FlyBase Allele Report: Dmel\Pak[4]

General Information

Symbol

Dmel\Pak⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0098344

Feature type

allele

Associated gene

Dmel\Pak

Associated Insertion(s)

Carried in Construct

Also Known As

dpak⁴

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Mutation abolishes dock binding.

(Hing et al., 1999)

Amino acid replacement: P9L.

(Hing et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:6,355,046..6,355,046 [+]

Nucleotide change:

C6355046T

Amino acid change:

Reported amino acid change:

P9L

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Hing et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with Pak⁶)

(Ang et al., 2006)

abnormal neuroanatomy | embryonic stage (with Pak^6-2)

(Kamiyama et al., 2015)

abnormal neuroanatomy | pupal stage (with Pak⁶)

(Ang et al., 2003)

lethal - all die before end of P-stage | recessive

(Hing et al., 1999)

some die during pharate adult stage | recessive

(Hing et al., 1999)

uncoordinated

(Hing et al., 1999)

Phenotype Manifest In

adult antennal nerve | P-stage (with Pak⁶)

(Ang et al., 2003)

antennal lobe (with Pak⁶)

(Ang et al., 2006)

dendrite | embryonic stage (with Pak^6-2)

(Kamiyama et al., 2015)

lamina

(Hing et al., 1999)

larval DA1 motor neuron | embryonic stage (with Pak^6-2)

(Kamiyama et al., 2015)

medulla

(Hing et al., 1999)

photoreceptor cell & axon

(Hing et al., 1999)

wing

(Hing et al., 1999)

Detailed Description

Statement

Reference

Pak^6-2/Pak⁴ transheterozygous embryos show defects in dendritogenesis in the embryonic aCC motoneurons (decreased number of dendritic tips) compared to controls.

(Kamiyama et al., 2015)

In Pak⁴/Pak⁶ mutants, the antennal lobes are smaller than those of wild type and the boundaries surrounding individual glomeruli are frequently missing.

(Ang et al., 2006)

In Pak⁶/Pak⁴ homozygous pupae 30 hours after puparium formation, the pattern of olfactory neuron projections from the antennae is normal. However, once in the antennal lobe, the axonal trajectories are clearly abnormal: instead of forming characteristic tracks, the fibers interweave to form a dense mat.

(Ang et al., 2003)

The ultrastructure of synapses in Pak¹¹/Pak⁴ mutant larvae is indistinguishable from wild type.

(Parnas et al., 2001)

Adult escapers are uncoordinated and have crumpled wings but are otherwise wild type in appearance. R cell axons extend into the brain normally. However these fibres do not spread evenly within the lamina and medulla. As a result some regions are hyperinnervated while others lack innervation. In the medulla neuropil, R cell axons fail to find their proper targets but instead terminate as abnormally thick, blunt ended fascicles. A small fraction of the R2-R5 neurons project through the lamina and into the medulla, indicating a modest disruption in ganglion target specificity. Some R1 to R6 axons fail to stop in the lamina, resulting in gaps in the lamina plexus. In addition, R cell axons form abnormally thick bundles between the lamina and medulla.

(Hing et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Pak⁴ is an enhancer of abnormal neuroanatomy phenotype of robo1⁵, sli¹

(Fan et al., 2003)

Suppressor of

Statement

Reference

Pak[+]/Pak⁴ is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of dbo^henji-1/dbo^henji-8

(Wang et al., 2016)

Pak⁴ is a suppressor | partially of visible phenotype of Scer\GAL4^en-e16E, tum^ΔE1E.UAS

(Sotillos and Campuzano, 2000)

Phenotype Manifest In

Enhancer of

Statement

Reference

Pak⁴ is an enhancer of presumptive embryonic/larval central nervous system phenotype of robo1⁵

(Fan et al., 2003)

Suppressor of

Statement

Reference

Pak[+]/Pak⁴ is a suppressor | partially of bouton | third instar larval stage phenotype of dbo^henji-1/dbo^henji-8

(Wang et al., 2016)

Pak[+]/Pak⁴ is a suppressor | partially of embryonic/larval neuromuscular junction | third instar larval stage phenotype of dbo^henji-1/dbo^henji-8

(Wang et al., 2016)

Pak⁴ is a suppressor | partially of wing vein phenotype of Scer\GAL4^en-e16E, tum^ΔE1E.UAS

(Sotillos and Campuzano, 2000)

Pak⁴ is a suppressor | partially of wing phenotype of Scer\GAL4^en-e16E, tum^ΔE1E.UAS

(Sotillos and Campuzano, 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Pak⁴/+ partially suppresses the increased satellite bouton phenotype seen in dbo^henji-1/dbo^henji-8 mutant larval neuromuscular junctions.

(Wang et al., 2016)

The antennal lobes of dock⁰⁴⁷²³/+; Pak⁴/+ animals exhibit normal axon trajectories and glomerular subdivisions.

(Ang et al., 2003)

Heterozygous Pak⁴ enhances the frequency of central nervous system axon defects seen in sli¹, robo⁵ mutants. An average of 6.1 defects are seen per animal. 54% of segments (calculated as number of defects/segments) show defects.

(Fan et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Pak⁴

(Ang et al., 2006)

dpak⁴

(Wang et al., 2016, Parnas et al., 2001)

pak1⁴

(Kamiyama et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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