FlyBase Allele Report: Dmel\kn[UAS.cVa]

General Information

Symbol

Dmel\kn^UAS.cVa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Vervoort

FlyBase ID

FBal0098725

Feature type

allele

Associated gene

Dmel\kn

Associated Insertion(s)

Carried in Construct

P{UAS-kn.V}

Also Known As

UAS-col

Key Links

Transgenic product class

wild_type

(Vervoort et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Vervoort et al., 1999)

Mutagen

in vitro construct

(Vervoort et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-kn.V}

Cytology

Description

UAS regulatory sequences drive expression of kn.

(Vervoort et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | heat sensitive, with Scer\GAL4^srp.D.cCa

(Crozatier et al., 2004)

some die during embryonic stage | heat sensitive, with Scer\GAL4^srp.D.cCa

(Crozatier et al., 2004)

Phenotype Manifest In

Detailed Description

Statement

Reference

The LL1 muscle forms normally in Scer\GAL4^twi.PB, kn^Scer\UAS.cVa embryos. However, the DA2 muscle adopts a DA3 or intermediate DA3/DA2 morphology in 28% and 26% of segments, respectively, in these embryos.

(Enriquez et al., 2012)

Expression of kn^Scer\UAS.cVa under the control of Scer\GAL4^{kn-col85-GAL4} during lymph gland development results in an overproduction of posterior signaling center cells.

(Tokusumi et al., 2010)

Expression of kn^Scer\UAS.cVa under the control of Scer\GAL4^dome-PG125 results in an almost complete absence of haemocyte differentiation in the third larval instar lymph gland.

(Krzemień et al., 2007)

kn^Scer\UAS.cVa, Scer\GAL4^srp.D.cCa animals are viable when allowed to develop to the second instar larval stage at 18^oC before shifting to 25^oC. In a fraction of these larvae lamellocytes can be seen, a concomitant reduction in crystal cells is also seen. Around 5% of larvae develop melanotic tumours. No production of lamellocytes is seen when kn^Scer\UAS.cVa is expressed under the control of Scer\GAL4^lz-gal4 or Scer\GAL4^Hml.PG.

(Crozatier et al., 2004)

When driven by a variety of Scer\GAL4 drivers (Scer\GAL4^en-e16E, Scer\GAL4^ptc-559.1, Scer\GAL4^dpp.blk1 or Scer\GAL4^ap-md544) no detectable wing phenotype is seen.

(Vervoort et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^en-e16E/kn^UAS.cVa is an enhancer of visible | dominant phenotype of ci^D

(Vervoort et al., 1999)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^en-e16E/kn^UAS.cVa is an enhancer of wing vein L4 phenotype of ci^D

(Vervoort et al., 1999)

NOT Suppressor of

Statement

Reference

kn^UAS.cVa/Scer\GAL4^dpp.blk1 is a non-suppressor of first posterior wing cell phenotype of Scer\GAL4^dpp.blk1, ptc^UAS.cJa

(Vervoort et al., 1999)

Scer\GAL4^en-e16E/kn^UAS.cVa is a non-suppressor of first posterior wing cell phenotype of Scer\GAL4^en-e16E, ptc^UAS.cJa

(Vervoort et al., 1999)

kn^UAS.cVa/Scer\GAL4^dpp.blk1 is a non-suppressor of first posterior wing cell phenotype of Scer\GAL4^dpp.blk1, ci^76.UAS

(Vervoort et al., 1999)

kn^UAS.cVa/Scer\GAL4^unspecified is a non-suppressor of wing vein L3 phenotype of fu¹

(Vervoort et al., 1999)

kn^UAS.cVa/Scer\GAL4^unspecified is a non-suppressor of wing vein L4 phenotype of fu¹

(Vervoort et al., 1999)

Other

Statement

Reference

Scer\GAL4^how-24B, kn^UAS.cVa, nau^UAS.cKa has dorsal acute muscle cell | ectopic phenotype

(Enriquez et al., 2010)

Scer\GAL4^en-e16E, ci^D, kn^UAS.cVa has wing vein L5 phenotype

(Vervoort et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of kn^Scer\UAS.cVa and nau^Scer\UAS.cKa using Scer\GAL4^how-24B results in an ectopic DA3 muscle in thoracic segment 1 with very few nuclei (2-3).

(Enriquez et al., 2010)

When kn^Scer\UAS.cVa is driven by Scer\GAL4^en-e16E in a ci^D background, the truncation of L4 is stronger than seen in ci^D alone and L5 is partly missing. Overexpression of ptc^Scer\UAS.cJa driven by either Scer\GAL4^dpp.blk1 or Scer\GAL4^en-e16E, causes the partial loss of L3-L4 intervein. This phenotype cannot be rescued by Scer\GAL4 driven expression of kn^Scer\UAS.cVa. Expression of ci^76.Scer\UAS driven by Scer\GAL4^dpp.blk1 causes the partial loss of L3-L4 intervein. This phenotype cannot be rescued by Scer\GAL4 driven expression of kn^Scer\UAS.cVa.

(Vervoort et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

kn^UAS.cVa/Scer\GAL4^dpp.blk1 rescues kn¹/kn^col-1

(Vervoort et al., 1999)

Comments

When driven by Scer\GAL4^dpp.blk1, kn^Scer\UAS.cVa rescues the wing phenotype seen in kn¹/kn^col-1.

(Vervoort et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

kn^Scer\UAS.cVa

kn^UAS.cVa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Vervoort

(Vervoort et al., 1999)

Secondary FlyBase IDs

References (19)

Report Sections

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