FlyBase Allele Report: Dmel\trio[GEF1.UAS.Tag:MYC]

General Information

Symbol

Dmel\trio^{GEF1.UAS.Tag:MYC}

Species

D. melanogaster

Name

FlyBase ID

FBal0117293

Feature type

allele

Associated gene

Dmel\trio

Associated Insertion(s)

Carried in Construct

P{UAS-trio.GEF1}

Key Links

Transgenic product class

inframe_deletion

(Newsome et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Newsome et al., 2000)

Mutagen

in vitro construct

(Newsome et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-trio.GEF1}

Cytology

Description

UAS regulatory sequences drive expression of the trio GEF1 domain, tagged at the 3' end with 8 copies of the Tag:MYC tag.

(Newsome et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

trio

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

abnormal neuroanatomy | adult stage, with Scer\GAL4^ey-OK107

(Lancaster et al., 2024)

abnormal neuroanatomy | larval stage, with Scer\GAL4^ppk.PG

(Lancaster et al., 2024)

lethal, with Scer\GAL4^c305a

(Lancaster et al., 2024)

partially lethal - majority die, with Scer\GAL4^ey-OK107

(Lancaster et al., 2024)

Phenotype Manifest In

adult mushroom body, with Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

adult mushroom body alpha-lobe | absent, with Scer\GAL4^ey-OK107

(Lancaster et al., 2024)

adult mushroom body beta-lobe | absent, with Scer\GAL4^ey-OK107

(Lancaster et al., 2024)

dendritic branch | larval stage, with Scer\GAL4^ppk.PG

(Lancaster et al., 2024)

larval dorsal multidendritic neuron ddaC, with Scer\GAL4^ppk.PG

(Lancaster et al., 2024)

lobe system of adult mushroom body, with Scer\GAL4^ey-OK107

(Lancaster et al., 2024)

photoreceptor cell & axon, with Scer\GAL4^GMR.PF

(Newsome et al., 2000)

photoreceptor cell & eye disc, with Scer\GAL4^GMR.PF

(Newsome et al., 2000)

photoreceptor neuron, with Scer\GAL4^GMR.PF

(Newsome et al., 2000)

Detailed Description

Statement

Reference

Expression of trio^{GEF1.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^OK107 results in severe axon growth defects in the mushroom body.

(Ng and Luo, 2004)

Expression of trio^{GEF1.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^GMR.PF causes moderate defects in photoreceptor axon projection. Many axons appear to clump together in the optic stalk, while those that reach the brain project in an extremely disorganised fashion into the optic lobe or sometimes even grow instead over the surface of the brain. The pattern of photoreceptor clusters in the eye disc is also severely disrupted and many photoreceptors delaminate and migrate into the optic stalk.

(Newsome et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^ey-OK107/trio^{GEF1.UAS.Tag:MYC} is an enhancer of increased mortality during development phenotype of Nab2^ex3

(Lancaster et al., 2024)

Scer\GAL4^15J2/trio^{GEF1.UAS.Tag:MYC} is an enhancer of abnormal neuroanatomy | recessive phenotype of N^l1N-ts1