FlyBase Allele Report: Dmel\Shc[111-40]

General Information

Symbol

Dmel\Shc^111-40

Species

D. melanogaster

Name

FlyBase ID

FBal0105105

Feature type

allele

Associated gene

Dmel\Shc

Associated Insertion(s)

Carried in Construct

Also Known As

dshc^111-40

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Luschnig et al., 2004)

Mutagen

ethyl methanesulfonate

(Luschnig et al., 2004, Luschnig et al., 2000)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C?T. Amino acid replacement: R152Y.

(Luschnig et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:9,427,655..9,427,655 [+]

Nucleotide change:

C9427655T

Reported nucleotide change:

C?T

Amino acid change:

R151W | Shc-PA

Reported amino acid change:

R151Y

Comment:

The mutation was reported as CGG to TGG, which is R to W, rather than R to Y as reported.

(Luschnig et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Mutant border cell clusters (induced as clones) migrate posteriorly, but do not migrate dorsally.

(Bianco et al., 2007)

Embryos that lack maternal Shc function (derived from females carrying homozygous germ line clones) have weak terminal defects. Embryos that lack both maternal and zygotic Shc function (derived from females carrying homozygous germ line clones mated to mutant males) have a weak flb-like phenotype. Homozygous adults have rough eyes.

(Luschnig et al., 2004)

When mutant somatic clones are made in the border cells no effect is seen.

(Duchek et al., 2001)

Embryos derived from homozygous female germ-line clones display terminal defects. Most embryos fail to hatch and show a reduction of posterior Filzkorper and the 8th abdominal segment as well as mild defects in the anterior head skeleton. Malpighian tubules are always absent. Embryos lacking both maternal and zygotic Shc function show defects in germband retraction and a variable reduction of ventral denticle belts not seen in paternally rescued embryos. 70% of homozygotes die during the late pupal stage. Females that survive to adulthood do not lay eggs.

(Luschnig et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Shc^111-40/Shc[+] is a suppressor of visible | semidominant phenotype of Egfr^E3

(Luschnig et al., 2000)

Shc^111-40/Shc^111-40 is a suppressor of visible phenotype of Scer\GAL4^CY2, btl::Egfr^λ.UAS

(Luschnig et al., 2000)

NOT Suppressor of

Statement

Reference

Shc^111-40 is a non-suppressor of visible phenotype of sev^S11.Tag:MYC

(Luschnig et al., 2000)

Other

Statement

Reference

Egfr^E3, Shc^111-40 has viable phenotype

(Luschnig et al., 2000)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Shc^111-40 is a non-enhancer of phenotype of Ras85D^V12.sev

(Luschnig et al., 2000)

Shc^111-40 is a non-enhancer of phenotype of rl^Sem

(Luschnig et al., 2000)

Shc^111-40 is a non-enhancer of phenotype of Raf::tor^12D.hs.sev

(Luschnig et al., 2000)

Suppressor of

Statement

Reference

Shc^111-40/Shc[+] is a suppressor of eye phenotype of Egfr^E3

(Luschnig et al., 2000)

Shc^111-40/Shc[+] is a suppressor of wing phenotype of Egfr^E3

(Luschnig et al., 2000)

Shc^111-40 is a suppressor of abdominal 11 anal tuft phenotype of tor^13D

(Luschnig et al., 2000)

Shc^111-40 is a suppressor of embryonic/first instar larval cuticle phenotype of tor^13D

(Luschnig et al., 2000)

Shc^111-40 is a suppressor of filzkorper phenotype of tor^13D

(Luschnig et al., 2000)

Shc^111-40/Shc^111-40 is a suppressor | partially of dorsal appendage phenotype of Scer\GAL4^CY2, btl::Egfr^λ.UAS

(Luschnig et al., 2000)

Shc^111-40/Shc^111-40 is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^CY2, btl::Egfr^λ.UAS

(Luschnig et al., 2000)

Shc^111-40/Shc^111-40 is a suppressor of wing phenotype of Scer\GAL4^CY2, btl::Egfr^λ.UAS

(Luschnig et al., 2000)

NOT Suppressor of

Statement

Reference

Shc^111-40 is a non-suppressor of phenotype of Ras85D^V12.sev

(Luschnig et al., 2000)

Shc^111-40 is a non-suppressor of phenotype of Raf::tor^12D.hs.sev

(Luschnig et al., 2000)

Shc^111-40 is a non-suppressor of phenotype of rl^Sem

(Luschnig et al., 2000)

Shc^111-40 is a non-suppressor of eye phenotype of sev^S11.Tag:MYC

(Luschnig et al., 2000)

Shc^111-40 is a non-suppressor of ommatidium phenotype of sev^S11.Tag:MYC

(Luschnig et al., 2000)

Other

Statement

Reference

Pvr^DN.UASp, Scer\GAL4^slbo.2.6, Shc^111-40 has border follicle cell | somatic clone phenotype

(Bianco et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Border cells clusters that are mutant for Shc^111-40 and also expressing Pvr^DN.Scer\UAS under the control of Scer\GAL4^slbo.2.6 show severely impaired posterior migration.

(Bianco et al., 2007)

Heterozygous females carrying tor^13D produce embryos that produce very little cuticle. This phenotype is weakly suppressed if the females are carrying one copy of Shc^111-40; the embryos develop more cuticle and show posterior structures such as Filzkorper and Tuft structures. The phenotype is completely suppressed if the tor^13D/+ females also carry homozygous Shc^111-40 germline clones; embryos look normal and can hatch. The eye and wing phenotypes caused by Egfr^E3 are dominantly suppressed by Shc^111-40. The Egfr^E3 phenotype is completely suppressed if the flies are also homozygous for Shc^111-40 and the viability of these flies is restored. Expression of btl::Egfr^{Scer\UAS.T:λ\cI-DD} under the control of Scer\GAL4^CY2 results in the formation of ectopic wing vein material at multiple sites on the wing. This phenotype is completely suppressed if the flies are also homozygous for Shc^111-40. Females expressing btl::Egfr^{Scer\UAS.T:λ\cI-DD} under the control of Scer\GAL4^CY2 produce strongly dorsalised eggs with no dorsal appendages. This phenotype is partially suppressed if the females are also homozygous for Shc^111-40; dorsal appendage material is produced around the anterior circumference of the egg.

(Luschnig et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a mutation in which embryos derived from homozygous germ-line clones show patterning defects in the cuticle.

(Luschnig et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

3L-111-40

(Luschnig et al., 2004)

Shc^111-40

(Bianco et al., 2007)

Shc^111/40

(Duchek et al., 2001)

dshc^111-40

(Wilson et al., 2005, Settle et al., 2003, Luschnig et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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