FlyBase Allele Report: Dmel\Nab2[ex3]

Nab2^ex3 homozygous adults exhibit highly penetrant structural defects in the mushroom body: thinning or absence of the dorsally projecting α lobes and overprojection or fusion of the medially projecting β lobes.

(Rounds et al., 2022)

Nab2^ex3 homozygous adults frequently exhibit mushroom body morphology defects including missing or thinned mushroom body α-lobes and β-lobe fusion.

(Bienkowski et al., 2017)

Nab2^ex3/Nab2^ex3 adult brains have thinned or missing mushroom body alpha- and beta- lobes and overgrowth of beta-lobes across the midline (but no effect on gamma-lobes); mutants also have ellipsoid body defects (lack a completely closed ring structure, with an opening left on the ventral side). Beta-lobe defects occur in around 80% and alpha-lobe defects occur in around 65% of Nab2^ex3/Nab2^ex3 brains (compared to less than 10% in controls). Many Nab2^ex3/Nab2^ex3 brains show beta-lobe fusion and beta-lobe axons cross the midline; Nab2^ex3/Nab2^ex3 impairs both projection and branching of developing MB axons. Individual mushroom body beta-lobe Nab2^ex3/Nab2^ex3 neuron axons project medially but fail to stop, forming a meshed network of axons across the brain midline.

(Kelly et al., 2016)

Only a small percentage (~3%) of homozygous Nab2^ex3 flies eclose as adults.

While 97% of Nab2^ex3 homozygotes die as pharate adults, the 3% than eclose display a variety of organismal phenotypes including impaired locomotor behavior, fully penetrant kinking of thoracic bristles, and a wings-held-out posture.

Nab2^ex3 homozygous mutants exhibit severely reduced locomotion compared to wild-type flies.

Nab2^ex3 mutant flies exhibit extended poly(A) tails compared to wild-type.

(Kelly et al., 2014)

The majority of homozygotes and Nab2^ex3/Df(3R)Exel8178 animals die at the pharate adult stage, often as partially emerged adults. 3-5% of homozygotes emerge completely, but they have a shortened lifespan (approximately 1.5 weeks). These adult escapers also show a held-out wings phenotype, bending of the major thoracic bristles and disorganisation of the minor thoracic bristles. Adult escapers show severely compromised flight behaviour and poor locomotor activity.

Embryos lacking germline Nab2 function die early in embryogenesis.

(Pak et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Nab2^ex3 has increased mortality during development phenotype, enhanceable by Scer\GAL4^ey-OK107/trio^{GEF1.UAS.Tag:MYC}

(Lancaster et al., 2024)

Nab2^ex3 has abnormal neuroanatomy | adult stage phenotype, enhanceable by Fmr1^Δ50M/Fmr1[+]

(Bienkowski et al., 2017)

Nab2^ex3 has abnormal neuroanatomy | adult stage phenotype, enhanceable by Scer\GAL4^ey-OK107/Fmr1^UAS.cWa

(Bienkowski et al., 2017)

NOT Enhanced by

Statement

Reference

Nab2^ex3 has short lived phenotype, non-enhanceable by Scer\GAL4^ey-OK107/trio^{GEF2.UAS.Tag:MYC}

(Lancaster et al., 2024)

Nab2^ex3 has partially lethal phenotype, non-enhanceable by Wnt4^C1/Wnt4[+]

(Lee et al., 2020)

Nab2^ex3 has some die during P-stage phenotype, non-enhanceable by Wnt4^C1/Wnt4[+]

(Lee et al., 2020)

Nab2^ex3 has partially lethal phenotype, non-enhanceable by pk[+]/pk^pk-sple-14

(Lee et al., 2020)

Nab2^ex3 has some die during P-stage phenotype, non-enhanceable by pk[+]/pk^pk-sple-14

(Lee et al., 2020)

