The mushroom body α-lobe morphological defects characteristic for Nab2ex3 homozygous adults are worsened by combination with a single copy of Fmr1Δ50M or Fmr1Δ113M, whereas the reverse is true for either Fmr1Δ50M or Fmr1Δ113M homozygotes in which the combination with one copy of Nab2ex3 ameliorates the α-lobe phenotype. The β-lobe fusion observed in any of the homozygous mutants cannot be modified by heterozygosity of the other gene's allele. The mushroom body defects in Nab2ex3 mutants are also exacerbated by Scer\GAL4ey-OK107-driven expression of Fmr1Scer\UAS.cWa.
Expression of Hsap\ZC3H14Iso1.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 rescues the eclosion rate of Nab2ex3 animals from ~3% to ~78%. The presence of Hsap\ZC3H14Iso1.Scer\UAS.T:Zzzz\FLAG alone (i.e. without a Scer\GAL4 driver) increases the eclosion rate to 19%. Neuronal expression of Hsap\ZC3H14Iso1.Scer\UAS.T:Zzzz\FLAG restores normal wing posture in Nab2ex3 animals. Thoracic bristle kinking is not rescued by pan-neuronal expression of Hsap\ZC3H14Iso1.Scer\UAS.T:Zzzz\FLAG.
Expression of Hsap\ZC3H14Iso4.Scer\UAS.T:Zzzz\FLAG in Nab2ex3 homozygotes, under the control of Scer\GAL4elav-C155, does not improve eclosion rates. Expression does not rescue the wing-held-out posturing, thoracic bristle kinking and locomotion defects found in Nab2ex3 homozygotes.
Expression of Nab2Scer\UAS.T:Zzzz\FLAG driven by Scer\GAL4elav-C155 or Scer\GAL4ey-OK107 (but not Scer\GAL4c739, Scer\GAL4Tab2-201Y or Scer\GAL4c305a) significantly rescues mushroom body beta-lobe fusion defects in most Nab2ex3/Nab2ex3 brains.
Expression of Nab2fl.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 rescues eclosion rates in Nab2ex3 mutants from ~3% to 93%. The presence of Nab2fl.Scer\UAS.T:Zzzz\FLAG alone (i.e. without a Scer\GAL4 driver) increases eclosion rates from 3% to 39% in Nab2ex3 homozygous mutants.
Expression of Nab2fl.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 restores normal wing posture in eclosed Nab2ex3 homozygous mutants. Thoracic bristle kinking is not rescued by pan-neuronal expression of Nab2fl.Scer\UAS.T:Zzzz\FLAG.