FlyBase Allele Report: Dmel\Wnt4[C1]

General Information

Symbol

Dmel\Wnt4^C1

Species

D. melanogaster

Name

FlyBase ID

FBal0141321

Feature type

allele

Associated gene

Dmel\Wnt4

Associated Insertion(s)

Carried in Construct

Also Known As

DWnt4^C1

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

(Cohen et al., 2002)

Progenitor genotype

Cytology

Description

3bp deletion removes a highly conserved glutamate residue at position 299.

(Cohen et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:7,257,005..7,257,007 [-]

Comment:

Reported as a 3 bp deletion of gluatamate 299, however, the nearest E residue is at 302.

(Cohen et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates acute myeloid leukemia

modeled by Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa

(Sinenko et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with Wnt4^EMS23)

(Sato et al., 2006)

abnormal neuroanatomy | larval stage (with Wnt4^EMS23)

(Mieszczanek et al., 2022)

lethal (with Df(2L)BSC324)

(Kahsai and Cook, 2018)

partially lethal - majority die | recessive

(Cohen et al., 2002)

some die during larval stage | recessive

(Cohen et al., 2002)

sterile | recessive

(Cohen et al., 2002)

Phenotype Manifest In

axon | larval stage (with Wnt4^EMS23)

(Mieszczanek et al., 2022)

embryonic/larval optic stalk (with Wnt4^EMS23)

(Sato et al., 2006)

eye photoreceptor cell (with Wnt4^EMS23)

(Sato et al., 2006)

lamina (with Wnt4^EMS23)

(Sato et al., 2006)

larval brain | larval stage (with Wnt4^EMS23)

(Mieszczanek et al., 2022)

neuron (with Wnt4^EMS23)

(Sato et al., 2006)

ovariole

(Gupta and Schupbach, 2003)

Detailed Description

Statement

Reference

One copy of Wnt4^C1 has no effect on hemocyte proliferation in third instar larvae.

(Sinenko et al., 2010)

Wnt4^C1/Wnt4^EMS23 mutant exhibit occasional misrouting of ventral axons towards the dorsal lamina. In extreme cases, ventral axons appear as if they are about to project to ventral lamina but then veer towards the dorsal lamina. Ventral misrouting is seen in the larval optic stalk.

(Sato et al., 2006)

In ovaries from females homozygous for Wnt4^C1, ovarioles exhibit a "branched ovariole" phenotype, in which multiple ovarioles are attached to one egg chamber.

(Gupta and Schupbach, 2003)

Lethality acts in first or second larval instar. 10%-15% of mutants survive to adulthood. Survivors are sterile, though show no external defects. Phenotype of homozygotes is similar to that of hemizygotes.

(Cohen et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Wnt4^C1 has abnormal neuroanatomy phenotype, enhanceable by hep^r75

(Sato et al., 2006)

Enhancer of

Statement

Reference

Wnt4^C1/Wnt4[+] is an enhancer of increased cell number | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Wnt4^C1/Wnt4[+] is an enhancer of abnormal neuroanatomy phenotype of hep^r75

(Sato et al., 2006)

NOT Enhancer of

Statement

Reference

Wnt4^C1/Wnt4[+] is a non-enhancer of partially lethal phenotype of Nab2^ex3

(Lee et al., 2020)

Wnt4^C1/Wnt4[+] is a non-enhancer of some die during P-stage phenotype of Nab2^ex3

(Lee et al., 2020)

Suppressor of

Statement

Reference

Wnt4^C1/Wnt4[+] is a suppressor | partially of decreased size | adult stage phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Lee et al., 2020)

Wnt4^C1/Wnt4[+] is a suppressor | partially of visible | adult stage phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Lee et al., 2020)

NOT Suppressor of

Statement

Reference

Wnt4^C1/Wnt4[+] is a non-suppressor of partially lethal phenotype of Nab2^ex3

(Lee et al., 2020)

