FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\brkUAS.cJa
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General Information
Symbol
Dmel\brkUAS.cJa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Jazwinska
FlyBase ID
FBal0095494
Feature type
allele
Associated gene
Dmel\brk
Associated Insertion(s)
Carried in Construct
P{UAS-brk.J}
Also Known As
UAS-brk
Key Links
Transgenic product class
cDNA
(Jazwinska et al., 1999)
wild_type
(Jazwinska et al., 1999)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Jazwinska et al., 1999)
wild_type
(Jazwinska et al., 1999)
Mutagen
in vitro construct
(Jazwinska et al., 1999)
Progenitor genotype
Carried in construct
P{UAS-brk.J}
Cytology
Description

Expression of brk cDNA is governed by UASt regulatory sequences.

(Jazwinska et al., 1999)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
brk
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      dorsal vessel wall cell, with Scer\GAL4how-24B, Scer\GAL4twi.PG

      (Klinedinst and Bodmer, 2003)

      embryonic/first instar larval cuticle, with Scer\GAL4VP16.mat.αTub67C

      (Jazwinska et al., 1999)

      embryonic/larval crystal cell, with Scer\GAL4twi.PG

      (Milchanowski et al., 2004)

      embryonic abdominal dorsal sensory organ precursor cluster, with Scer\GAL4Kr.PM

      (Rusten et al., 2002)

      embryonic abdominal lateral sensory organ precursor cluster, with Scer\GAL4Kr.PM

      (Rusten et al., 2002)

      eye, with Scer\GAL4GMR.PF

      (Oh and Irvine, 2011)

      imaginal disc, with Scer\GAL4nub-AC-62

      (Martin et al., 2004)

      presumptive embryonic/larval peripheral nervous system, with Scer\GAL4Kr.PM

      (Rusten et al., 2002)

      wing, with Scer\GAL4bi-omb-Gal4

      (Jazwinska et al., 1999)

      wing, with Scer\GAL4Bx-MS1096

      (Cook et al., 2004)

      wing, with Scer\GAL4nub-AC-62

      (Martin et al., 2004)

      wing, with Scer\GAL4salm-459.2

      (Barrio et al., 2007)

      wing, with Scer\GAL4vg.PU

      (Yang et al., 2013)

      wing | heat sensitive, with Scer\GAL4C-765

      (Martin et al., 2004)

      wing disc, with Scer\GAL4Ubx.PdC

      (Moreno et al., 2002)

      wing disc | cell autonomous | third instar larval stage

      (Rogulja and Irvine, 2005)

      wing disc | cell non-autonomous | medial | third instar larval stage

      (Rogulja and Irvine, 2005)

      wing disc | somatic clone, with Scer\GAL4FRT.Act5C

      (Oh and Irvine, 2011)

      wing disc posterior compartment, with Scer\GAL4hh-Gal4

      (Martin et al., 2004)

      wing vein, with Scer\GAL4Bx-MS1096

      (Cook et al., 2004)

      wing vein, with Scer\GAL4C-765

      (Cook et al., 2004)

      wing vein, with Scer\GAL4salm-459.2

      (Barrio et al., 2007)

      wing vein, with Scer\GAL4vg.int2.1

      (Cook et al., 2004)
      Detailed Description
      Statement
      Reference

      Scer\GAL4vg.PU-driven expression of brkScer\UAS.cJa leads to severely underdeveloped, small and deformed wings with stout bristles.

      (Yang et al., 2013)

      Wing disc clones expressing brkScer\UAS.cJa under the control of Scer\GAL4Scer\FRT.Act5C grow poorly and are rarely recovered.

      Expression of brkScer\UAS.cJa under the control of Scer\GAL4GMR.PF produces flies with small eyes compared to controls.

      (Oh and Irvine, 2011)

      Expression of brkScer\UAS.cJa using Scer\GAL4salm-459.2 results in moderate wing size reduction and vein loss.

      (Barrio et al., 2007)

      Somatic clones of brkScer\UAS.cJa; Scer\GAL4Act5C.Switch.PR cells in mid-third instar wing discs show autonomous decreases in numbers of cells in S-phase after 14 hours treatment with 20μg/ml RU486. However, if the edges of these clones are located medially they cause a large increase in the of cells in S-phase adjacent to the medially located edge of the clone. No increase in cell death occurs prior to 26 hours after the initiation of RU486 treatment. A small increase in apoptosis is seen at 48 hours.

      (Rogulja and Irvine, 2005)

      brkScer\UAS.cJa; Scer\GAL4Bx-MS1096 flies have small wings in which some or all of the veins are shifted, truncated or completely missing. Wing veins L2 and L5 are particularly stringly affected. brkScer\UAS.cJa; Scer\GAL4C-765 flies have a range of venation phenotypes, including selective loss or displacement of L2 and L5 veins, a single central vein (L3) or complete loss of veins. In brkScer\UAS.cJa; Scer\GAL4vg.int2.1 flies the wing veins are crowded into the central region of the wing blade, close to the anterior-posterior compartment boundary.