Suppressed by

Statement

Reference

Nab2^ex3 has increased mortality during development phenotype, suppressible | partially by Ythdc1^ΔN/Ythdc1^ΔN

(Lancaster et al., 2024)

Nab2^ex3 has abnormal neuroanatomy | larval stage phenotype, suppressible by Scer\GAL4^ppk.PG/trio^{GEF2.UAS.Tag:MYC}

(Lancaster et al., 2024)

Nab2^ex3 has increased mortality during development phenotype, suppressible | partially by Scer\GAL4^ey-OK107/trio^{GEF2.UAS.Tag:MYC}

(Lancaster et al., 2024)

Nab2^ex3 has decreased rate of adult locomotory behavior phenotype, suppressible | partially by Scer\GAL4^ey-OK107/trio^{GEF2.UAS.Tag:MYC}

(Lancaster et al., 2024)

Nab2^ex3 has abnormal neuroanatomy | adult stage phenotype, suppressible | partially by Scer\GAL4^c305a/trio^{GEF2.UAS.Tag:MYC}

(Lancaster et al., 2024)

Nab2^ex3 has increased mortality during development phenotype, suppressible | partially by Atx2^X1/Atx2[+]

(Rounds et al., 2022)

Nab2^ex3 has partially lethal - majority die phenotype, suppressible | male limited | female limited | partially by Atx2^DG08112/Atx2[+]

(Rounds et al., 2022)

Nab2^ex3 has abnormal neuroanatomy | adult stage phenotype, suppressible | partially by Atx2^X1/Atx2[+]

(Rounds et al., 2022)

Nab2^ex3 has abnormal neuroanatomy | adult stage phenotype, suppressible | partially by Atx2^DG08112/Atx2[+]

(Rounds et al., 2022)

Nab2^ex3 has increased mortality during development phenotype, suppressible | partially by Appl^d/Appl[+]

(Lee et al., 2020)

Nab2^ex3 has increased mortality during development phenotype, suppressible | partially by dsh¹/dsh[+]

(Lee et al., 2020)

Nab2^ex3 has majority die during pharate adult stage phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\ZC3H14^{Iso1.UAS.Tag:FLAG}

(Kelly et al., 2014)

Nab2^ex3 has abnormal locomotor behavior phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\ZC3H14^{Iso1.UAS.Tag:FLAG}

(Kelly et al., 2014)

NOT suppressed by

Statement

Reference

Nab2^ex3 has partially lethal - majority die phenotype, non-suppressible by Ythdf⁰/Ythdf[+]

(Lancaster et al., 2024)

Nab2^ex3 has short lived phenotype, non-suppressible by Ythdc1^ΔN/Ythdc1^ΔN

(Lancaster et al., 2024)

Nab2^ex3 has short lived phenotype, non-suppressible by Ythdf⁰/Ythdf[+]

(Lancaster et al., 2024)

Nab2^ex3 has short lived phenotype, non-suppressible by Scer\GAL4^ey-OK107/trio^{GEF2.UAS.Tag:MYC}

(Lancaster et al., 2024)

Nab2^ex3 has partially lethal - majority die phenotype, non-suppressible | male limited | female limited | partially by Atx2^DG08112/Atx2[+]

(Rounds et al., 2022)

Nab2^ex3 has partially lethal phenotype, non-suppressible by pk[+]/pk^pk-sple-14

(Lee et al., 2020)

Nab2^ex3 has partially lethal phenotype, non-suppressible by Wnt4^C1/Wnt4[+]

(Lee et al., 2020)

Nab2^ex3 has some die during P-stage phenotype, non-suppressible by pk[+]/pk^pk-sple-14

(Lee et al., 2020)

Nab2^ex3 has some die during P-stage phenotype, non-suppressible by Wnt4^C1/Wnt4[+]

(Lee et al., 2020)

Nab2^ex3 has majority die during pharate adult stage phenotype, non-suppressible by Scer\GAL4^elav-C155/Hsap\ZC3H14^{Iso1.UAS.Tag:FLAG}

