FlyBase Allele Report: Dmel\tsr[k05633]

General Information

Symbol

Dmel\tsr^k05633

Species

D. melanogaster

Name

FlyBase ID

FBal0064474

Feature type

allele

Associated gene

Dmel\tsr

Associated Insertion(s)

P{lacW}tsr^k05633

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}tsr^k05633

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:24,045,156..24,045,156 [+]

(Janody and Treisman, 2006, Chandrasekaran and Beckendorf, 2005, Leyssen et al., 2005, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA with RhoGAP18B¹

(Ojelade et al., 2015)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(BDGP Project Members, 1994-1999)

Phenotype Manifest In

actin cytoskeleton & eye disc | somatic clone

(Delalle et al., 2005)

eye | somatic clone

(Delalle et al., 2005)

ommatidium | somatic clone

(Delalle et al., 2005)

rhabdomere | somatic clone

(Delalle et al., 2005)

Detailed Description

Statement

Reference

tsr^k05633 embryos show normal salivary glands.

(Chandrasekaran and Beckendorf, 2005)

Homozygous clones in the eye are small compared to their wild-type sister clones and the mutant clones sometimes contain black dots, indicating cell death. The mutant clones show a loss of ommatidial architecture and have misshapen rhabdomeres. Mutant clones in third larval instar eye discs accumulate actin.

(Delalle et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

tsr[+]/tsr^k05633 is a non-suppressor of abnormal neuroanatomy phenotype of Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf

(Leyssen et al., 2005)

Other

Statement

Reference

RhoGAP18B¹, tsr[+]/tsr^k05633 has chemical resistant | adult stage phenotype

(Ojelade et al., 2015)

tsr^k05633, twf^EP3701 has visible phenotype

(Wahlstrom et al., 2001)

Phenotype Manifest In

Enhancer of

Statement

Reference

tsr^k05633 is an enhancer of ommatidium phenotype of twf^EP3701

(Wahlstrom et al., 2001)

Suppressor of

Statement

Reference

tsr[+]/tsr^k05633 is a suppressor of eye phenotype of mbt^P3

(Menzel et al., 2007)

tsr^k05633 is a suppressor of actin cytoskeleton phenotype of Btk^k00206

(Chandrasekaran and Beckendorf, 2005)

tsr^k05633 is a suppressor of presumptive embryonic salivary gland phenotype of Btk^k00206

(Chandrasekaran and Beckendorf, 2005)

tsr[+]/tsr^k05633 is a suppressor of wing phenotype of Pax^UAS.cCa, Scer\GAL4^32B

(Chen et al., 2005)

NOT Suppressor of

Statement

Reference

tsr[+]/tsr^k05633 is a non-suppressor of ventral adult lateral neuron & axon phenotype of Hsap\APP^UAS.Tag:MYC, Scer\GAL4^P2.4.Pdf

(Leyssen et al., 2005)

Other

Statement

Reference

tsr^k05633, twf^EP3701 has macrochaeta phenotype

(Wahlstrom et al., 2001)

tsr^k05633, twf^EP3701 has anterior dorsocentral bristle phenotype

(Wahlstrom et al., 2001)

tsr^k05633, twf^EP3701 has posterior scutellar bristle phenotype

(Wahlstrom et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The increased resistance to ethanol-induced sedation observed in RhoGAP18B¹/RhoGAP18B¹ adults cannot be suppressed by combination with tsr^k05633 in heterozygous state but RhoGAP18B¹/+;tsr^k05633/+ double heterozygotes display significantly higher resistance than RhoGAP18B¹/+ single heterozygotes.

(Ojelade et al., 2015)

The rough eye phenotype of mbt^P3 hemizygous males is suppressed by tsr^k05633/+.

(Menzel et al., 2007)

The actin disorganization and salivary gland phenotypes of Btk29A^k00206 single mutants are rescued in Btk29A^k00206; tsr^k05633 double mutants.

(Chandrasekaran and Beckendorf, 2005)

The blistered wing phenotype observed in flies expressing Pax^Scer\UAS.cCa under the control of Scer\GAL4^32B is dominantly suppressed in a tsr^k05633/+ background.

(Chen et al., 2005)

Nearly all twf^EP3701 tsr^k05633 double heterozygotes have at least one macrochaeta with defects at the bristle tip. The posterior scutellar and anterior dorsocentral bristles are the most frequently affected. 66% of bristles are split, branched or have a rough surface in these animals. tsr^k05633/+ enhances the eye defects seen in twf^EP3701 homozygotes.

(Wahlstrom et al., 2001)

Xenogenetic Interactions

Statement

Reference

Heterozygosity for tsr^k05633 does not interfere with the Scer\GAL4^P2.4.Pdf>Hsap\APP^{Scer\UAS.T:Hsap\MYC}-induced increase in sLNv axonal arborization.

(Leyssen et al., 2005)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

12201

y¹ w^67c23; P{lacW}tsr^k05633/CyO

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Complements: eIF-5A⁰¹²⁹⁶. Complements: Adk2-60B^k04201. Complements: Chi^k04405. Complements: Phm^k07623.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

ADFcofilin^k05633

(Silver and Montell, 2001)

l(2)k05633^k05633

(Janody and Treisman, 2006, BDGP Project Members, 1994-1999)

tsr^k05633

(Skouloudaki et al., 2019, Ojelade et al., 2015, Menzel et al., 2007, Chen et al., 2005)

tsr^k05

(Ojelade et al., 2015)

tsr^ko5633

(Delalle et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

Open Close