FlyBase Allele Report: Dmel\dbo[henji-1]

General Information

Symbol

Dmel\dbo^henji-1

Species

D. melanogaster

Name

FlyBase ID

FBal0323169

Feature type

allele

Associated gene

Dmel\dbo

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

hypomorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Wang et al., 2016)

Mutagen

P-element activity

(Wang et al., 2016)

Progenitor genotype

P{GT1}DCP2^BG01766

(Wang et al., 2016)

Caused by aberration

Df(3L)dbo^henji-1

Cytology

Description

Deletion removing part of the 5'UTR of DCP2 and part of the 5'UTR of dbo.

(Wang et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage

(Wang et al., 2016)

abnormal neuroanatomy | third instar larval stage (with dbo^henji-8)

(Wang et al., 2016)

abnormal neurophysiology | third instar larval stage

(Wang et al., 2016)

abnormal neurophysiology | third instar larval stage (with dbo^c04604)

(Wang et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

dbo^henji-1/dbo^henji-1 and dbo^henji-1/dbo^henji-8 mutant larval neuromuscular junctions display an increase in satellite boutons (which are small boutons that emerge from large parental boutons). The number of boutons with normal size is slightly reduced in dbo^henji-1/dbo^henji-8 mutants, but is unchanged in dbo^henji-1/dbo^henji-1 mutants. dbo^henji-1/dbo^henji-8 mutants exhibit enlargement of the postsynaptic densities, as compared to controls.

dbo^henji-1/dbo^henji-1 mutant larval neuromuscular junctions display an expansion of the synaptic membrane region, as compared to controls.

dbo^henji-1/dbo^henji-1 and dbo^henji-1/dbo^c04604 mutant larval neuromuscular junctions do not show any difference in the amplitude of evoked junction potential (EJP), but do show strong elevation in miniature EJP (mEJP) amplitude, with no corresponding change in mEJP frequency, and significantly decreased quantal content, as compared to controls.

(Wang et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

dbo^henji-1/dbo^henji-8 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible by Pak[+]/Pak⁶

(Wang et al., 2016)

dbo^henji-1/dbo^henji-8 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by Pak[+]/Pak⁴

(Wang et al., 2016)

dbo^henji-1/dbo^henji-8 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by Pak[+]/Pak³

(Wang et al., 2016)

dbo^henji-1 has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by GluRIIA[+]/GluRIIA^unspecified

(Wang et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

dbo^henji-1/dbo^henji-8 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible | partially by Pak[+]/Pak³

(Wang et al., 2016)

dbo^henji-1 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible | partially by GluRIIA[+]/GluRIIA^unspecified

(Wang et al., 2016)

dbo^henji-1/dbo^henji-8 has bouton | third instar larval stage phenotype, suppressible by Pak[+]/Pak⁶

(Wang et al., 2016)

dbo^henji-1/dbo^henji-8 has bouton | third instar larval stage phenotype, suppressible | partially by Pak[+]/Pak⁴

(Wang et al., 2016)

dbo^henji-1/dbo^henji-8 has bouton | third instar larval stage phenotype, suppressible | partially by Pak[+]/Pak³

(Wang et al., 2016)

dbo^henji-1 has bouton | third instar larval stage phenotype, suppressible | partially by GluRIIA[+]/GluRIIA^unspecified

(Wang et al., 2016)

dbo^henji-1/dbo^henji-8 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible by Pak[+]/Pak⁶

(Wang et al., 2016)

dbo^henji-1/dbo^henji-8 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible | partially by Pak[+]/Pak⁴

(Wang et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Pak⁶/+ fully suppresses, and Pak⁴/+ or Pak³/+ partially suppresses the increased satellite bouton phenotype seen in dbo^henji-1/dbo^henji-8 mutant larval neuromuscular junctions.

GluRIIA^unspecified/+ partially suppresses the increased satellite bouton phenotype seen in dbo^henji-1/dbo^henji-1 mutant larval neuromuscular junctions.

(Wang et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

dbo^henji-1/dbo^henji-8 is rescued by dbo^GFP

(Wang et al., 2016)

dbo^c04604/dbo^henji-1 is rescued by dbo^UAS.GFP/Scer\GAL4^C57

(Wang et al., 2016)

dbo^henji-1 is rescued by dbo^UAS.GFP/Scer\GAL4^C57

(Wang et al., 2016)

Not rescued by

dbo^c04604/dbo^henji-1 is not rescued by Scer\GAL4^elav.PU/dbo^UAS.GFP

(Wang et al., 2016)

dbo^henji-1 is not rescued by Scer\GAL4^elav.PU/dbo^UAS.GFP

(Wang et al., 2016)

Comments

Expression of dbo^T:Avic\GFP rescues the enlarged postsynaptic density phenotype observed in dbo^henji-1/dbo^henji-8 mutants.

Expression of dbo^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^C57 (but not Scer\GAL4^elav.PU) rescues the increased number of satellite boutons seen in dbo^henji-1/dbo^henji-1 mutant larval neuromuscular junctions, and rescues the increased mEJP amplitude observed in dbo^henji-1/dbo^henji-1 and dbo^henji-1/dbo^c04604 mutant larval neuromuscular junctions.

(Wang et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

dbo^henji-1

henji¹

(Wang et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (1)

Report Sections

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