Wnt4^C1/Wnt4[+] is a non-suppressor of some die during P-stage phenotype of Nab2^ex3

(Lee et al., 2020)

Phenotype Manifest In

Enhanced by

Statement

Reference

Wnt4^C1 has neuron phenotype, enhanceable by hep^r75

(Sato et al., 2006)

Wnt4^C1 has lamina phenotype, enhanceable by hep^r75

(Sato et al., 2006)

Wnt4^C1 has eye photoreceptor cell phenotype, enhanceable by hep^r75

(Sato et al., 2006)

Wnt4^C1 has embryonic/larval optic stalk phenotype, enhanceable by hep^r75

(Sato et al., 2006)

Enhancer of

Statement

Reference

Wnt4^C1/Wnt4[+] is an enhancer of embryonic/larval hemocyte | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Wnt4^C1/Wnt4[+] is an enhancer of neuron phenotype of hep^r75

(Sato et al., 2006)

Wnt4^C1/Wnt4[+] is an enhancer of lamina phenotype of hep^r75

(Sato et al., 2006)

Wnt4^C1/Wnt4[+] is an enhancer of eye photoreceptor cell phenotype of hep^r75

(Sato et al., 2006)

NOT Enhancer of

Statement

Reference

Wnt4^C1/Wnt2^L is a non-enhancer of lateral transverse muscle cell | embryonic stage 16 phenotype of Wnt5⁴⁰⁰

(Lahaye et al., 2012)

Suppressor of

Statement

Reference

Wnt4^C1/Wnt4[+] is a suppressor | partially of eye phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Lee et al., 2020)

Other

Statement

Reference

Wnt2^L, Wnt4^C1 has lateral transverse muscle cell | embryonic stage 16 phenotype

(Lahaye et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

No lateral transverse muscle defects are observed in Wnt2^L Wnt4^C1 double mutant stage 16 embryos.

Wnt2^L and Wnt4^C1 do not enhance the lateral transverse muscle attachment defects seen in Wnt5⁴⁰⁰ mutant stage 16 embryos.

(Lahaye et al., 2012)

A Wnt4^C1/+ background enhances the hep^r75 R axon misrouting phenotype. Along with ventral-to-dorsal misroutings, these mutants also show dorsal axons projecting to the medial region of the lamina.

A hep^r75 background enhances the Wnt4^C1/+ R axon misrouting phenotype. Along with ventral-to-dorsal misroutings, these mutants also show dorsal axons projecting to the medial region of the lamina.

(Sato et al., 2006)

Xenogenetic Interactions

Statement

Reference

One copy of Wnt4^C1 strongly enhances the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cSa is expressed under the control of Scer\GAL4^Hml.Δ.

(Sinenko et al., 2010)

Complementation and Rescue Data

Fails to complement

Wnt4^EMS23

(Cohen et al., 2002)

Wnt4^X1

(Cohen et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

6651

w¹¹¹⁸; Wnt4^C1/CyO

6907

y¹ w¹¹¹⁸; Wnt4^C1 Wnt2^L/CyO-Df(2R)B80, y⁺

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

DWnt4^C1

(Takagi et al., 2025, Cohen et al., 2002)

DWnt4C1

(Joshi et al., 2007)

Wnt4^C1

(Thyagarajan et al., 2025, Jetti et al., 2023, Mieszczanek et al., 2022, Kahsai and Cook, 2018, Ripp et al., 2018, Mottier-Pavie et al., 2016, Linnemannstöns et al., 2014, Kuroda et al., 2012, Lahaye et al., 2012, Christensen and Cook, 2007.5.8, Christensen et al., 2007.10.29, Christensen and Cook, 2006.12.5, Sato et al., 2006)

Wnt4^c1

(Lee et al., 2020)

Wnt^4C1

(Sinenko et al., 2010)

wnt4^C1

(Nguyen et al., 2022, Peradziryi et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (21)

Report Sections

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