FlyBase Allele Report: Dmel\Wnt4[EMS23]

General Information

Symbol

Dmel\Wnt4^EMS23

Species

D. melanogaster

Name

FlyBase ID

FBal0141320

Feature type

allele

Associated gene

Dmel\Wnt4

Associated Insertion(s)

Carried in Construct

Also Known As

DWnt4^EMS23

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Cohen et al., 2002)

Mutagen

ethyl methanesulfonate

(Cohen et al., 2002)

Progenitor genotype

Cytology

Description

Amino acid replacement: ?343term.

(Cohen et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:7,256,874..7,256,874 [-]

Nucleotide change:

G7256874A

Amino acid change:

W346term | Wnt4-PA; W346term | Wnt4-PB

Reported amino acid change:

?343term

Comment:

Approximate location of nonsense mutation in Wnt4^EMS23. Curator note: The mapped location is 3 aa downstream of the reported location because the codon at position 346 is GAC, which requires two nucleotide changes to create a nonsense mutation and the reported location of Wnt4^C1 from the same paper appears to be 3aa upstream of its location in the reference sequence.

(Cohen et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates acute myeloid leukemia

modeled by Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa

(Sinenko et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration

(Chen et al., 2016)

abnormal cell shape

(Chen et al., 2016)

abnormal neuroanatomy (with Wnt4^C1)

(Sato et al., 2006)

abnormal neuroanatomy (with Wnt4^P23)

(Sato et al., 2006)

abnormal neuroanatomy | larval stage (with Wnt4^C1)

(Mieszczanek et al., 2022)

abnormal neuroanatomy | P-stage (with Wnt4^P23)

(Han et al., 2020)

partially lethal - majority die | recessive

(Cohen et al., 2002)

some die during larval stage | recessive

(Cohen et al., 2002)

sterile | recessive

(Cohen et al., 2002)

Phenotype Manifest In

axon | larval stage (with Wnt4^C1)

(Mieszczanek et al., 2022)

embryonic/larval heart (with Df(2L)BSC226)

(Chen et al., 2016)

embryonic/larval heart | embryonic stage 14

(Chen et al., 2016)

embryonic/larval optic stalk (with Wnt4^C1)

(Sato et al., 2006)

embryonic cardioblast (with Df(2L)BSC226)

(Chen et al., 2016)

embryonic cardioblast | embryonic stage 14

(Chen et al., 2016)

embryonic somatic muscle cell

(Chen et al., 2016)

eye photoreceptor cell (with Wnt4^C1)

(Sato et al., 2006)

eye photoreceptor cell (with Wnt4^P23)

(Sato et al., 2006)

lamina (with Wnt4^C1)

(Sato et al., 2006)

lamina (with Wnt4^P23)

(Sato et al., 2006)

larval brain | larval stage (with Wnt4^C1)

(Mieszczanek et al., 2022)

larval ostial pore

(Chen et al., 2016)

larval ostial pore (with Df(2L)BSC226)

(Chen et al., 2016)

medulla | P-stage (with Wnt4^P23)

(Han et al., 2020)

medulla intrinsic neuron Mi1 | P-stage (with Wnt4^P23)

(Han et al., 2020)

neuron (with Wnt4^C1)

(Sato et al., 2006)

neuron (with Wnt4^P23)

(Sato et al., 2006)

photoreceptor cell R8 | P-stage (with Wnt4^P23)

(Han et al., 2020)

presumptive embryonic salivary gland

(Harris and Beckendorf, 2007)

Detailed Description

Statement

Reference

Homozygous Wnt4^EMS23 and transheterozygous Wnt4^EMS23/Df(2L)BSC226 embryos have cardioblast alignment defects obvious at stage 14, worsening as development progresses and resulting in gaps within the heart tube. In contrast, cardiac progenitor cell specification and pericardial cells are not affected.

In Wnt4^EMS23 homozygous embryos, ostia cell progenitors fail to undergo the cellular constriction typically observed in wild-type hearts. This impairs ostia cell formation and leads to loss of ostia.

(Chen et al., 2016)

One copy of Wnt4^EMS23 has no effect on hemocyte proliferation in third instar larvae.

(Sinenko et al., 2010)

Mutant embryos have salivary gland guidance defects; the entire salivary gland is curved ventromedially, instead of lying parallel to the midline as in wild-type embryos. This defect is seen as early as stage 12.

(Harris and Beckendorf, 2007)

Wnt4^C1/Wnt4^EMS23 mutant exhibit occasional misrouting of ventral axons towards the dorsal lamina. In extreme cases, ventral axons appear as if they are about to project to ventral lamina but then veer towards the dorsal lamina. Ventral misrouting is seen in the larval optic stalk.

(Sato et al., 2006)

Dorsal cluster neuron axon extension is not affected in Wnt4^EMS23/+ flies.

(Srahna et al., 2006)

Lethality acts in first or second larval instar. 10%-15% of mutants survive to adulthood. Survivors are sterile, though show no external defects. Phenotype of homozygotes is similar to that of hemizygotes.

