FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\vgUAS.cZa
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General Information
Symbol
Dmel\vgUAS.cZa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Zider
FlyBase ID
FBal0093754
Feature type
allele
Associated gene
Dmel\vg
Associated Insertion(s)
Carried in Construct
P{UAS-vg.Z}
Also Known As
UAS-vg, UAS-vgZ, UASvg
Key Links
Transgenic product class
cDNA
(Paumard-Rigal et al., 1998)
wild_type
(Paumard-Rigal et al., 1998)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Paumard-Rigal et al., 1998)
wild_type
(Paumard-Rigal et al., 1998)
Mutagen
in vitro construct
(Paumard-Rigal et al., 1998)
Progenitor genotype
Carried in construct
P{UAS-vg.Z}
Cytology
Description

UASt regulatory sequences drive expression of a vg cDNA lacking the first 800bp of 5' untranslated sequence.

(Paumard-Rigal et al., 1998)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
vg
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      antenna, with Scer\GAL4Dll-md23

      (Baena-Lopez and Garcia-Bellido, 2003)

      genitalia, with Scer\GAL4Dll-md23

      (Baena-Lopez and Garcia-Bellido, 2003)

      leg | somatic clone, with Scer\GAL4Ubx.PdC

      (Baena-Lopez and Garcia-Bellido, 2003)

      mesonotum | somatic clone, with Scer\GAL4Ubx.PdC

      (Baena-Lopez and Garcia-Bellido, 2003)

      mesothoracic leg, with Scer\GAL4Dll-md23

      (Baena-Lopez and Garcia-Bellido, 2003)

      metathoracic leg, with Scer\GAL4Dll-md23

      (Baena-Lopez and Garcia-Bellido, 2003)

      proboscis, with Scer\GAL4Dll-md23

      (Baena-Lopez and Garcia-Bellido, 2003)

      prothoracic leg, with Scer\GAL4Dll-md23

      (Baena-Lopez and Garcia-Bellido, 2003)

      wing, with Scer\GAL4ptc-559.1

      (Paumard-Rigal et al., 1998)

      wing | ectopic, with Scer\GAL4Dll-md23

      (Baena-Lopez and Garcia-Bellido, 2003)

      wing | somatic clone, with Scer\GAL4Ubx.PdC

      (Baena-Lopez and Garcia-Bellido, 2003)

      wing disc, with Scer\GAL4ap-md544

      (Baena-Lopez and Garcia-Bellido, 2006)

      wing disc, with Scer\GAL4en-e16E

      (Delanoue et al., 2004)

      wing disc, with Scer\GAL4nub.PK

      (Baena-Lopez and Garcia-Bellido, 2006)

      wing disc, with Scer\GAL4ptc-559.1

      (Delanoue et al., 2002)

      wing disc, with Scer\GAL4salm.EPv

      (Baena-Lopez and Garcia-Bellido, 2006)

      wing hair | ectopic | somatic clone, with Scer\GAL4Ubx.PdC

      (Baena-Lopez and Garcia-Bellido, 2003)

      wing margin bristle | ectopic, with Scer\GAL4Dll-md23

      (Baena-Lopez and Garcia-Bellido, 2003)

      wing sensillum & sensillum campaniformium | ectopic, with Scer\GAL4Dll-md23

      (Baena-Lopez and Garcia-Bellido, 2003)

      wing vein | ectopic, with Scer\GAL4Dll-md23

      (Baena-Lopez and Garcia-Bellido, 2003)
      Detailed Description
      Statement
      Reference

      In a background in which vgScer\UAS.cZa is expressed under the control of Scer\GAL4salm-EPv, Scer\GAL4ap-md544 or Scer\GAL4nub.PK, cell clones fail to grow preferentially along the proximal-distal axis (as they do in a wild type background) and the orientation of cell divisions is perturbed.

      (Baena-Lopez and Garcia-Bellido, 2006)

      Expression of vgScer\UAS.cZa in the posterior compartment of the wing imaginal disc under the control of Scer\GAL4en-e16E results in a 15% reduction in the number of cells in G1 compared to the anterior compartment of the disc, and increased cell proliferation. There is no difference in average cell size compared to control cells.

      (Delanoue et al., 2004)

      Expression of vgScer\UAS.cZa under the control of Scer\GAL4Dll-md23 results in the autonomous transformation of distal territories of the appendages (proboscis, first and second pairs of legs and genitalia) into tissues with characteristics of the wing blade. In the first and second pairs of legs, these transformations produce trichomes, anteroposterior and dorsoventral wing margin chaetae, wing veins and campaniform sensilla. The transformed legs have large ventral wing territories and small dorsal territories encircled by wing margin. In the third pair of legs, transformation to haltere tissues is seen. Expression of vgScer\UAS.cZa under the control of Scer\GAL4Dll-md23 results in the transformation of antenna with anterior and posterior wing margin elements. Expression of vgScer\UAS.cZa under the control of Scer\GAL4vg.PM does not result in cuticular transformation of the eye. Expression of vgScer\UAS.cZa under the control of Scer\GAL4Ubx.PdC in clones in the wing results in tubular, perpendicular outgrowths to the wing surface. The outgrowths contain both clone cells and surrounding wild-type cells. The clones are frequently located at the tip of the outgrowth but they can also grow along the lateral zones. In those clones that appear in central parts of the wing, all the cells of the outgrowth and the clone always show a differentiation corresponding to wing blade trichomes. Clones near the wing margin may differentiate marginal chaetae. Clones in the notum expressing vgScer\UAS.cZa under the control of Scer\GAL4Ubx.PdC contain wing blade trichomes and notum chaetae in a "salt and pepper" distribution. Clones in the leg expressing vgScer\UAS.cZa under the control of Scer\GAL4Ubx.PdC have "mixed" cuticular differentiation patterns, with leg chaetae being mixed in a "salt and pepper" distribution with wing blade trichomes.

