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

NOT Suppressor of

Statement

Reference

Orai[+]/Orai^k11505 is a non-suppressor of abnormal flight phenotype of Itpr^wc703/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

Orai[+]/Orai^k11505 is a non-suppressor of abnormal flight phenotype of Itpr^wc361/Itpr^ug3

(Balasubramanian and Srinivasan, 2020)

Other

Statement

Reference

Itpr^wc361/Itpr^ug3, NUCB1^c01508, Orai^k11505 has abnormal flight phenotype

(Balasubramanian and Srinivasan, 2020)

Itpr^wc703/Itpr^ug3, NUCB1^c01508, Orai^k11505 has abnormal flight phenotype

(Balasubramanian and Srinivasan, 2020)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

SERCA^Kum170, Orai^k11505 double heterozygotes do not show flight defects.

(Balasubramanian and Srinivasan, 2020)

Introduction of a single copy of olf186-F^k11505 suppresses the cold-sensitive lethality found in Itp-r83A^ka1091/Itp-r83A^ug3 mutants at 17.5[o]C. The wing posture defect found in these mutants when grown at 25[o]C is also suppressed to a significant extent.

The presence of a single copy of olf186-F^k11505 in Itp-r83A^ka1091/Itp-r83A^ug3 mutants restores flight initiation in response to an air puff and suppresses hyperactivity of flight neuromuscular junctions.

Ca-P60A^Kum170/olf186-F^k11505; Itp-r83A^ka1091/Itp-r83A^ug3 flies exhibit normal wings and normal levels of spontaneous electrical activity in DLM recordings. Flight ability is restored in a significant number of these triple mutant flies. This is in contrast to the complete loss of flight ability in Itp-r83A^ka1091/Itp-r83A^ug3 mutants and in olf186-F^k11505/+; Itp-r83A^ka1091/Itp-r83A^ug3 mutants. Over 60% of olf186-F^k11505/Ca-P60A^Kum170; Itp-r83A^ka1091/Itp-r83A^ug3 adults pass as 'fliers' in the cylinder drop test assay. Air-puff delivery elicited sustainable rhythmic flight patterns similar to wild-type in a high proportion of these flies.

(Venkiteswaran and Hasan, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

111309

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}Orai^k11505 /CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Complements: thr^k07805b.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

Orai^k11505

dOrai¹¹⁰⁴²

(Balasubramanian and Srinivasan, 2020, Venkiteswaran and Hasan, 2009)

dOrai¹

(Venkiteswaran and Hasan, 2009)

l(2)k11505

(Spradling et al., 1999)

l(2)k11505^k11505

olf186-F¹

(Venkiteswaran and Hasan, 2009)

olf186-F^k11505

orai^k11505

(Suisse and Treisman, 2019)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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