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General Information
Symbol
Dmel\gcmUAS.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Bernardoni
FlyBase ID
FBal0082911
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-gcm, UAS-glide, UAS-glide/gcm
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Full length gcm cDNA is expressed under the control of UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

mesothoracic tergum & macrochaeta | supernumerary | conditional ts, with Scer\GAL4hs.PB

Detailed Description
Statement
Reference

The expression of gcmScer\UAS.cBa under the control of Scer\GAL4gcm-rA87.P, the intrinsic Gal4 from the gcmrA87.P allele, leads to morphological defects of the spermathecum, which displays a highly significant increase in the number of secretory cells, a significant decrease in the number of basal cells, but no obvious defects in the lumen epithelial cells and in the inner cuticular structure, as compared to controls; expression under the control of Scer\GAL4lz-gal4 leads to severe morphological defects of the spermathecum, including a deformed or almost absent inner cuticular structure, as compared to controls.

Mis-expression of gcmScer\UAS.cBa under the control of either Scer\GAL4elav-C155 or Scer\GAL4αTub84B.PL results in a marked increase in the number of glial cells in type II neuroblast clones. Similar results are obtained by driving mis-expression of gcmScer\UAS.cBa with Scer\GAL4erm.R9D11.

Expression of gcmScer\UAS.cBa under the control of Scer\GAL4gcm-rA87.P results in an increased glial cell population in the embryo. Increased glial cell proliferation is observed in these animals.

Expression of gcmScer\UAS.cBa throughout the embryonic CNS, driven by Scer\GAL4sca-4512, induces the formation of additional subperineural glia.

Expression of gcmScer\UAS.cBa under the control of Scer\GAL4hs.PB using heat shock during the third larval instar stage results in lethality. Expression of gcmScer\UAS.cBa under the control of Scer\GAL4hs.PB during the white pupa stage using heat shock results in extra bristles on the thorax wing. Expression of gcmScer\UAS.cBa under the control of Scer\GAL4hs.PB during the pupal stage using heat shock does not result in extra bristles on the thorax or wing.

Expression of gcmScer\UAS.cBa, when driven by Scer\GAL4eg-Mz360 or Scer\GAL4sca-4512 induces ectopic NB6-4T like glial cells, though they are located at more lateral positions than in wild-type.

When expression is driven by Scer\GAL4lz-gal4 crystal cells are transformed into plasmatocytes.

Expression of gcmScer\UAS.cBa under the control of Scer\GAL4hs.PB using heat shock at around 0 hours after puparium formation (APF) produces no effects on development of the L3-3 sensory organ. Expression using heat shock at 2 hours APF results in the production of an "L3-3" sensory organ consisting of 4 cells (at the corresponding stage in wild-type animals, 5 cells are seen). The 2 distal cells correspond to the tormogen and trichogen cells. The 2 proximal cells express the repo glial-specific marker. Expression using heat shock at 5 hours APF results in the production of an "L3-3" sensory organ consisting of 5 cells, 3 of which express the repo glial-specific marker. The other 2 cells correspond to the tormogen and trichogen cells.

Expression of gcmScer\UAS.cBa by Scer\GAL4sca-T3 induces gliogenesis by repressing the neuroblast fate.

When expression is driven by Scer\GAL4twi.PB, muscle fibers are reduced in number and poorly organized. Embryos display a significant number of round, unfused muscle cells. Mesodermal cells are transformed to a glial cell fate. Visceral mesoderm shows abnormalities reflected in disorganization of gut cells. Overall ventral nerve cord morphology is abnormal, midline cannot be recognized; posterior and anterior commissures may be fused or absent. When expression is driven by Scer\GAL4pnr-MD237, dorsal closure fails resulting in dorsally herniated embryos. Some cells show evidence of glial cell identity.

Embryos expressing gcmScer\UAS.cBa under the control of Scer\GAL4elav.PLu produce ectopic hemocytes.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of gcmScer\UAS.cBa and hkbScer\UAS.cMa in the embryonic CNS, driven by Scer\GAL4sca-4512, induces greater levels of additional subperineural glia than the expression of either transgene alone.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The expression of gcmScer\UAS.cBa under the control of Scer\GAL4gcm-rA87.P, the intrinsic Gal4 from the gcmrA87.P allele, rescues the absence of secretory cells observed in gcmrA87.P homozygous spermathecae; this expression also rescues the decreased number of secretory cells in spermathecae expressing gcmJF01075 under the control of Scer\GAL4gcm-rA87.P, even leading to their significant increase in number as compared to controls.

Stage 17 gcmScer\UAS.cBa, Scer\GAL4sr-md710; Df(2L)132/Df(2L)132 embryos do not have the muscle attachment defects seen in Df(2L)132/Df(2L)132 or gcmN7-4/Df(2L)132 embryos.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
gcmScer\UAS.cBa
gcmUAS.cBa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Bernardoni
Secondary FlyBase IDs
    References (18)