      (Cook et al., 2004)

      brkScer\UAS.cJa; Scer\GAL4C-765 and brkScer\UAS.cJa; Scer\GAL4nub-AC-62 flies have reduced wings. The degree of size reduction in the wings of brkScer\UAS.cJa; Scer\GAL4C-765 flies varies with temperature - it is much more severe in animals raised at 25oC than 17oC. This is also apparent in discs - with severe size reduction being see in wing leg and haltere discs of third instar larvae raised at 29oC, and less in those raised at lower temperatures. There is a slight increase in the level of apoptosis in the wing pouch of late third instar brkScer\UAS.cJa; Scer\GAL4nub-AC-62 wing discs, but there is also a decrease in the rate of cell division in the pouch, as measured by BrdU incorporation or cell division marker expression. A similar decrease in proliferation, restricted to the posterior compartment, is seen in brkScer\UAS.cJa; Scer\GAL4hh-Gal4 wing discs.

      (Martin et al., 2004)

      Expression of brkScer\UAS.cJa under the control of Scer\GAL4twi.PG blocks crystal cell development. Expression of brkScer\UAS.cJa under the control of Scer\GAL4srp blocks crystal cell development.

      (Milchanowski et al., 2004)

      When brkScer\UAS.cJa is driven throughout the mesoderm by Scer\GAL4how-24B and Scer\GAL4twi.PG, there is a consierable reduction in cardiogenesis.

      (Klinedinst and Bodmer, 2003)

      Expression of brkScer\UAS.cJa under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 has no effect on the testes.

      (Shivdasani and Ingham, 2003)

      Clones in the wing disc expressing brkScer\UAS.cJa under the control of Scer\GAL4Ubx.PdC do not survive in the centre of the disc, surviving only in lateral regions.

      (Moreno et al., 2002)

      Expression of brkScer\UAS.cJa under the control of Scer\GAL4Kr.PM results in a reduced number of neurons in the dorsal and lateral peripheral nervous system. Segments expressing brkScer\UAS.cJa show no increase in apoptosis compared to other abdominal segments that are not expressing brkScer\UAS.cJa.

      (Rusten et al., 2002)

      When expression is governed by Scer\GAL4bi-omb-Gal4 the area of the wing blade is dramatically reduced, leaving the alula and costa largely unperturbed. Corresponding third larval instar discs are reduced and do not have the normal folded morphology.

      (Jazwinska et al., 1999)

      Embryos expressing brkScer\UAS.cJa under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 have a completely ventralised cuticle.

      (Jazwinska et al., 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Statement
      Reference

      Scer\GAL4vg.PU, brkUAS.cJa has visible | adult stage phenotype, suppressible | partially by panΔN.UAS/Scer\GAL4vg.PU

      (Yang et al., 2013)

      Scer\GAL4vg.PU, brkUAS.cJa has abnormal size | adult stage phenotype, suppressible | partially by panΔN.UAS/Scer\GAL4vg.PU

      (Yang et al., 2013)

      Scer\GAL4FRT.Act5C, brkUAS.cJa has decreased cell number | somatic clone phenotype, suppressible | partially by Scer\GAL4FRT.Act5C/ykiS168A.UAS.Tag:V5

      (Oh and Irvine, 2011)

      Scer\GAL4GMR.PF, brkUAS.cJa has abnormal size | adult stage phenotype, suppressible by tkvQ253D.UAS.cNc/MadUAS.Tag:FLAG, Scer\GAL4GMR.PF

      (Oh and Irvine, 2011)

      Scer\GAL4GMR.PF, brkUAS.cJa has visible | adult stage phenotype, suppressible by tkvQ253D.UAS.cNc/MadUAS.Tag:FLAG, Scer\GAL4GMR.PF

      (Oh and Irvine, 2011)

      Scer\GAL4GMR.PF, brkUAS.cJa has abnormal size | adult stage phenotype, suppressible by ykiS168A.UAS.Tag:V5/Scer\GAL4GMR.PF

      (Oh and Irvine, 2011)

      Scer\GAL4GMR.PF, brkUAS.cJa has visible | adult stage phenotype, suppressible by ykiS168A.UAS.Tag:V5/Scer\GAL4GMR.PF

      (Oh and Irvine, 2011)

      Scer\GAL4FRT.Act5C, brkUAS.cJa has decreased cell number | somatic clone phenotype, suppressible by mir-banGS/Scer\GAL4FRT.Act5C

      (Oh and Irvine, 2011)

