FlyBase Allele Report: Dmel\Hrb27C[k02814]

General Information

Symbol

Dmel\Hrb27C^k02814

Species

D. melanogaster

Name

FlyBase ID

FBal0064757

Feature type

allele

Associated gene

Dmel\Hrb27C

Associated Insertion(s)

P{lacW}Hrb27C^k02814

Carried in Construct

Also Known As

hrp48^k02814

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}Hrb27C^k02814

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage | somatic clone

(Bruckert et al., 2015)

lethal

(Bruckert et al., 2015, Goodrich et al., 2004)

lethal (with Df(2L)wee^wo5)

(Yano et al., 2004)

lethal (with Hrb27C^5A2-6)

(Huynh et al., 2004)

lethal (with Hrb27C^7E7-18)

(Huynh et al., 2004)

lethal (with Hrb27C^10B2-9)

(Huynh et al., 2004)

lethal (with Hrb27C^k10413)

(Yano et al., 2004)

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Phenotype Manifest In

dorsal appendage | germline clone

(Goodrich et al., 2004)

egg | germline clone

(Goodrich et al., 2004)

egg chorion | germline clone

(Goodrich et al., 2004)

egg operculum | germline clone

(Goodrich et al., 2004)

lobe system of adult mushroom body | somatic clone

(Bruckert et al., 2015)

oocyte & cytoskeleton | germ-line clone

(Yano et al., 2004)

ovary | germline clone

(Yano et al., 2004)

Detailed Description

Statement

Reference

Mushroom Body neuroblast clones composed of neurons homozygous for Hrb27C^k02814 exhibit ectopic extension of dorsal bundles. The proportion of MBs with axon overextension defects is higher in females than in males.

(Bruckert et al., 2015)

The eggs laid by Hrb27C^k02814 germ-line clone carrying females exhibit varying degrees of dorsalisation: least affect have widely spaced dorsal appendage and an enlarged operculum; intermediately affected have a single broad appendage that spans at least the width of the normal appendages; severely affected have a crown of appendage material that surrounds the anterior circumference of the egg. Most of the eggs laid are abnormally short.

(Goodrich et al., 2004)

The majority of Hrb27C^k02814 germline clones fail to develop beyond the early stages of oogenesis. The organisation and polarity of the oocyte cytoskeleton is somewhat abberant in these germline clones.

(Yano et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

Hrb27C[+]/Hrb27C^k02814 is an enhancer of embryonic/first instar larval cuticle & embryonic head phenotype of Scer\GAL4^{VP16.nanos.UTR}, osk^3'UTR.UASp

(Yano et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

The penetrance and severity of embryonic cuticle patterning defects in osk^{3'UTR.Scer\UAS.P\T}; Scer\GAL4^{nos.UTR.T:Hsim\VP16} embryos is enhanced by Hrb27C^k02814/+.

(Yano et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Hrb27C^7E7-18

(Huynh et al., 2004)

Hrb27C^10B2-9

(Huynh et al., 2004)

Hrb27C^5A2-6

(Huynh et al., 2004)

Hrb27C⁰²⁶⁴⁷

(BDGP Project Members, 1994-1999)

Partially rescued by

Hrb27C^k02814 is partially rescued by Scer\GAL4^ey-OK107/Hrb27C^UAS.Tag:FLAG

(Bruckert et al., 2015)

Comments

Expression of Hrb27C^UAS.Tag:FLAG under the control of Scer\GAL4^ey-OK107 partially rescues ectopic extension of dorsal bundles of Mushroom Body neuroblast clones composed of neurons homozygous for Hrb27C^k02814.

(Bruckert et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

10375

y¹ w^67c23; P{lacW}Hrb27C^k02814/CyO

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Allelic series: Hrb27C^k02814 > Hrb27C^k16203 > Hrb27C⁰²⁶⁴⁷

(Yano et al., 2004)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

BL-10375

(Rollmann et al., 2007)

Hrb27C^k02814

Hrp48^K02814

(Mhlanga et al., 2009)

hrp48^K02814

(Bruckert et al., 2015)

hrp48^k02814

(Yano et al., 2004)

l(2)02647^k02814

l(2)k02814

(Spradling et al., 1999)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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