(Kelly et al., 2014)

Nab2^ex3 has abnormal locomotor behavior phenotype, non-suppressible by Scer\GAL4^elav-C155/Hsap\ZC3H14^{Iso1.UAS.Tag:FLAG}

(Kelly et al., 2014)

Enhancer of

Statement

Reference

Nab2^ex3/Nab2[+] is an enhancer of abnormal memory | adult stage phenotype of Fmr1^Δ50M

(Bienkowski et al., 2017)

Suppressor of

Statement

Reference

Nab2^ex3/Nab2[+] is a suppressor of abnormal neuroanatomy | adult stage phenotype of Fmr1^Δ50M

(Bienkowski et al., 2017)

Other

Statement

Reference

Nab2^ex3, Scer\GAL4^ey-OK107, trio^{GEF1.UAS.Tag:MYC} has lethal phenotype

(Lancaster et al., 2024)

Nab2^ex3, Scer\GAL4^c305a, trio^{GEF1.UAS.Tag:MYC} has lethal phenotype

(Lancaster et al., 2024)

Nab2^ex3, Ythdc1^ΔN has partially lethal - majority die phenotype

(Lancaster et al., 2024)

Nab2^ex3, Ythdf⁰ has lethal phenotype

(Lancaster et al., 2024)

Atx2^X1/Atx2[+], Nab2^ex3 has partially lethal - majority live phenotype

(Rounds et al., 2022)

Phenotype Manifest In

Enhanced by

Statement

Reference

Nab2^ex3 has adult mushroom body alpha-lobe | adult stage phenotype, enhanceable by Fmr1^Δ50M/Fmr1[+]

(Bienkowski et al., 2017)

Nab2^ex3 has mushroom body | adult stage phenotype, enhanceable by Scer\GAL4^ey-OK107/Fmr1^UAS.cWa

(Bienkowski et al., 2017)

NOT Enhanced by

Statement

Reference

Nab2^ex3 has adult mushroom body beta-lobe | adult stage phenotype, non-enhanceable by Fmr1^Δ50M/Fmr1[+]

(Bienkowski et al., 2017)

Suppressed by

Statement

Reference

Nab2^ex3 has adult mushroom body alpha-lobe phenotype, suppressible by Scer\GAL4^ey-OK107/trio^{GEF2.UAS.Tag:MYC}

(Lancaster et al., 2024)

Nab2^ex3 has adult mushroom body alpha'-lobe phenotype, suppressible by Scer\GAL4^c305a/trio^{GEF2.UAS.Tag:MYC}

(Lancaster et al., 2024)

Nab2^ex3 has adult mushroom body beta'-lobe phenotype, suppressible | partially by Scer\GAL4^c305a/trio^{GEF2.UAS.Tag:MYC}

(Lancaster et al., 2024)

Nab2^ex3 has larval dorsal multidendritic neuron ddaC phenotype, suppressible by Scer\GAL4^ppk.PG/trio^{GEF2.UAS.Tag:MYC}

(Lancaster et al., 2024)

Nab2^ex3 has dendritic branch | larval stage phenotype, suppressible by Scer\GAL4^ppk.PG/trio^{GEF2.UAS.Tag:MYC}

(Lancaster et al., 2024)

Nab2^ex3 has adult mushroom body alpha-lobe phenotype, suppressible | partially by Atx2^DG08112/Atx2[+]

(Rounds et al., 2022)

Nab2^ex3 has axon | adult stage phenotype, suppressible | partially by Atx2^DG08112/Atx2[+]

(Rounds et al., 2022)

Nab2^ex3 has adult mushroom body alpha-lobe phenotype, suppressible | partially by Atx2^X1/Atx2[+]

(Rounds et al., 2022)

Nab2^ex3 has axon | adult stage phenotype, suppressible | partially by Atx2^X1/Atx2[+]

(Rounds et al., 2022)