      Scer\GAL4GMR.PF, brkUAS.cJa has abnormal size | adult stage phenotype, suppressible by mir-banGS/Scer\GAL4FRT.Act5C

      (Oh and Irvine, 2011)

      Scer\GAL4GMR.PF, brkUAS.cJa has visible | adult stage phenotype, suppressible by mir-banGS/Scer\GAL4FRT.Act5C

      (Oh and Irvine, 2011)

      Scer\GAL4FRT.Act5C, brkUAS.cJa has decreased cell number | somatic clone phenotype, suppressible by BacA\p35UAS.cHa, Scer\GAL4FRT.Act5C

      (Oh and Irvine, 2011)

      Scer\GAL4Ubx.PdC, brkUAS.cJa has cell lethal phenotype, suppressible by Scer\GAL4Ubx.PdC/pucUAS.cMa

      (Moreno et al., 2002)

      Scer\GAL4Ubx.PdC, brkUAS.cJa has cell lethal phenotype, suppressible by BacA\p35UAS.cHa, Scer\GAL4Ubx.PdC

      (Moreno et al., 2002)
      NOT suppressed by
      Other
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      NOT suppressed by
      Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The wing phenotype - severely underdeveloped, small and deformed wings with stout bristles - characteristic for flies expressing brkScer\UAS.cJa under the control of Scer\GAL4vg.PU is partially improved by co-expression of panΔN.Scer\UAS (which on its own causes wing notches).

      (Yang et al., 2013)

      Expression of ykiS168A.Scer\UAS.T:SV5\V5 partially suppresses the underproliferation seen when brkScer\UAS.cJa is expressed in wing disc clones under the control of Scer\GAL4Scer\FRT.Act5C. Co-expression of tkvQ253D.Scer\UAS.cNc suppresses the growth defects further, producing some clone overgrowth, whilst the addition of MadScer\UAS.T:Zzzz\FLAG increases clone overgrowth even further.

      Co-expression of tkvQ253D.Scer\UAS.cNc and MadScer\UAS.T:Zzzz\FLAG does not suppress the growth and survival defects seen when brkScer\UAS.cJa is expressed in wing disc clones under the control of Scer\GAL4Scer\FRT.Act5C.

      Co-expression of MadScer\UAS.T:Zzzz\FLAG and tkvQ253D.Scer\UAS.cNc suppresses the small eye phenotype seen when brkScer\UAS.cJa is expressed under the control of Scer\GAL4GMR.PF.

      Expression of ykiS168A.Scer\UAS.T:SV5\V5 suppresses the small eye phenotype seen when brkScer\UAS.cJa is expressed under the control of Scer\GAL4GMR.PF. When tkvQ253D.Scer\UAS.cNc is additionally expressed overgrown eyes are seen.

      Expression of banGS suppresses the underproliferation seen when brkScer\UAS.cJa is expressed in wing disc clones under the control of Scer\GAL4Scer\FRT.Act5C. The eye growth defects seen when brkScer\UAS.cJa is expressed under the control of Scer\GAL4GMR.PF are also rescued.

      (Oh and Irvine, 2011)

      The reduction in wing size seen in brkScer\UAS.cJa; Scer\GAL4nub-AC-62 flies is not suppressed by pucScer\UAS.cMa.

      (Martin et al., 2004)

      The addition of brkScer\UAS.cJa suppresses the ectopic eye phenotype seen in eyScer\UAS.cHa, Scer\GAL4bi-omb-Gal4 animals. The addition of brkScer\UAS.cJa suppresses the ectopic eye phenotype seen in eyScer\UAS.cHa, Scer\GAL4dpp.blk1 animals.

      (Kango-Singh et al., 2003)

      Co-expression of BacA\p35Scer\UAS.cHa allows the survival of clones expressing brkScer\UAS.cJa under the control of Scer\GAL4Ubx.PdC in the centre of the wing disc, although their proliferation is impaired. Co-expression of pucScer\UAS.cMa allows the survival of clones expressing brkScer\UAS.cJa under the control of Scer\GAL4Ubx.PdC in the centre of the wing disc.

      (Moreno et al., 2002)
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of BacA\p35Scer\UAS.cHa allows recovery of brkScer\UAS.cJa expressing clones under the control of Scer\GAL4Scer\FRT.Act5C in the larval wing disc.

      (Oh and Irvine, 2011)

      BacA\p35Scer\UAS.cHa does not suppress the reduction of wing pouch size seen in brkScer\UAS.cJa; Scer\GAL4nub-AC-62 wing discs - even when 2 copies of BacA\p35Scer\UAS.cHa are present.

      (Martin et al., 2004)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      brkScer\UAS.cJa
      brkUAS.cJa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Jazwinska
      (Jazwinska et al., 1999)
      Secondary FlyBase IDs
        References (34)