FlyBase Allele Report: Dmel\N[cdc10.UAS.Tag:MYC]

General Information

Symbol

Dmel\N^{cdc10.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0104076

Feature type

allele

Associated gene

Dmel\N

Associated Insertion(s)

Carried in Construct

P{UAS-cdc10}

Also Known As

UAS-Notch^cdc10, UAS-N^act

Key Links

Transgenic product class

inframe_deletion

(Brennan et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Brennan et al., 1999)

Mutagen

in vitro construct

(Brennan et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-cdc10}

Cytology

Description

UAS sequences drive expression of an intracellular fragment of N, which encompasses the cdc10/ANK repeats. The first two amino acids of the cact protein followed by a 14 amino acid Tag:MYC epitope which is then fused to amino acids 1895--2156 of N.

(Brennan et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number, with Scer\GAL4^NP5130

(Montagne and Gonzalez-Gaitan, 2014)

decreased cell number | adult stage | somatic clone, with Scer\GAL4^Tub.PU

(Gervais et al., 2019, Andriatsilavo et al., 2018)

visible, with Scer\GAL4^ptc-559.1

(Brennan et al., 1999)

Phenotype Manifest In

adult midgut epithelium | somatic clone, with Scer\GAL4^Tub.PU

(Gervais et al., 2019, Andriatsilavo et al., 2018)

intestinal stem cell | adult stage | somatic clone, with Scer\GAL4^Tub.PU

(Gervais et al., 2019, Andriatsilavo et al., 2018)

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^NP5130

(Montagne and Gonzalez-Gaitan, 2014)

macrochaeta & postnotum, with Scer\GAL4^ptc-559.1

(Brennan et al., 1999)

Detailed Description

Statement

Reference

Somatic clones expressing N^{cdc10.UAS.Tag:MYC} under the control of Scer\GAL4^Tub.PU (in combination with a Gal80[ts] transgene to restrict expression to the adult stage) contain a smaller overall number of cells and intestinal stem cells in adult flies compared to controls.

(Gervais et al., 2019)

Adulthood-generated midgut clones expressing N^{cdc10.UAS.Tag:MYC} under the control of Scer\GAL4^Tub.PU have significantly fewer cells, namely intestinal stem cells, than control clones.

(Andriatsilavo et al., 2018)

The number of intestinal stem cells (ISCs) in the posterior midgut of flies expressing N^{cdc10.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^esg-NP5130 15 days after hatching is decreased compared with wild type.

(Montagne and Gonzalez-Gaitan, 2014)

Expression of N^{cdc10.Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^ptc-559.1 prevents the development of the scutellar bristles.

(Brennan et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

N^{cdc10.UAS.Tag:MYC}, Scer\GAL4^Tub.PU has increased cell number | somatic clone | adult stage phenotype, suppressible by spen^9C95/spen^9C95

(Andriatsilavo et al., 2018)

NOT suppressed by

Statement

Reference

N^{cdc10.UAS.Tag:MYC}, Scer\GAL4^NP5130 has decreased cell number phenotype, non-suppressible by Sara^GD8765, Scer\GAL4^NP5130

(Montagne and Gonzalez-Gaitan, 2014)

Suppressor of

Statement

Reference

Scer\GAL4^Tub.PU/N^{cdc10.UAS.Tag:MYC} is a suppressor of increased cell number | recessive | somatic clone | adult stage phenotype of kis^10D26

(Gervais et al., 2019)

Scer\GAL4^Tub.PU/N^{cdc10.UAS.Tag:MYC} is a suppressor of increased cell number | somatic clone | adult stage phenotype of spen^9C95

(Andriatsilavo et al., 2018)

N^{cdc10.UAS.Tag:MYC}, Scer\GAL4^NP5130 is a suppressor of increased cell number phenotype of Sara^GD8765, Scer\GAL4^NP5130

(Montagne and Gonzalez-Gaitan, 2014)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Tub.PU/N^{cdc10.UAS.Tag:MYC} is a non-suppressor of increased cell number | recessive | somatic clone | adult stage phenotype of kis^10D26

(Gervais et al., 2019)

Phenotype Manifest In

Suppressed by

Statement

Reference

N^{cdc10.UAS.Tag:MYC}, Scer\GAL4^Tub.PU has intestinal stem cell | somatic clone | adult stage phenotype, suppressible by spen^9C95/spen^9C95

(Andriatsilavo et al., 2018)

NOT suppressed by

Statement

Reference

N^{cdc10.UAS.Tag:MYC}, Scer\GAL4^NP5130 has intestinal stem cell of posterior adult midgut epithelium phenotype, non-suppressible by Sara^GD8765, Scer\GAL4^NP5130

(Montagne and Gonzalez-Gaitan, 2014)

Suppressor of

Statement

Reference

Scer\GAL4^Tub.PU/N^{cdc10.UAS.Tag:MYC} is a suppressor of intestinal stem cell | somatic clone phenotype of kis^10D26

(Gervais et al., 2019)

Scer\GAL4^Tub.PU/N^{cdc10.UAS.Tag:MYC} is a suppressor of intestinal stem cell | somatic clone | adult stage phenotype of spen^9C95

(Andriatsilavo et al., 2018)

Scer\GAL4^Tub.PU/N^{cdc10.UAS.Tag:MYC} is a suppressor of adult midgut epithelium | somatic clone phenotype of spen^9C95

(Andriatsilavo et al., 2018)

N^{cdc10.UAS.Tag:MYC}, Scer\GAL4^NP5130 is a suppressor of intestinal stem cell of posterior adult midgut epithelium phenotype of Sara^GD8765, Scer\GAL4^NP5130

(Montagne and Gonzalez-Gaitan, 2014)

Scer\GAL4^Tub.PU/N^{cdc10.UAS.Tag:MYC} is a suppressor of intestinal stem cell | increased number | somatic clone phenotype of Gmd^H78

(Perdigoto et al., 2011)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Tub.PU/N^{cdc10.UAS.Tag:MYC} is a non-suppressor of adult enterocyte | somatic clone phenotype of kis^10D26

(Gervais et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Adulthood-generated spen^9C95 midgut clones show a severe increase in the number of cells, namely of Intestinal stem cells (Dl-positive), as compared to control clones; both are suppressed if the clones express N^{cdc10.UAS.Tag:MYC} under the control of Scer\GAL4^Tub.PU. When N^{cdc10.UAS.Tag:MYC} expression is induced only 10 days after clone induction, the clones become composed of enterocytes.

(Andriatsilavo et al., 2018)

Flies co-expressing Sara^GD8765 and N^{cdc10.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^esg-NP5130 have a reduced number of intestinal stem cell compared to controls, as is seen when N^{cdc10.Scer\UAS.T:Hsap\MYC} is expressed alone.

(Montagne and Gonzalez-Gaitan, 2014)

Expression of N^{cdc10.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^tub.PU suppresses the formation of supernumerary intestinal stem cell-like cells which are produced in homozygous Gmd^H78 clones in the adult intestine.

(Perdigoto et al., 2011)

Expression of N^{cdc10.Scer\UAS.T:Hsap\MYC} driven by Scer\GAL4^ptc-559.1 has no effect on the Su(H)⁸/Su(H)² proneural cluster phenotype in the wing disc.

(Brennan et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

N^1895-2156

(Brennan et al., 1999)

N^{cdc10.Scer\UAS.T:Hsap\MYC}

N^{cdc10.UAS.Tag:MYC}

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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