(Cohen et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Wnt4^EMS23 has abnormal neuroanatomy phenotype, enhanceable by hep^r75

(Sato et al., 2006)

Enhancer of

Statement

Reference

Wnt4[+]/Wnt4^EMS23 is an enhancer of increased cell number | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Wnt4[+]/Wnt4^EMS23 is an enhancer of abnormal neuroanatomy phenotype of hep^r75

(Sato et al., 2006)

Suppressor of

Statement

Reference

Wnt4[+]/Wnt4^EMS23 is a suppressor of decreased size | adult stage phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Lee et al., 2020)

Wnt4[+]/Wnt4^EMS23 is a suppressor of visible | adult stage phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Lee et al., 2020)

Phenotype Manifest In

Enhanced by

Statement

Reference

Wnt4^EMS23 has presumptive embryonic salivary gland phenotype, enhanceable by Wnt5^D7

(Harris and Beckendorf, 2007)

Wnt4^EMS23 has neuron phenotype, enhanceable by hep^r75

(Sato et al., 2006)

Wnt4^EMS23 has lamina phenotype, enhanceable by hep^r75

(Sato et al., 2006)

Wnt4^EMS23 has eye photoreceptor cell phenotype, enhanceable by hep^r75

(Sato et al., 2006)

Wnt4^EMS23 has embryonic/larval optic stalk phenotype, enhanceable by hep^r75

(Sato et al., 2006)

Enhancer of

Statement

Reference

Wnt4[+]/Wnt4^EMS23 is an enhancer of embryonic/larval hemocyte | third instar larval stage phenotype of Hsap\RUNX1::Hsap\RUNX1T1^UAS.cSa, Scer\GAL4^Hml.Δ

(Sinenko et al., 2010)

Wnt4^EMS23 is an enhancer of presumptive embryonic salivary gland phenotype of Wnt5^D7

(Harris and Beckendorf, 2007)

Wnt4[+]/Wnt4^EMS23 is an enhancer of eye photoreceptor cell phenotype of hep^r75

(Sato et al., 2006)

Wnt4[+]/Wnt4^EMS23 is an enhancer of neuron phenotype of hep^r75

(Sato et al., 2006)

Wnt4[+]/Wnt4^EMS23 is an enhancer of lamina phenotype of hep^r75

(Sato et al., 2006)

NOT Enhancer of

Statement

Reference

Wnt4^EMS23 is a non-enhancer of interneuron phenotype of Scer\GAL4^eg-Mz360, drl^UAS.cCa

(Yoshikawa et al., 2003)

Suppressor of

Statement

Reference

Wnt4[+]/Wnt4^EMS23 is a suppressor of eye phenotype of Nab2^EP3716, Scer\GAL4^GMR.PU

(Lee et al., 2020)

NOT Suppressor of

Statement

Reference

Wnt4^EMS23 is a non-suppressor of interneuron phenotype of Scer\GAL4^eg-Mz360, drl^UAS.cCa

(Yoshikawa et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Wnt4^EMS23/Wnt4^P23 does not enhance the neuronal targeting defect seen in Df(3R)Tl-X/Df(3R)ro-XB3 embryos, in which the nerve terminals synapsed to muscle M12 are greatly reduced and the nerve terminals synapsed to muscle M13 are expanded compared to controls.

(Inaki et al., 2010)

Wnt5^D7 ; Wnt4^EMS23 double mutants have a higher penetrance of salivary gland curving than is seen in either of the single mutants alone.

(Harris and Beckendorf, 2007)

A hep^r75 bacgkground enhances the Wnt4^EMS23/+ R axon misrouting phenotype.

A Wnt4^EMS23/+ background enhances the hep^r75 R axon misrouting phenotype.

(Sato et al., 2006)

The addition of Wnt4^EMS23 has no effect on the interneuron phenotype seen in drl^Scer\UAS.cCa, Scer\GAL4^eg-Mz360 animals.

(Yoshikawa et al., 2003)

Xenogenetic Interactions

Statement

Reference

One copy of Wnt4^EMS23 strongly enhances the hemocyte overproliferation seen in third instar larvae when Hsap\RUNX1::Hsap\RUNX1T1^Scer\UAS.cSa is expressed under the control of Scer\GAL4^Hml.Δ.

(Sinenko et al., 2010)

Complementation and Rescue Data

Fails to complement

Wnt4^C1

(Cohen et al., 2002)

Wnt4^X1

(Cohen et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

6650

Wnt4^EMS23 bw¹/CyO, P{HB-lacZ}GS1

Kyoto

108974

Wnt4^EMS23 bw¹/CyO, P{HB-lacZ}GS1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

DWnt4^EMS23

(Takagi et al., 2025, Han et al., 2020, Cohen et al., 2002)

DWnt4EMS23

(Joshi et al., 2007)

Wnt4^EMS23

(Thyagarajan et al., 2025, Mieszczanek et al., 2022, Chen et al., 2016, Mottier-Pavie et al., 2016, Kuroda et al., 2012, Inaki et al., 2010, Harris and Beckendorf, 2007, Sato et al., 2006)

Wnt4^EMSS23

(Linnemannstöns et al., 2014)

Wnt4^ems23

(Lee et al., 2020)

wnt4^EMS23

(Nguyen et al., 2022, Peradziryi et al., 2011, Sinenko et al., 2010, Srahna et al., 2006, Yoshikawa et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (19)

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