FlyBase Allele Report: Dmel\Hrb27C[02647]

General Information

Symbol

Dmel\Hrb27C⁰²⁶⁴⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0008005

Feature type

allele

Associated gene

Dmel\Hrb27C

Associated Insertion(s)

P{PZ}Hrb27C⁰²⁶⁴⁷

Carried in Construct

Also Known As

l(2)02647, hrp48⁰²⁶⁴⁷

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Rottgen et al., 1998, Campbell et al., 1995)

Progenitor genotype

Associated Insertion(s)

P{PZ}Hrb27C⁰²⁶⁴⁷

Cytology

Description

P{PZ} insertion 3.3kb from the Hrb27C coding sequence.

(Hammond et al., 1997)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:6,920,714..6,920,714 [+]

(Mhlanga et al., 2009, Sofola et al., 2007, Deal and Cook, 2005.7.11, Goodrich et al., 2004, Huynh et al., 2004, Yano et al., 2004, Price et al., 2000, Vorbruggen et al., 2000, Vorbruggen et al., 2000, Spradling et al., 1999, Rottgen et al., 1998, Hammond et al., 1997, Campbell et al., 1995, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage (with Hrb27C^k10413)

(Bruckert et al., 2015)

lethal

(Goodrich et al., 2004)

lethal (with Df(2L)wee^wo5)

(Yano et al., 2004)

lethal (with Hrb27C^5A2-6)

(Huynh et al., 2004)

lethal (with Hrb27C^7E7-18)

(Huynh et al., 2004)

lethal (with Hrb27C^10B2-9)

(Huynh et al., 2004)

lethal (with Hrb27C^EL2)

(Price et al., 2000)

lethal | recessive

(Price et al., 2000, Spradling et al., 1999)

lethal - all die before end of larval stage | recessive

(Hammond et al., 1997)

lethal - all die during embryonic stage | recessive

(Campbell et al., 1995)

partially lethal (with Hrb27C^k10413)

(Bruckert et al., 2015)

partially lethal - majority die (with Hrb27C^k10413)

(Yano et al., 2004)

some die during embryonic stage | recessive

(Campbell et al., 1995)

some die during larval stage | recessive

(Hammond et al., 1997)

viable (with Hrb27C^k10413)

(Price et al., 2000)

visible (with Hrb27C^k10413)

(Price et al., 2000)

Phenotype Manifest In

egg (with Hrb27C³⁷⁷)

(Goodrich et al., 2004)

egg chorion (with Hrb27C³⁷⁷)

(Goodrich et al., 2004)

lobe system of adult mushroom body (with Hrb27C^k10413)

(Bruckert et al., 2015)

nurse cell & nuclear chromosome (with Hrb27C³⁷⁷)

(Goodrich et al., 2004)

oocyte & cytoskeleton | germ-line clone

(Yano et al., 2004)

wing margin (with Hrb27C^k10413)

(Price et al., 2000)

Detailed Description

Statement

Reference

Hrb27C^k10413/Hrb27C⁰²⁶⁴⁷ transheterozygous flies frequently have Mushroom Bodies (MB) with α and β lobes of unequal width (asymmetric), truncated α lobes or MBs lacking either the α or the β lobes when compared to controls. They also exhibit abnormal overextended αβ dorsal branches that systematically grow toward the midline when compared to controls.

(Bruckert et al., 2015)

<2% of eggs laid by Hrb27C⁰²⁶⁴⁷/Hrb27C³⁷⁷ females exhibit any degree of dorsalisation. Nurse cell chromosomes in Hrb27C³⁷⁷/Hrb27C⁰²⁶⁴⁷ females do not disperse properly after stage 6 of oogenesis.

(Goodrich et al., 2004)

The organisation and polarity of the oocyte cytoskeleton is somewhat abberant in these Hrb27C⁰²⁶⁴⁷/Hrb27C⁰²⁶⁴⁷ germline clones.

(Yano et al., 2004)

Hrb27C^k10413/Hrb27C⁰²⁶⁴⁷ flies are viable but usually have small nicks in the wing margin.

(Price et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Hrb27C⁰²⁶⁴⁷ is an enhancer of visible phenotype of Scer\GAL4^455.2, l(1)sc^UAS.cHa

(Rottgen et al., 1998)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hrb27C³⁷⁷/Hrb27C⁰²⁶⁴⁷ has nurse cell & nuclear chromosome phenotype, suppressible | partially by otu¹⁰⁴

(Goodrich et al., 2004)

Enhancer of

Statement

Reference

Hrb27C³⁷⁷/Hrb27C⁰²⁶⁴⁷ is an enhancer of egg chorion phenotype of sqd¹/sqd^K12

(Goodrich et al., 2004)

Hrb27C³⁷⁷/Hrb27C⁰²⁶⁴⁷ is an enhancer of dorsal appendage phenotype of sqd¹/sqd^K12

(Goodrich et al., 2004)

Hrb27C³⁷⁷/Hrb27C⁰²⁶⁴⁷ is an enhancer of egg operculum phenotype of sqd¹/sqd^K12

(Goodrich et al., 2004)

Hrb27C³⁷⁷/Hrb27C⁰²⁶⁴⁷ is an enhancer of egg phenotype of sqd¹/sqd^K12

(Goodrich et al., 2004)

Hrb27C[+]/Hrb27C⁰²⁶⁴⁷ is an enhancer of embryonic/first instar larval cuticle & embryonic head phenotype of Scer\GAL4^{VP16.nanos.UTR}, osk^3'UTR.UASp

(Yano et al., 2004)

Hrb27C⁰²⁶⁴⁷ is an enhancer of scutellar bristle phenotype of Scer\GAL4^455.2, l(1)sc^UAS.cHa

(Rottgen et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Dorsalisation of the eggs laid by sqd¹/sqd^K12 transheterozygotes is enhanced by Hrb27C⁰²⁶⁴⁷/Hrb27C³⁷⁷ so that 100% show some degree of dorsalisation.

(Goodrich et al., 2004)

The penetrance and severity of embryonic cuticle patterning defects in osk^{3'UTR.Scer\UAS.P\T}; Scer\GAL4^{nos.UTR.T:Hsim\VP16} embryos is enhanced by Hrb27C⁰²⁶⁴⁷/+.

(Yano et al., 2004)

Mutation enhances the ectopic scutellar bristle phenotype seen from Scer\GAL4^455.2-mediated expression of l(1)sc^Scer\UAS.cHa.

(Rottgen et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Hrb27C^7E7-18

(Huynh et al., 2004)