FB2020_06, released Dec 22, 2020

Allele: Hsap\ATXN3tr.Q27.UAS.Tag:HA

Open Close
General Information
Symbol
Hsap\ATXN3tr.Q27.UAS.Tag:HA
Species
H. sapiens
Name
FlyBase ID
FBal0090631
Feature type
allele
Associated gene
Hsap\ATXN3
Associated Insertion(s)
Carried in Construct
P{UAS-hATXN3.tr-Q27}
Also Known As
UAS-MJDtr-Q27, UAS-SCA3tr-Q27, UAS-MJDtrQ27, UAS-SCA3trQ27
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Warrick et al., 1998)
in vitro construct - coding region fusion
(Warrick et al., 1998)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{UAS-hATXN3.tr-Q27}
      Cytology
      Nature of the lesion
      Statement
      Reference

      A truncated form of Hsap\ATXN3, consisting of the normal length glutamine tract (27 repeats) flanked upstream by 12 amino acids and downstream by the carboxy terminal 43 amino acids of Hsap\ATXN3, is expressed under the control of UASt regulatory sequences. The Hsap\ATXN3 protein is tagged at the N-terminal end with Tag:HA.

      (Warrick et al., 1998)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UASt
      Encoded product / tool
      Hsap\ATXN3
      Tagged with
      Tag:HA
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  neurodegenerative disease
      CEA
      (Kretzschmar et al., 2005)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Adults expressing Hsap\ATXN3tr.Q27.UAS.Tag:HA under the control of Scer\GAL4GMR.PU do not present any major eye defects.

      (Chen et al., 2019)

      Flies expressing Hsap\ATXN3tr.Q27.Scer\UAS.T:Ivir\HA1 using any one of the drivers Scer\GAL4GMR.PU, Scer\GAL4da.PU, or Scer\GAL4nrv2.PS appear morphologically and behaviorally normal.

      (Lin et al., 2015)

      The addition of 0-10mM LiCl to 1 day old Scer\GAL4nrv2.PS>Hsap\ATXN3tr.Q27.Scer\UAS.T:Ivir\HA1 does not generate a remarkable difference in the locomotor ability compared to non-treated controls.

      (Jia et al., 2013)

      Larval ddaE neurons expressing Hsap\ATXN3tr.Q78.UAS.Tag:HA under the control of Scer\GAL4221 do not show significant changes in growing microtubules within dendrites, as compared to neurons in controls. Expression under the control of Scer\GAL4GMR.long does not induce any visible eye defect compared to controls.

      (Chen et al., 2012)

      Larvae expressing Hsap\ATXN3tr.Q27.Scer\UAS.T:Ivir\HA1 in class IV da neurons under the control of Scer\GAL4ppk.PG do not exhibit any detectable dendritic abnormalities. Dendritic branching and complexity is similar to controls.

      (Lee et al., 2011)

      Expression of Hsap\MJDtr.Q27.Scer\UAS.T:Ivir\HA1 in the developing eye under the control of Scer\GAL4GMR.PF does not result in any significant structural defects in the eye.

      (Park et al., 2011)

      Scer\GAL4elav-C155-driven expression of Hsap\MJDtr.Q27.Scer\UAS.T:Ivir\HA1 does not have any adverse effect on survival or longevity of flies.

      (Mallik and Lakhotia, 2009)

      Expression of Hsap\ATXN3tr.Q27.Scer\UAS.T:Ivir\HA1 in the nervous system under the control of Scer\GAL4elav.PLu has no effect on the survival rate of flies during their development to adults.

      (Fujikake et al., 2008)

      Expression of Hsap\ATXN3tr.Q27.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PU does not cause degeneration or pigment loss in the adult eye.

      (Huen and Chan, 2005)

      Expression of Hsap\ATXN3tr.Q27.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4elav.PU, Scer\GAL4repo-M1B or Scer\GAL4Appl.G1a leads to a reduction in adult lifespan.

      Expression of Hsap\ATXN3tr.Q27.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4repo-M1B, but not Scer\GAL4Appl.G1a, results in progressive neurodegeneration, with formation of vacuoles in the brain.

      Flies expressing Hsap\ATXN3tr.Q27.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Appl.G1a or Scer\GAL4repo-M1B exhibit a progressive deficit in phototaxis, but no defects in walking speed or activity, as compared to controls.

      (Kretzschmar et al., 2005)

      Co-expression of Hsap\MJDtr.Q27.Scer\UAS.T:Ivir\HA1 does not suppress the eye degeneration phenotype caused by Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF.

      (Warrick et al., 2005)

      When driven in neurons no increase in apoptosis is seen.

      (Gunawardena et al., 2003)

      Expression of Hsap\MJDQ27.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF has no effect on eye morphology.

      (Bonini, 1999)

      Expression of Hsap\MJDQ27.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF, Scer\GAL4elav-C155, Scer\GAL4how-24B or Scer\GAL4dpp.blk1 has no phenotypic effect.

      (Warrick et al., 1998)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Adults co-expressing Hsap\ATXN3tr.Q27.UAS.Tag:HA and slmbNIG.3412R under the control of Scer\GAL4GMR.PU display progressive eye defects, including loss of pigmentation. These eye defects are not observed when Hsap\ATXN3tr.Q27.UAS.Tag:HA is co-expressed in combination with either Cul1GD9650, Cul1NIG.1877R, Cul2GD8913, Cul3GD7408, Cul4GD14006, Cul5GD8193, FipoQNIG.2010R, FipoQKK108266, agoNIG.15010R, Kdm2GD7173, CG11658NIG.11658R, Fbxl4GD5204, CG32085GD10452, pallNIG.3428R, Fbxl7GD11255, CG4911NIG.4911R, CG5961NIG.5961R, CG6758GD12027, jetGD14053, CG9003GD13533, Fbw5NIG.9144R, FBXO11GD14222, Skp2GD5142, PpaGD14324, or Rca1NIG.10800R.

      (Chen et al., 2019)

      Expression of Hsap\MJDtr.Q27.Scer\UAS.T:Ivir\HA1 and Mmus\PrnpP101L.Scer\UAS.T:Hsap\PRNP-3F4 in the developing eye under the control of Scer\GAL4GMR.PF does not result in any significant structural defects in the eye.

      (Park et al., 2011)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      Hsap\ATXN3tr.Q27.Scer\UAS.T:Ivir\HA1
      Hsap\ATXN3tr.Q27.UAS.Tag:HA
      Hsap\MJDQ27.Scer\UAS.T:Ivir\HA1
      Hsap\MJDQ27.UAS.T:HA1
      Hsap\MJDtr.Q27.Scer\UAS.T:Ivir\HA1
      Name Synonyms
      Secondary FlyBase IDs
        References (30)