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General Information
Symbol
Dmel\Nab2ex3
Species
D. melanogaster
Name
FlyBase ID
FBal0265606
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dNab2ex3
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the progenitor insertion, resulting in a 1.5kb deletion that extends into the Nab2 gene.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Nab2ex3 homozygous adults frequently exhibit mushroom body morphology defects including missing or thinned mushroom body α-lobes and β-lobe fusion.

Nab2ex3/Nab2ex3 adult brains have thinned or missing mushroom body alpha- and beta- lobes and overgrowth of beta-lobes across the midline (but no effect on gamma-lobes); mutants also have ellipsoid body defects (lack a completely closed ring structure, with an opening left on the ventral side). Beta-lobe defects occur in around 80% and alpha-lobe defects occur in around 65% of Nab2ex3/Nab2ex3 brains (compared to less than 10% in controls). Many Nab2ex3/Nab2ex3 brains show beta-lobe fusion and beta-lobe axons cross the midline; Nab2ex3/Nab2ex3 impairs both projection and branching of developing MB axons. Individual mushroom body beta-lobe Nab2ex3/Nab2ex3 neuron axons project medially but fail to stop, forming a meshed network of axons across the brain midline.

Only a small percentage (~3%) of homozygous Nab2ex3 flies eclose as adults.

While 97% of Nab2ex3 homozygotes die as pharate adults, the 3% than eclose display a variety of organismal phenotypes including impaired locomotor behavior, fully penetrant kinking of thoracic bristles, and a wings-held-out posture.

Nab2ex3 homozygous mutants exhibit severely reduced locomotion compared to wild-type flies.

Nab2ex3 mutant flies exhibit extended poly(A) tails compared to wild-type.

The majority of homozygotes and Nab2ex3/Df(3R)Exel8178 animals die at the pharate adult stage, often as partially emerged adults. 3-5% of homozygotes emerge completely, but they have a shortened lifespan (approximately 1.5 weeks). These adult escapers also show a held-out wings phenotype, bending of the major thoracic bristles and disorganisation of the minor thoracic bristles. Adult escapers show severely compromised flight behaviour and poor locomotor activity.

Embryos lacking germline Nab2 function die early in embryogenesis.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Statement
Reference

Nab2ex3/Nab2[+] is an enhancer of memory defective | adult stage phenotype of Fmr1Δ50M

Suppressor of
Statement
Reference
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Statement
Reference

Nab2ex3 has mushroom body beta-lobe | adult stage phenotype, non-enhanceable by Fmr1Δ50M/Fmr1[+]

Suppressed by
Statement
Reference
NOT suppressed by
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

Nab2ex3/Nab2[+] is a non-suppressor of mushroom body beta-lobe | adult stage phenotype of Fmr1Δ50M

Additional Comments
Genetic Interactions
Statement
Reference

The mushroom body α-lobe morphological defects characteristic for Nab2ex3 homozygous adults are worsened by combination with a single copy of Fmr1Δ50M or Fmr1Δ113M, whereas the reverse is true for either Fmr1Δ50M or Fmr1Δ113M homozygotes in which the combination with one copy of Nab2ex3 ameliorates the α-lobe phenotype. The β-lobe fusion observed in any of the homozygous mutants cannot be modified by heterozygosity of the other gene's allele. The mushroom body defects in Nab2ex3 mutants are also exacerbated by Scer\GAL4ey-OK107-driven expression of Fmr1Scer\UAS.cWa.

The mild memory defects displayed by the Fmr1Δ50M heterozygotes in an aversive olfactory conditioning assay are enhanced by Nab2ex3 heterozygosity.

Xenogenetic Interactions
Statement
Reference

Expression of Hsap\ZC3H14Iso1.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 rescues the eclosion rate of Nab2ex3 animals from ~3% to ~78%. The presence of Hsap\ZC3H14Iso1.Scer\UAS.T:Zzzz\FLAG alone (i.e. without a Scer\GAL4 driver) increases the eclosion rate to 19%. Neuronal expression of Hsap\ZC3H14Iso1.Scer\UAS.T:Zzzz\FLAG restores normal wing posture in Nab2ex3 animals. Thoracic bristle kinking is not rescued by pan-neuronal expression of Hsap\ZC3H14Iso1.Scer\UAS.T:Zzzz\FLAG.

Neuron-specific expression of Hsap\ZC3H14Iso1.Scer\UAS.T:Zzzz\FLAG (under the control of Scer\GAL4elav-C155) significantly rescues the severely reduced locomotion in Nab2ex3 homozygous mutants.

Expression of Hsap\ZC3H14Iso1.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 removes the extended neuronal RNA poly(A) tails found in Nab2ex3 mutant flies.

Expression of Hsap\ZC3H14Iso4.Scer\UAS.T:Zzzz\FLAG in Nab2ex3 homozygotes, under the control of Scer\GAL4elav-C155, does not improve eclosion rates. Expression does not rescue the wing-held-out posturing, thoracic bristle kinking and locomotion defects found in Nab2ex3 homozygotes.

Expression of Hsap\ZC3H14Iso1.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 fails to remove the extended neuronal RNA poly(A) tails found in Nab2ex3 mutant flies.

Complementation and Rescue Data
Comments

Expression of Nab2Scer\UAS.T:Zzzz\FLAG driven by Scer\GAL4elav-C155 or Scer\GAL4ey-OK107 (but not Scer\GAL4c739, Scer\GAL4Tab2-201Y or Scer\GAL4c305a) significantly rescues mushroom body beta-lobe fusion defects in most Nab2ex3/Nab2ex3 brains.

Expression of Nab2fl.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 rescues eclosion rates in Nab2ex3 mutants from ~3% to 93%. The presence of Nab2fl.Scer\UAS.T:Zzzz\FLAG alone (i.e. without a Scer\GAL4 driver) increases eclosion rates from 3% to 39% in Nab2ex3 homozygous mutants.

Expression of Nab2fl.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 restores normal wing posture in eclosed Nab2ex3 homozygous mutants. Thoracic bristle kinking is not rescued by pan-neuronal expression of Nab2fl.Scer\UAS.T:Zzzz\FLAG.

Expression of Nab2fl.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4elav-C155 removes the extended neuronal RNA poly(A) tails found in Nab2ex3 mutant flies.

Nab2Scer\UAS.T:Zzzz\FLAG alone (in the absence of a Scer\GAL4 driver) partially rescues the eclosion and locomotor defects of Nab2ex3 mutants. When expression is driven by Scer\GAL4elav.PU, full rescue of the mutant phenotypes is seen.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (4)