FlyBase Allele Report: Dmel\cry[UAS.cEa]

General Information

Symbol

Dmel\cry^UAS.cEa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Emery

FlyBase ID

FBal0094553

Feature type

allele

Associated gene

Dmel\cry

Associated Insertion(s)

Carried in Construct

P{UAS-cry.E}

Also Known As

UAS-cry

Key Links

Transgenic product class

cDNA

(Emery et al., 1998)

wild_type

(Emery et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Emery et al., 1998)

wild_type

(Emery et al., 1998)

Mutagen

in vitro construct

(Emery et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-cry.E}

Cytology

Description

Expression of a cry cDNA is governed by UASt regulatory sequences.

(Emery et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

cry

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA with Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}

(Jarabo et al., 2022)

CEA with btl::Egfr^λ.UAS

(Jarabo et al., 2022)

CEA with Myc^UAS.cUa

(Jarabo et al., 2022)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm, with Scer\GAL4^tim.PE

(Emery et al., 2000, Emery et al., 1998)

abnormal locomotor rhythm

(Yoshii et al., 2009)

abnormal locomotor rhythm, with Scer\GAL4^P0.5.Pdf

(Collins et al., 2006)

Phenotype Manifest In

Detailed Description

Statement

Reference

Flies expressing Scer\GAL4^tim.Scer\UAS driven cry^Scer\UAS.cEa show significantly longer free-running periods than wild-type flies under blue light.

Responsiveness of flies expressing Scer\GAL4^tim.Scer\UAS driven cry^Scer\UAS.cEa to magnetic fields is altered compared to wild-type flies. This response is blue light dependent. After experimental exposure to a 300 microT magnetic field, most of the Scer\GAL4^tim.Scer\UAS driven cry^Scer\UAS.cEa expressing flies become arrhythmic and the remaining ones show large changes in their circadian free-running periods.

(Yoshii et al., 2009)

Flies expressing cry^Scer\UAS.cEa, under the control of Scer\GAL4^P0.5.Pdf, exhibit low levels of arrhythmicity (approximately 16.7% versus 13%).

(Collins et al., 2006)

When cry^Scer\UAS.cEa is driven in the lateral neurons by Scer\GAL4^tim.PE, the mutant flies become hypersensitive to low-intensity illumination, especially at Zeitgeber time (ZT) 15, though a modest but reproducible effect is seen at ZT21.

(Emery et al., 2000)

When expression is driven by Scer\GAL4^tim.PE, hypersensitive circadian responses to light are produced. The flies are much more sensitive to light at low intensities. The effect is particularly strong at ZT15, suggesting that cry levels are normally limiting at low light intensities. The magnitude of the effect is more variable at ZT21, in the advance zone.

(Emery et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^P0.5.Pdf, cry^UAS.cEa has abnormal locomotor rhythm phenotype, non-enhanceable by Scer\GAL4^P0.5.Pdf/per^UAS.cYa

(Collins et al., 2006)

NOT Enhancer of

Statement

Reference

Scer\GAL4^P0.5.Pdf/cry^UAS.cEa is a non-enhancer of abnormal locomotor rhythm phenotype of Scer\GAL4^P0.5.Pdf, per^UAS.cYa

(Collins et al., 2006)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Flies expressing per^Scer\UAS.cYa and cry^Scer\UAS.cEa, under the control of Scer\GAL4^P0.5.Pdf, exhibit low levels of arrhythmicity (approximately 15.7% versus 13%).

(Collins et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

cry^UAS.cEa/Scer\GAL4^tim.PE rescues cry^b

(Picot et al., 2007)

cry^UAS.cEa/Scer\GAL4^Mai179 rescues cry^b

(Picot et al., 2007)

cry^UAS.cEa, Scer\GAL4^Mai179, Scer\GAL80^Pdf.PS rescues cry^b

(Picot et al., 2007)

cry^UAS.cEa, Scer\GAL4^tim.PE, Scer\GAL80^Pdf.PS rescues cry^b

(Picot et al., 2007)

cry^UAS.cEa/Scer\GAL4^tim.PE rescues cry^b

(Emery et al., 2000)

cry^UAS.cEa/Scer\GAL4^tim.PE rescues cry^b

(Emery et al., 2000)

Partially rescues

cry^UAS.cEa/Scer\GAL4^P2.4.Pdf partially rescues cry^b

(Emery et al., 2000)

Fails to rescue

cry^UAS.cEa/Scer\GAL4^P2.4.Pdf fails to rescue cry^b

(Picot et al., 2007)

cry^UAS.cEa/Scer\GAL4^ninaE.PD fails to rescue cry^b

(Emery et al., 2000)

Comments

In contrast to wild-type animals, cry^b mutants expressing Scer\GAL4^P2.4.Pdf driven cry^Scer\UAS.cEa do display circadian locomotor activity rhythms under constant light conditions.

Similarly to wild-type flies, the majority of cry^b mutants expressing Scer\GAL4^Mai179 driven cry^Scer\UAS.cEa do not display circadian locomotor activity rhythms under constant light conditions regardless of the presence or absence of Scer\GAL80^Pdf.PS in the genotype.

Similarly to wild-type animals, most of the cry^b mutants expressing Scer\GAL4^tim.PE driven cry^Scer\UAS.cEa do not display locomotor activity rhythms under constant light conditions regardless of the presence or absence of Scer\GAL80^Pdf.PS in the genotype.

(Picot et al., 2007)

The behavioural rhythmicity seen in cry^b flies under intense constant light (wild-type flies are arrhythmic under such conditions) is rescued by expression of cry^Scer\UAS.cEa under the control of Scer\GAL4^tim.PE; the rescued flies are arrhythmic.

(Emery et al., 2000)

Expression of cry^Scer\UAS.cEa under the control of Scer\GAL4^tim.PE rescues the circadian phase-resetting defect seen in cry^b flies and restores the wild-type arrhythmicity behaviour that is defective in cry^b flies. When cry^Scer\UAS.cEa is driven by Scer\GAL4^P2.4.Pdf, the phase setting defect is rescued at Zeitgeber time (ZT)21, but only partially at ZT15, and the wild-type arrhythmicity behaviour is only partially restored. When cry^Scer\UAS.cEa is driven by Scer\GAL4^ninaE.PD, rescue of neither phenotype is seen. per^s; cry^b double mutant flies manifest pseudo free-running behaviour under LD conditions, indicative of an entrainment deficit. On addition of cry^Scer\UAS.cEa driven by Scer\GAL4^tim.PE, per^s; cry^b flies show an activity pattern that resembles very closely that of per^s flies. On addition of cry^Scer\UAS.cEa driven by Scer\GAL4^tim.PE, norpA³⁹; cry^b flies show an activity pattern that resembles very closely that of norpA³⁹ flies.