Nab2^ex3 has humeral bristle phenotype, suppressible | partially by dsh¹/dsh[+]

(Lee et al., 2020)

Nab2^ex3 has wing phenotype, suppressible by Scer\GAL4^elav-C155/Hsap\ZC3H14^{Iso1.UAS.Tag:FLAG}

(Kelly et al., 2014)

NOT suppressed by

Statement

Reference

Nab2^ex3 has adult mushroom body beta-lobe phenotype, non-suppressible by Scer\GAL4^ey-OK107/trio^{GEF2.UAS.Tag:MYC}

(Lancaster et al., 2024)

Nab2^ex3 has adult mushroom body beta-lobe phenotype, non-suppressible by Atx2^X1/Atx2[+]

(Rounds et al., 2022)

Nab2^ex3 has adult mushroom body beta-lobe phenotype, non-suppressible by Atx2^DG08112/Atx2[+]

(Rounds et al., 2022)

Nab2^ex3 has wing phenotype, non-suppressible by Scer\GAL4^elav-C155/Hsap\ZC3H14^{Iso1.UAS.Tag:FLAG}

(Kelly et al., 2014)

Nab2^ex3 has macrochaeta phenotype, non-suppressible by Scer\GAL4^elav-C155/Hsap\ZC3H14^{Iso1.UAS.Tag:FLAG}

(Kelly et al., 2014)

Suppressor of

Statement

Reference

Nab2^ex3/Nab2[+] is a suppressor of adult mushroom body alpha-lobe | adult stage phenotype of Fmr1^Δ50M

(Bienkowski et al., 2017)

NOT Suppressor of

Statement

Reference

Nab2^ex3/Nab2[+] is a non-suppressor of adult mushroom body beta-lobe | adult stage phenotype of Fmr1^Δ50M

(Bienkowski et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The mushroom body α-lobe morphological defects characteristic for Nab2^ex3 homozygous adults are worsened by combination with a single copy of Fmr1^Δ50M or Fmr1^Δ113M, whereas the reverse is true for either Fmr1^Δ50M or Fmr1^Δ113M homozygotes in which the combination with one copy of Nab2^ex3 ameliorates the α-lobe phenotype. The β-lobe fusion observed in any of the homozygous mutants cannot be modified by heterozygosity of the other gene's allele. The mushroom body defects in Nab2^ex3 mutants are also exacerbated by Scer\GAL4^ey-OK107-driven expression of Fmr1^Scer\UAS.cWa.

The mild memory defects displayed by the Fmr1^Δ50M heterozygotes in an aversive olfactory conditioning assay are enhanced by Nab2^ex3 heterozygosity.

(Bienkowski et al., 2017)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\ZC3H14^{Iso1.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^elav-C155 rescues the eclosion rate of Nab2^ex3 animals from ~3% to ~78%. The presence of Hsap\ZC3H14^{Iso1.Scer\UAS.T:Zzzz\FLAG} alone (i.e. without a Scer\GAL4 driver) increases the eclosion rate to 19%. Neuronal expression of Hsap\ZC3H14^{Iso1.Scer\UAS.T:Zzzz\FLAG} restores normal wing posture in Nab2^ex3 animals. Thoracic bristle kinking is not rescued by pan-neuronal expression of Hsap\ZC3H14^{Iso1.Scer\UAS.T:Zzzz\FLAG}.

Neuron-specific expression of Hsap\ZC3H14^{Iso1.Scer\UAS.T:Zzzz\FLAG} (under the control of Scer\GAL4^elav-C155) significantly rescues the severely reduced locomotion in Nab2^ex3 homozygous mutants.

Expression of Hsap\ZC3H14^{Iso1.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^elav-C155 removes the extended neuronal RNA poly(A) tails found in Nab2^ex3 mutant flies.

