FlyBase Allele Report: Dmel\cnn[mfs3]

General Information

Symbol

Dmel\cnn^mfs3

Species

D. melanogaster

Name

FlyBase ID

FBal0089468

Feature type

allele

Associated gene

Dmel\cnn

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Megraw et al., 1999, Li et al., 1998)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q959term.

(Megraw et al., 1999)

The premature stop codon falls within the third leucine zipper and is predicted to result in a 190 amino acid truncation of the protein.

(Megraw et al., 1999)

Nucleotide substitution: C2875T.

(Megraw et al., 1999)

Stop codon upstream of the normal translation termination signal.

(Li et al., 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:13,440,707..13,440,707 [-]

Nucleotide change:

C13440707T

Reported nucleotide change:

C2875T.

Amino acid change:

Reported amino acid change:

Q959term

(Megraw et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis | male (with Df(2R)cnn)

(Li et al., 1998)

abnormal mitotic cell cycle

(Eisman et al., 2009)

female sterile

(Li et al., 1998)

female sterile | recessive

(Megraw et al., 1999)

lethal (with Df(2R)BSC306)

(Kahsai and Cook, 2018)

lethal - all die during embryonic stage

(Eisman et al., 2009)

lethal - all die during embryonic stage | maternal effect | recessive

(Megraw et al., 1999)

male sterile

(Li et al., 1998)

male sterile | recessive

(Megraw et al., 1999)

partially lethal - majority die | maternal effect, with Scer\GAL4^{VP16.nanos.UTR}, cnn^PA.UASp.GFP

(Eisman et al., 2015)

partially lethal - majority die | maternal effect, with Scer\GAL4^{VP16.nanos.UTR}, cnn^{S22A.PA.UASp.EGFP}

(Eisman et al., 2015)

partially lethal - majority die | maternal effect, with Scer\GAL4^{VP16.nanos.UTR}, cnn^{S22A.T27A.PA.UASp.EGFP}

(Eisman et al., 2015)

partially lethal - majority die | maternal effect, with Scer\GAL4^{VP16.nanos.UTR}, cnn^{T27A.PA.UASp.EGFP}

(Eisman et al., 2015)

some die during embryonic stage

(Eisman et al., 2009)

some die during embryonic stage | recessive | maternal effect | recessive

(Megraw et al., 1999)

viable

(Megraw et al., 1999)

Phenotype Manifest In

cyst progenitor cell (with cnn^HK21)

(Cheng et al., 2011)

embryo | maternal effect

(Megraw et al., 1999)

male germline stem cell | increased number (with cnn^HK21)

(Yamashita et al., 2003)

meiotic cell cycle | male

(Li et al., 1998)

nucleus | embryonic stage | maternal effect

(Megraw et al., 1999)

nucleus | embryonic stage | maternal effect (with Df(2R)cnn), with cnn^ΔEx1A

(Eisman et al., 2015)

spermatid

(Li et al., 1998)

spindle

(Eisman et al., 2009)

spindle (with cnn^HK21)

(Cheng et al., 2011)

spindle | embryonic stage | maternal effect (with Df(2R)cnn), with cnn^ΔEx1A

(Eisman et al., 2015)

Detailed Description

Statement

Reference

cnn^mfs3/Df(2R)BSC306 results in lethality with escapers.

(Kahsai and Cook, 2018)

Embryos produced by cnn^mfs3/Df(2R)cnn; cnn^ΔEx1A females exhibit multiple nuclei and spindles scattered throughout the embryo (deep and cortical) and frequent nuclear fusions. These embryos produce normal triploid polar bodies following the completion of meiosis. In the mutant embryos, early spindles frequently have an obvious spindle pole, but the poles are lost as development proceeds and barrel-shaped anastral spindles accumulate, accompanied by nuclear fusions.

The number of adults that develop to adulthood represents only 32% of eggs laid by cnn^mfs3 mutant females that also express cnn^{PA.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

The number of adults that develop to adulthood represents only 1% of eggs laid by cnn^mfs3 mutant females that also express cnn^{S22A.PA.Scer\UAS.P\T.T:Avic\EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

The number of adults that develop to adulthood represents only 1% of eggs laid by cnn^mfs3 mutant females that also express cnn^{T27A.PA.Scer\UAS.P\T.T:Avic\EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

The number of adults that develop to adulthood represents only 14% of eggs laid by cnn^mfs3 mutant females that also express cnn^{S22A.T27A.PA.Scer\UAS.P\T.T:Avic\EGFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Eisman et al., 2015)

Only 37% of testis somatic cyst stem cells show repositioning of the mitotic spindle during anaphase in cnn^mfs3/cnn^HK21 males compared to 78% of cases in heterozygous controls.

(Cheng et al., 2011)

The failure of embryogenesis in mutant animals is associated with the appearance of multiple metaphase spindles fused at a common pole.

(Eisman et al., 2009)

The number of germ cells associated with the hub is increased 20-30% in homozygous males, from an average of 8.94 GSCs per hub in wild type to 10.69 GSCs per hub in cnn^HK21/cnn^mfs3 males. In some cases in cnn^HK21/cnn^mfs3 testes, a GSC divides and the two daughter cells remain in contact with the hub (this is not observed in the wild type).

(Yamashita et al., 2003)

Homozygous females produce embryos that arrest prior to cellularisation. Embryos derived from cnn^mfs3/Df(2R)cnn females have severe defects in nuclear division and distribution. The embryos never achieve cellularisation.

(Megraw et al., 1999)

Cytokinesis and karyokinesis during meiotic divisions are disrupted in males. The percentage of aberrant spermatids varies from testis to testis (20-50%), and the cytokinetic defects are more severe than karyokinetic defects.

(Li et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

cnn^HK21

(Megraw et al., 1999)

Rescued by

cnn^mfs3 is rescued by Scer\GAL4^{VP16.nanos.UTR}/cnn^PA.UASp.GFP

(Eisman et al., 2009)

cnn^mfs3 is rescued by cnn^+t16

(Megraw et al., 1999)

cnn^mfs3 is rescued by cnn^+t16

(Li et al., 1998)

Partially rescued by

cnn^mfs3 is partially rescued by cnn^hs.PL

(Li et al., 1998)

Comments

Expression of cnn^{PA.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues embryogenesis in cnn^mfs3 animals; approximately 92% of eggs produced by cnn^mfs3 females expressing cnn^{PA.Scer\UAS.P\T.T:Avic\GFP} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} hatch.

(Eisman et al., 2009)

Male and female sterility is rescued by cnn^+t16. Male sterility is rescued by basal levels of expression (at 25^oC) of cnn^hs.PL.

(Li et al., 1998)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

27626

w¹¹¹⁸; cnn^mfs3/SM5

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Cnn^mfs3

(Eisman et al., 2015)

arr^mfs3

cnn^mfs3

(Jones et al., 2024, Kahsai and Cook, 2018, Chen et al., 2017, Eisman et al., 2015, Cheng et al., 2011, Eisman et al., 2009)

mfs3

(Li et al., 1998)

Name Synonyms

Secondary FlyBase IDs

References (9)

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