FlyBase Allele Report: Dmel\heca[UAS.cSa]

General Information

Symbol

Dmel\heca^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Steneberg

FlyBase ID

FBal0089237

Feature type

allele

Associated gene

Dmel\heca

Associated Insertion(s)

Carried in Construct

P{UAS-heca.S}

Also Known As

UAS-hdc

Key Links

Transgenic product class

cDNA

(Steneberg et al., 1998)

wild_type

(Steneberg et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Steneberg et al., 1998)

wild_type

(Steneberg et al., 1998)

Mutagen

in vitro construct

(Steneberg et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-heca.S}

Cytology

Description

UASt regulatory sequences drive expression of a 3.7kb KpnI-NotI cDNA fragment corresponding to the full-length hdc mRNA that contains two UAA stop codons (translational readthrough of the first, in-frame, stop codon can occur, thus this construct can produce two protein isoforms).

(Steneberg et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

heca

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval tracheal section, with Scer\GAL4^btl.PS

(Steneberg et al., 1998)

embryonic/larval tracheal section, with Scer\GAL4^esg-p127

(Steneberg et al., 1998)

Detailed Description

Statement

Reference

Overexpression of hdc^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PU does not affect the differentiation of photoreceptors R1/6.

(Avet-Rochex et al., 2014)

Expression of hdc^Scer\UAS.cSa under the control of Scer\GAL4^ppk.1.9 has no effect on the development of the proximal dendrites of the ddaC dorsal dendritic arborising neurons and no degeneration or cell death is seen in these neurons during the larval stages. Pruning of the ddaC dendrites does not appear significantly different from wild type.

(Loncle and Williams, 2012)

Scer\GAL4^btl.PS-mediated expression in wild type embryos suppresses terminal branching of the tracheal system. In a few embryos Scer\GAL4^btl.PS-mediated and Scer\GAL4^p127-mediated expression also causes extra branching phenotypes.

(Steneberg et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

heca^UAS.cSa, Scer\GAL4^ppk.1.9 is a suppressor | partially of abnormal neuroanatomy phenotype of EcR^RNAi.UAS.cCa, Scer\GAL4^ppk.1.9

(Loncle and Williams, 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

unk^UAS.cARa, heca^UAS.cSa, Scer\GAL4^GMR.PU is a suppressor of photoreceptor cell R7 | somatic clone phenotype of Tsc1^Q600X

(Avet-Rochex et al., 2014)

unk^UAS.cARa, heca^UAS.cSa, Scer\GAL4^GMR.PU is a suppressor of cone cell | somatic clone phenotype of Tsc1^Q600X

(Avet-Rochex et al., 2014)

heca^UAS.cSa, Scer\GAL4^ppk.1.9 is a suppressor | partially of larval dorsal multidendritic neuron ddaC phenotype of EcR^RNAi.UAS.cCa, Scer\GAL4^ppk.1.9

(Loncle and Williams, 2012)

heca^UAS.cSa, Scer\GAL4^ppk.1.9 is a suppressor | partially of dendrite phenotype of EcR^RNAi.UAS.cCa, Scer\GAL4^ppk.1.9

(Loncle and Williams, 2012)

Other

Statement

Reference

Scer\GAL4^GMR.PU, heca^UAS.cSa, unk^UAS.cARa has eye phenotype

(Avet-Rochex et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of hdc^Scer\UAS.cSa and unk^{Scer\UAS.cARa} under the control of Scer\GAL4^GMR.PU affects eye development.

Overexpression of unk^{Scer\UAS.cARa} under the control of Scer\GAL4^GMR.PU in Tsc1^Q600X mutant eye clones does not affect the precocious differentiation of R7 photoreceptor cells and cone cells.

Co-expression of hdc^Scer\UAS.cSa and unk^{Scer\UAS.cARa} under the control of Scer\GAL4^GMR.PU in Tsc1^Q600X mutant eye clones suppresses the precocious differentiation of R7 and cone cells. Neither the delay in differentiation, nor the rough eye phenotype are due to increased apoptosis.

(Avet-Rochex et al., 2014)

Co-expression of hdc^Scer\UAS.cSa does not suppress the dendrite severing defects seen in the ddaC dorsal dendritic arborising neurons of animals expressing Sox14^GD4416 under the control of Scer\GAL4^ppk.1.9 at 18 hours after puparium formation.

Co-expression of hdc^Scer\UAS.cSa partially suppresses the dendrite severing defects seen in the ddaC dorsal dendritic arborising neurons of animals expressing EcR^{dsRNA.Scer\UAS.cCa} under the control of Scer\GAL4^ppk.1.9 at 18 hours after puparium formation.

(Loncle and Williams, 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^heca-19/heca^UAS.cSa rescues heca¹⁹

(Varga et al., 2019)

Scer\GAL4^{kn-col85-GAL4}/heca^UAS.cSa rescues heca⁴³

(Varga et al., 2019)

heca^UAS.cSa/Scer\GAL4^ppk.1.9 rescues heca⁴³

(Loncle and Williams, 2012)

Scer\GAL4^esg-p127/heca^UAS.cSa rescues heca^Fus-6-50

(Steneberg et al., 1998)

Comments

Expression of hdc^Scer\UAS.cSa under the control of Scer\GAL4^ppk.1.9 rescues the defects in dendrite severing which are seen in the ddaC neurons of hdc⁴³ homozygous pupae.

(Loncle and Williams, 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

64065

w^*; P{UAS-heca.S}3, P{neoFRT}82B/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

hdc^Scer\UAS.cSa

hdc^UAS.cSa

heca^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Steneberg

(Steneberg et al., 1998)

Secondary FlyBase IDs

References (6)

Report Sections

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