Expression of Hsap\ZC3H14^{Iso4.Scer\UAS.T:Zzzz\FLAG} in Nab2^ex3 homozygotes, under the control of Scer\GAL4^elav-C155, does not improve eclosion rates. Expression does not rescue the wing-held-out posturing, thoracic bristle kinking and locomotion defects found in Nab2^ex3 homozygotes.

Expression of Hsap\ZC3H14^{Iso1.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^elav-C155 fails to remove the extended neuronal RNA poly(A) tails found in Nab2^ex3 mutant flies.

(Kelly et al., 2014)

Complementation and Rescue Data

Rescued by

Nab2^ex3 is rescued by Scer\GAL4^elav-C155/Nab2^{fl.UAS.Tag:FLAG}

(Kelly et al., 2014)

Nab2^ex3 is rescued by Nab2^{fl.UAS.Tag:FLAG}

(Kelly et al., 2014)

Nab2^ex3 is rescued by Scer\GAL4^elav.PU/Nab2^UAS.Tag:FLAG

(Pak et al., 2011)

Partially rescued by

Nab2^ex3 is partially rescued by Scer\GAL4^elav-C155/Nab2^UAS.Tag:FLAG

(Kelly et al., 2016)

Nab2^ex3 is partially rescued by Scer\GAL4^ey-OK107/Nab2^UAS.Tag:FLAG

(Kelly et al., 2016)

Nab2^ex3 is partially rescued by Nab2^UAS.Tag:FLAG

(Pak et al., 2011)

Not rescued by

Nab2^ex3 is not rescued by Nab2^UAS.Tag:FLAG/Scer\GAL4^c739

(Kelly et al., 2016)

Nab2^ex3 is not rescued by Scer\GAL4^Tab2-201Y/Nab2^UAS.Tag:FLAG

(Kelly et al., 2016)

Nab2^ex3 is not rescued by Scer\GAL4^c305a/Nab2^UAS.Tag:FLAG

(Kelly et al., 2016)

Comments

Expression of Nab2^{Scer\UAS.T:Zzzz\FLAG} driven by Scer\GAL4^elav-C155 or Scer\GAL4^ey-OK107 (but not Scer\GAL4^c739, Scer\GAL4^Tab2-201Y or Scer\GAL4^c305a) significantly rescues mushroom body beta-lobe fusion defects in most Nab2^ex3/Nab2^ex3 brains.

(Kelly et al., 2016)

Expression of Nab2^{fl.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^elav-C155 rescues eclosion rates in Nab2^ex3 mutants from ~3% to 93%. The presence of Nab2^{fl.Scer\UAS.T:Zzzz\FLAG} alone (i.e. without a Scer\GAL4 driver) increases eclosion rates from 3% to 39% in Nab2^ex3 homozygous mutants.

Expression of Nab2^{fl.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^elav-C155 restores normal wing posture in eclosed Nab2^ex3 homozygous mutants. Thoracic bristle kinking is not rescued by pan-neuronal expression of Nab2^{fl.Scer\UAS.T:Zzzz\FLAG}.

Expression of Nab2^{fl.Scer\UAS.T:Zzzz\FLAG} under the control of Scer\GAL4^elav-C155 removes the extended neuronal RNA poly(A) tails found in Nab2^ex3 mutant flies.

(Kelly et al., 2014)

Nab2^{Scer\UAS.T:Zzzz\FLAG} alone (in the absence of a Scer\GAL4 driver) partially rescues the eclosion and locomotor defects of Nab2^ex3 mutants. When expression is driven by Scer\GAL4^elav.PU, full rescue of the mutant phenotypes is seen.

(Pak et al., 2011)

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Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Nab2^ex3

(Lancaster et al., 2024, Corgiat et al., 2022, Corgiat et al., 2021, Lee et al., 2020, Kelly et al., 2014)

Nab2^null

(Lancaster et al., 2024, Kelly et al., 2014)

dNab2^ex3

(Bienkowski et al., 2017, Kelly et al., 2016)

ex3

(Pak et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (9)

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