FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\IKKε5
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General Information
Symbol
Dmel\IKKε5
Species
D. melanogaster
Name
FlyBase ID
FBal0091354
Feature type
allele
Associated gene
Dmel\IKKε
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Oshima et al., 2006)
Mutagen
ethyl methanesulfonate
(Kozlova et al., 1998)
Progenitor genotype
Cytology
Description

Nucleotide substitution: G?A.

(Kuranaga et al., 2006)

This mutation is within the putative kinase domain of the expressed protein.

(Kuranaga et al., 2006)

Amino acid replacement: D160N.

(Kuranaga et al., 2006, Oshima et al., 2006, Shapiro and Anderson, 2006)

The amino acid replacement, caused by a point mutation, is found in the kinase domain of ik2.

(Shapiro and Anderson, 2006)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:20,676,854..20,676,854 [+]
Nucleotide change:

G20676854A

Reported nucleotide change:

G?A

Amino acid change:

D160N | IKKepsilon-PB; D160N | IKKepsilon-PC

Reported amino acid change:

D160N

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Kuranaga et al., 2006, Oshima et al., 2006, Shapiro and Anderson, 2006)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Apoptosis occurs normally in l(2)38Ea36/l(2)38Ea66 and l(2)38Ea46/l(2)38Ea66 embryos.

      (Kuranaga et al., 2006)

      The tracheal branching of l(2)38Ea66 embryos occurs normally with apparently normal cell number. At stage 17, the terminal branches show a variety of abnormal morphologies. One class of abnormalities is the duplication of terminal cells. The other classes show persistent filopodia formation in the dorsal side of the terminal cells and the ectopic accumulation of F actin dots, as well as the misorientation and bifurcation of the actin core and the terminal branch.

      (Oshima et al., 2006)

      At low temperature and in uncrowded culture conditions, rare escaper ik25 adults (<1%) are observed, but they die shortly after eclosion. These escapers often exhibit abnormal bristles, both the interommatidial and the humeral bristles are affected. At high magnification, the actin footprints along the length of the bristle shaft in ik25 escaper adult eyes appears less organised than those in wild-type bristles. In wild-type, interommatidial bristles are found at alternating vertices, whereas in ik25 mutants these bristles are often duplicated at a single vertex and are misshapen and kinked.

      (Shapiro and Anderson, 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      IKKε5 is a suppressor of eye phenotype of rprGMR.PH

      (Kuranaga et al., 2006)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      l(2)38Ea66 suppresses the eye ablation phenotype of flies that express rprGMR.PH.

      (Kuranaga et al., 2006)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      The allele incorrectly referred to as 'ikkε[66]' in FBrf0228877 actually corresponds to IKKε1 (also known as 'ikkε[36]'). The confusion arose due to misannotation of a laboratory stock list.

      (Otani et al., 2015)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (10)
      Reported As
      Symbol Synonym
      66
      (Oshima et al., 2006)
      APTX766
      (Kuranaga et al., 2006)
      DmIKKεD160N
      (Kuranaga et al., 2006)
      Ea66
      (Shapiro and Anderson, 2006)
      IKKepsilon5
      (Kahsai and Cook, 2018)
      IKKε5
      ik25
      (Shapiro and Anderson, 2006)
      ik266
      (Matthews, 2007.10.18)
      ikkε66
      (Otani et al., 2015)
      l(2)38Ea66
      (Kuranaga et al., 2006, Oshima, 2006)
      Name Synonyms
      Secondary FlyBase IDs
        References (10)