      (Baena-Lopez and Garcia-Bellido, 2003)

      Expression of vgScer\UAS.cZa under the control of Scer\GAL4ptc-559.1 causes deformation of the wing disc.

      (Delanoue et al., 2002)

      Animals expressing vgScer\UAS.cZa under the control of Scer\GAL4ptc-559.1 usually die at the early pupal stage. Animals that die at the late pupal stage show extensive wing-like outgrowths associated with a disorganisation and reduction of the wings. The formation of ectopic wing-like outgrowths caused by expression of vgScer\UAS.cZa under the control of Scer\GAL4ptc-559.1 is more strongly reduced in a vg1, vg83b27 or vgnull homozygous background than in the corresponding heterozygous vg1, vg83b27 or vgnull background.

      (Paumard-Rigal et al., 1998)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Suppressor of
      Statement
      Reference

      vgUAS.cZa/Scer\GAL4vg.int2.1 is a suppressor of wing phenotype of Scer\GAL4vg.int2.1, dapUAS.cLa, vg[+]/vgnull

      (Delanoue et al., 2004)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of vgScer\UAS.cZa in flies expressing dapScer\UAS.cLa at the dorsal/ventral wing boundary under the control of Scer\GAL4vg.int2.1 in a vgnull/+ mutant background rescues wing morphology, and mitotic activity of cells is restored in this region.

      (Delanoue et al., 2004)

      The transformation of the legs and antenna seen in flies expressing vgScer\UAS.cZa under the control of Scer\GAL4Dll-md23 is strongly reduced by coexpression of wg-C.Scer\UAS. Co-expression of vgScer\UAS.cZa and wgScer\UAS.cLa under the control of Scer\GAL4vg.PM causes the transformation of the eye into wing blade, resulting in outgrowths with histotypical characteristics typical of wing blade. Co-expression of vgScer\UAS.cZa and wgScer\UAS.cLa under the control of Scer\GAL4Ubx.PdC in clones in the wing imaginal disc leads to the formation of tubular and perpendicular outgrowths from the wing blade. The size of the outgrowths is larger than in clones expressing vgScer\UAS.cZa under the control of Scer\GAL4Ubx.PdC and is dependent on the distance from the wing margin. All the cells of the wg-vg expressing clones are differentiated into wing margin sensory elements, whereas non-autonomous territories of the outgrowth are differentiated into wing blade trichomes. Co-expression of vgScer\UAS.cZa and wgScer\UAS.cLa under the control of Scer\GAL4Ubx.PdC in clones in the wing hinge or notum cause transformation phenotypes; the cells autonomously differentiate into wing margin and non-autonomously differentiate into wing blade trichomes. Co-expression of vgScer\UAS.cZa and wgScer\UAS.cLa under the control of Scer\GAL4Ubx.PdC in clones in the leg results in a "mixed" phenotype with leg chaetae mixed with wing blade trichomes in a "salt and pepper" distribution. Co-expression of vgScer\UAS.cZa and wgScer\UAS.cLa under the control of Scer\GAL4Ubx.PdC in clones in the eye results in the cells autonomously differentiating into sensory elements of the wing margin.

      (Baena-Lopez and Garcia-Bellido, 2003)

      Marker expression data suggests that the presence of vgScer\UAS.cZa transforms wgScer\UAS.cGa; Scer\GAL4αTub84B.PL somatic clone cells in the peripodial epithelium from wing hinge like to wing pouch like.

      (Baena-Lopez et al., 2003)

      Females expressing vgScer\UAS.cZa under the control of Scer\GAL4ptc-559.1 in a sdETX4/+ background die in the late pupal stage. They show ectopic wing-like outgrowths at the posterior of the head capsule, with strong deformation of the eye. Males expressing vgScer\UAS.cZa under the control of Scer\GAL4ptc-559.1 in a hemizygous sdETX4 background do not show such extensive wing-like outgrowths and show only slight deformation of the eye. These males can emerge from the pupae, although they die within a few hours.

      (Paumard-Rigal et al., 1998)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      vgUAS.cZa rescues vgnull

      (Delanoue et al., 2004)

      vgUAS.cZa/Scer\GAL4vg.PU rescues vg83b27

      (van de Bor et al., 1999)
      Partially rescues

      vgUAS.cZa/Scer\GAL4ap-md544 partially rescues vgnull

      (Bernard et al., 2003)

      vgUAS.cZa/Scer\GAL4vg.PU partially rescues vg1

      (van de Bor et al., 1999)
      Comments

      Expression of vgScer\UAS.cZa under the control of Scer\GAL4ptc-559.1 in vgnull mutant discs increases the number of cells in S-phase and results in overgrowth of the disc along the anterior/posterior axis. An increase in cells in M-phase is observed.

      (Delanoue et al., 2004)

      Scer\GAL4ap-md544; vgScer\UAS.cZa; partially but significantly rescues the adult muscle phenotype vgnull/vgnull flies.

      (Bernard et al., 2003)

      The wing defects seen in vg83b27 flies are completely rescued by expression of vgScer\UAS.cZa under the control of Scer\GAL4vg.PU.

      The wing defects seen in vg1 flies are partially rescued by expression of vgScer\UAS.cZa under the control of Scer\GAL4vg.PU; nicks are seen at the wing tips in the rescued flies.

      (van de Bor et al., 1999)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      vgScer\UAS.cZa
      vgUAS.cZa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Zider
      (Paumard-Rigal et al., 1998)
      Secondary FlyBase IDs
        References (9)