Homozygous Chig96.1 embryos laid by Chig96.1/+ mothers die before reaching the larval stage. Heterozygous Chig96.1 embryos laid by Chig96.1/+ mothers segment normally and survive to adulthood.
The projections of tup-expressing intersegmental nerve motor neurons show no clear alterations compared to wild type in homozygous embryos.