FlyBase Allele Report: Dmel\Ras85D[V12.hs]

General Information

Symbol

Dmel\Ras85D^V12.hs

Species

D. melanogaster

Name

FlyBase ID

FBal0091660

Feature type

allele

Associated gene

Dmel\Ras85D

Associated Insertion(s)

Carried in Construct

P{hsRas1.V12}

Also Known As

hs-Ras1^V12

Key Links

Transgenic product class

missense_variant

(Miller and Cagan, 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Miller and Cagan, 1998)

Mutagen

in vitro construct

(Miller and Cagan, 1998)

Progenitor genotype

Carried in construct

P{hsRas1.V12}

Cytology

Description

Construct: Ras85D^V12 coding region expression is driven by a heat shock promoter.

(Miller and Cagan, 1998)

Allele components

Component

Use(s)

Encoded product / tool

Ras85D

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

This somatic variant has been found associated with cancer in each of the three paralogous genes in human (KRAS, HRAS, NRAS).

Phenotypic Data

Phenotypic Class

visible | heat sensitive

(Martin-Blanco et al., 1999)

Phenotype Manifest In

Detailed Description

Statement

Reference

Ras85D^V12.hs heterozygous flies maintained at 25[o]C exhibit normal life spans and locomotor activity.

Overexpression of Ras85D^V12.hs does not affect superoxide production.

(Tong et al., 2007)

Expression of Ras85D^V12.hs using heat shock at 24 hours after puparium formation results in extra lattice cells in the retina; there are an average of 18.5 +/- 0.3 cells per ommatidial unit (compared to 12 cells per ommatidial unit in wild-type animals).

(Rusconi et al., 2004)

Heat shock expression of Ras85D^V12.hs can lead to a morphogenetic furrow arrest phenotype in the developing eye.

(Hayashi and Saigo, 2001)

Expression of Ras85D^V12.hs during late larval development using heat shock results in flies that show a loss of wing veins.

(Martin-Blanco et al., 1999)

Ablation of cone cells in four adjacent developing ommatidia at 20 hrs APF causes death of interommatidial precursor cells in wild type. This IPC death is blocked by N^fa-g62 or Ras85D^V12.hs.

(Miller and Cagan, 1998)

Transient expression by heat shock during early pupal development (0-5.5 hr APF) causes an increase in cone-like cells. Heat shock during later pupal development (20-40 hr APF) has no effect on cone cells but causes an increase in the number of secondary and tertiary pigment cells.

(Sawamoto et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Ras85D^V12.hs has ommatidium phenotype, enhanceable by klu[+]/klu^unspecified

(Rusconi et al., 2004)

Suppressor of

Statement

Reference

Ras85D^V12.hs is a suppressor of cone cell phenotype of aos^GMR.PS

(Sawamoto et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

klu^unspecified/+ enhances the extra cell phenotype seen in the retina of animals expressing Ras85D^V12.hs using heat shock at 24 hours after puparium formation; the double mutant animals have an average of 20.03 +/- 0.2 cells per ommatidial unit (compared to an average of 18.5 +/- 0.3 cells per ommatidial unit in animals expressing Ras85D^V12.hs at the same time in a klu⁺ background).

(Rusconi et al., 2004)

Flies carrying both Ras85D^V12.hs and argos^GMR.PS show a normal number of cone cells in most ommatidia.

(Sawamoto et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

N. Perrimon

(Miller and Cagan, 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Dras1^Val12

(Spencer et al., 1998)

Ras85D^V12.hs

ras^v12

(Gorski et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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