FlyBase Allele Report: Dmel\ap[UAS.cOa]

General Information

Symbol

Dmel\ap^UAS.cOa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of O'Keefe

FlyBase ID

FBal0093165

Feature type

allele

Associated gene

Dmel\ap

Associated Insertion(s)

Carried in Construct

P{UAS-ap.O}

Also Known As

UAS-ap

Key Links

Transgenic product class

wild_type

(O'Keefe et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(O'Keefe et al., 1998)

Mutagen

in vitro construct

(O'Keefe et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-ap.O}

Cytology

Description

UAS regulatory sequences drive ap expression.

(O'Keefe et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of ap^Scer\UAS.cOa using Scer\GAL4^how-24B leads to malformation of the abdominal lateral transverse muscles and loss of the segment border muscle.

(Müller et al., 2010)

Expression driven by Scer\GAL4¹¹⁵¹ in the IFMs disrupts their differentiation.

(Ghazi et al., 2000)

Flies expressing ap^Scer\UAS.cOa under the control of Scer\GAL4^ap-md544 show no wing defects.

(van Meyel et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

ap^UAS.cOa/Scer\GAL4^αTub84B.PZ is a suppressor | somatic clone of decreased occurrence of cell division | larval stage phenotype of vn^L6/vn^ddd-4

(Zecca and Struhl, 2002)

ap^UAS.cOa, Scer\GAL4^ap-md544 is a suppressor of visible phenotype of Chi^{ΔDD.UAS.Tag:MYC}, Scer\GAL4^ap-md544

(van Meyel et al., 1999)

ap^UAS.cOa, Scer\GAL4^ap-md544 is a suppressor of visible phenotype of Chi^{FL.UAS.Tag:MYC}, Scer\GAL4^ap-md544

(van Meyel et al., 1999)

ap^UAS.cOa/Scer\GAL4^ap-md544 is a suppressor of visible phenotype of Scer\GAL4^ap-md544, tup^ΔHD.UAS

(O'Keefe et al., 1998)

NOT Suppressor of

Statement

Reference

ap^UAS.cOa, Scer\GAL4^ap-md544 is a non-suppressor of visible phenotype of Chi^{ΔLID.UAS.Tag:MYC}, Scer\GAL4^ap-md544

(van Meyel et al., 1999)

Phenotype Manifest In

Suppressor of

Statement

Reference

ap^UAS.cOa/Scer\GAL4^ap-md544 is a suppressor of wing phenotype of Scer\GAL4^ap-md544, tup^UAS.cTa

(Biryukova and Heitzler, 2005)

ap^UAS.cOa, Scer\GAL4^ap-md544 is a suppressor of tarsal segment 4 phenotype of Bar^UAS.cKa, Scer\GAL4^ap-md544

(Pueyo and Couso, 2004)

ap^UAS.cOa/Scer\GAL4^αTub84B.PZ is a suppressor of wing disc phenotype of vn^L6/vn^ddd-4

(Zecca and Struhl, 2002)

ap^UAS.cOa, Scer\GAL4^ap-md544 is a suppressor of wing phenotype of Chi^{FL.UAS.Tag:MYC}, Scer\GAL4^ap-md544

(van Meyel et al., 1999)

ap^UAS.cOa, Scer\GAL4^ap-md544 is a suppressor of wing phenotype of Chi^{ΔDD.UAS.Tag:MYC}, Scer\GAL4^ap-md544

(van Meyel et al., 1999)

ap^UAS.cOa/Scer\GAL4^ap-md544 is a suppressor of wing phenotype of Scer\GAL4^ap-md544, tup^ΔHD.UAS

(O'Keefe et al., 1998)

NOT Suppressor of

Statement

Reference

ap^UAS.cOa/Scer\GAL4^ap-md544 is a non-suppressor of tarsal segment 4 phenotype of Scer\GAL4^ap-md544, tup^UAS.cTa

(Pueyo and Couso, 2004)

ap^UAS.cOa, Scer\GAL4^ap-md544 is a non-suppressor of wing phenotype of Chi^{ΔLID.UAS.Tag:MYC}, Scer\GAL4^ap-md544

(van Meyel et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Coexpression of the ap^Scer\UAS.cOa transgene rescues the rudimentary wing phenotype of Scer\GAL4^ap-md544>tup^Scer\UAS.cTa flies.

(Biryukova and Heitzler, 2005)

The loss of tarsal segment 4 in the legs of tup^Scer\UAS.cTa; Scer\GAL4^ap-md544 flies is not suppressed by ap^Scer\UAS.cOa. The loss of tarsal segment 4 in the legs of B^Scer\UAS.cKa; Scer\GAL4^ap-md544 flies is suppressed by ap^Scer\UAS.cOa.

(Pueyo and Couso, 2004)

Clones expressing ap^Scer\UAS.cOa under the control of Scer\GAL4^αTub84B.PZ induced in the first larval instar can rescue growth and differentiation vn^L6/vn^ddd-4 wing discs.

(Zecca and Struhl, 2002)

Suppresses the wing defects caused by expression of Chi^{FL.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ap-md544. Partially suppresses the wing defects caused by expression of Chi^{ΔDD.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ap-md544. Does not suppress the wing defects caused by expression of Chi^{ΔLID.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ap-md544.

(van Meyel et al., 1999)

Suppresses the wing phenotype of flies expressing tup^{ΔHD.Scer\UAS} under the control of Scer\GAL4^ap-md544.

(O'Keefe et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

ap^UAS.cOa/Scer\GAL4^ap-md544 rescues ap^md544/Df(2R)nap1

(Pueyo and Couso, 2004)

Partially rescues

ap^UAS.cOa/Scer\GAL4¹¹⁵¹ partially rescues ap⁴

(Ghazi et al., 2000)

ap^UAS.cOa/Scer\GAL4^pnr-MD237 partially rescues ap⁴

(Ghazi et al., 2000)

ap^UAS.cOa/Scer\GAL4^ap-md544 partially rescues ap^md544/ap^P44

(O'Keefe et al., 1998)

Comments

Reduction and fusion of the fourth tarsal segment in ap^md544/Df(2R)nap1 animals is rescued by ap^Scer\UAS.cOa.

(Pueyo and Couso, 2004)

When expression is driven by Scer\GAL4¹¹⁵¹, partially rescues the the DFM loss phenotype of ap⁴ mutants (though a large single muscle mass develops as opposed to four discrete fibres) but does not rescue the IFM phenotype. When expression is driven by Scer\GAL4^pnr-MD237, the DLM defects of ap⁴ mutants are significantly rescued while DFM fibres remain disorganised. Muscle volume is significantly restored.

(Ghazi et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

42222

w^*; P{UAS-ap.O}8a

42223

w^*; P{UAS-ap.O}8b

Notes on Origin

Discoverer