FlyBase Allele Report: Dmel\mge[B10]

General Information

Symbol

Dmel\mge^B10

Species

D. melanogaster

Name

FlyBase ID

FBal0096776

Feature type

allele

Associated gene

Dmel\mge

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Vaskova et al., 2000)

Progenitor genotype

Cytology

Description

Amino acid replacement: W94term.

(Vaskova et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:3,908,389..3,908,389 [-]

Nucleotide change:

G3908389A

Amino acid change:

W94term | mge-PA; W68term | mge-PB

Reported amino acid change:

W94term

Comment:

G to A nucleotide change at the second or third position of the wild type Trp codon leads to a nonsense mutation (exact site of mutation unspecified). The mutation was annotated at the second base of the codon.

(Vaskova et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased rate of movement | larval stage (with Df(3L)449)

(Vaskova et al., 2000)

decreased rate of movement | larval stage (with Df(3L)GN19)

(Vaskova et al., 2000)

decreased rate of movement | larval stage (with mge^A1)

(Vaskova et al., 2000)

decreased rate of movement | larval stage | recessive

(Vaskova et al., 2000)

lethal

(Colin et al., 2009)

lethal | recessive

(Harrison, 1999.5.12)

lethal - all die before end of larval stage (with Df(3L)449)

(Vaskova et al., 2000)

lethal - all die before end of larval stage (with Df(3L)GN19)

(Vaskova et al., 2000)

lethal - all die before end of larval stage (with mge^A1)

(Vaskova et al., 2000)

lethal - all die before end of larval stage | recessive

(Vaskova et al., 2000)

some die during larval stage (with Df(3L)449)

(Vaskova et al., 2000)

some die during larval stage (with Df(3L)GN19)

(Vaskova et al., 2000)

some die during larval stage (with mge^A1)

(Vaskova et al., 2000)

some die during larval stage | recessive

(Vaskova et al., 2000)

Phenotype Manifest In

Detailed Description

Statement

Reference

mge^B10 mutants do not display a wing phenotype.

(Colin et al., 2009)

Homozygous, mge^A1/mge^B10, mge^B10/Df(3L)449 and mge^B10/Df(3L)GN19 animals proceed through embryogenesis and hatch on schedule. However, the larvae do not grow but remain as first instars for 5-9 days (the normal first instar period is 24 hours). No gross morphological defects are seen, but mutant larvae appear to move more slowly than wild type.

(Vaskova et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

mge^B10/mge[+] is a suppressor of increased cell death phenotype of Mmus\Bax^UAS.cGa, Scer\GAL4^vg.PM

(Colin et al., 2009)

Phenotype Manifest In

Suppressor of

Statement

Reference

mge^B10/mge[+] is a suppressor of wing phenotype of Mmus\Bax^UAS.cGa, Scer\GAL4^vg.PM

(Colin et al., 2009)

mge^B10/mge[+] is a suppressor of wing margin phenotype of Mmus\Bax^UAS.cGa, Scer\GAL4^vg.PM

(Colin et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

A mge^B10 heterozygous background partially suppresses the wing phenotypes found upon expression of Mmus\Bax^Scer\UAS.cGa in the wing under the control of Scer\GAL4^vg.PM. A shift in phenotype distribution from about 60% of strong and about 40% of intermediate phenotypes in the control cross to 30% of strong and about 70% of intermediate phenotypes in these double mutants.

(Colin et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

5170

bw¹; mge^B10 st¹/TM6B, Tb⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

l(3)63Fc^B10

(Vaskova et al., 2000, Harrison, 1999.5.12)

mge^B10

mge^b10

(Colin et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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