FlyBase Allele Report: Dmel\Wnt5[400]

Mutant animals show defects in the mushroom body: approximately 30% lack the α lobe, while approximately 50% lack both the α and β lobes. In those animals where α axons are affected, approximately 60% of the α axons show misguidance, while the rest show growth defects. Mushroom body αβ neurons which show guidance defects in both the α and β branches are seen.

(Reynaud et al., 2015)

In 17% of the hemisegments in Wnt5⁴⁰⁰ stage 16 embryos, lateral transverse muscles (LTMs) bypass their normal attachment at the epidermis at muscle 12 and instead extend ventrally beyond muscle 13 and attach at a novel epidermal site located close to muscle fiber 7. Occasionally, either muscle 6 or muscle 7 is absent. This LTM overextension phenotype is seen in 8% of third instar larvae.

The epidermal clusters of tendon cell precursors in stage 11/12 Wnt5⁴⁰⁰ mutant embryos are similar in size and location to wild type clusters.

(Lahaye et al., 2012)

At embryonic stage 17, Wnt5⁴⁰⁰ mutants exhibit broken Fas2 fascicles mainly in the lateral pathway.

The early trajectories of pioneer axons in Wnt5⁴⁰⁰ mutants are similar to that of wild-type embryos.

Loss of Wnt5 in Wnt5⁴⁰⁰ mutants affects the defasciculation of the MP1/dMP2 and pcCC/vMP2 pathways, resulting in a single fascicle, thinning or breaks.

(Ding et al., 2011)

Approximately 67% of antennal lobes are not connected by commissures in homozygous adults. The antennal lobes appear flattened dorsally and elongated ventrally, due to a 'collapse' of the dorsomedial region of the lobe. The olfactory receptor neuron (ORN) axons show a number of targeting defects, including taking meandering paths to their targets, looping back on the ipsilateral glomeruli, projecting aberrantly to dorsal regions of the brain and terminating on ectopic glomeruli. The pattern of projection neuron dendrites in the antennal lobe neuropil is disrupted and many dendritic arbors are displaced ventrally. The misshapen antennal lobe phenotype is apparent by 50 hours after puparium formation.

The antennal lobes are misshapen in animals carrying large homozygous ORN clones. Scer\GAL4^OK72-positive ORNs project to a slightly more dorsal position than that of controls and form distorted and split glomeruli. Their contralateral axons often appear defasciculated and fail to cross the midline (instead looping back on their ipsilateral targets).

(Yao et al., 2007)

The brains of Wnt5⁴⁰⁰ flies show a reduction in the number of dorsal cluster neuron axons that cross the optic chiasm (from 11.7 on average in wild type to 7.9 in Wnt5⁴⁰⁰ mutants).

(Srahna et al., 2006)

Wnt5⁴⁰⁰/Df(1)E128 embryos show CNS phenotypes of similar severity to those seen in Wnt5⁴⁰⁰ homozygous mutants, indicating Wnt5⁴⁰⁰ is a null allele.

(Fradkin et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Wnt5⁴⁰⁰ has abnormal neuroanatomy phenotype, enhanceable by uzip^D43

(Ding et al., 2011)

Wnt5⁴⁰⁰ has abnormal neuroanatomy phenotype, enhanceable by fz[+]/fz¹⁵

(Srahna et al., 2006)

Wnt5⁴⁰⁰ has abnormal neuroanatomy phenotype, enhanceable by fz2[+]/fz2^C1

(Srahna et al., 2006)

Wnt5⁴⁰⁰ has abnormal neuroanatomy phenotype, enhanceable by dsh¹/dsh[+]

(Srahna et al., 2006)

NOT Enhanced by

Statement

Reference

Wnt5⁴⁰⁰ has viable phenotype, non-enhanceable by Ror[+]/Ror⁴

(Ripp et al., 2018)

Suppressed by

Statement

Reference

Wnt5⁴⁰⁰ has abnormal neuroanatomy phenotype, suppressible by CadN^M19

(Ding et al., 2011)

NOT suppressed by

Statement

Reference

Wnt5⁴⁰⁰ has viable phenotype, non-suppressible by Ror[+]/Ror⁴

(Ripp et al., 2018)

Enhancer of

Statement

Reference

Wnt5⁴⁰⁰/Wnt5[+] is an enhancer of abnormal neuroanatomy phenotype of DAAM^Ex1

(Gombos et al., 2015)

Wnt5⁴⁰⁰/Wnt5[+] is an enhancer of abnormal neuroanatomy phenotype of fz¹⁵

(Srahna et al., 2006)

Wnt5⁴⁰⁰/Wnt5[+] is an enhancer of abnormal neuroanatomy phenotype of dsh¹

(Srahna et al., 2006)

NOT Enhancer of

Statement

Reference

Wnt5⁴⁰⁰/Wnt5[+] is a non-enhancer of abnormal neuroanatomy | adult stage | dominant phenotype of tap^Gal4

(Yuan et al., 2016)

Suppressor of

Statement

Reference

Wnt5⁴⁰⁰ is a suppressor of abnormal neuroanatomy | embryonic stage phenotype of Drl-2^UAS.Tag:MYC, Scer\GAL4^eg-Mz360

(Reynaud et al., 2015)

Wnt5⁴⁰⁰ is a suppressor of abnormal neuroanatomy phenotype of CadN^M19

(Ding et al., 2011)

NOT Suppressor of

Statement

Reference

Wnt5⁴⁰⁰ is a non-suppressor of homeotic | adult stage phenotype of Scer\GAL4^ey.PH, wge^UAS.Tag:HA

(Nemoto et al., 2023)

Wnt5⁴⁰⁰ is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^ey.PH, wge^UAS.Tag:HA

(Nemoto et al., 2023)

Other

Statement

Reference

Ror⁴, Wnt5⁴⁰⁰ has lethal phenotype

(Ripp et al., 2018)

Wnt5⁴⁰⁰/Df(1)N19, Df(2L)ED729/Ror⁴ has viable phenotype

(Ripp et al., 2018)

Df(2L)ED729/Ror⁴, Wnt5⁴⁰⁰/Wnt5^Gal4 has viable phenotype

(Ripp et al., 2018)

Phenotype Manifest In

Enhanced by

Statement

Reference

Wnt5⁴⁰⁰ has lateral transverse muscle cell | embryonic stage 16 phenotype, enhanceable by drl^Red2

(Lahaye et al., 2012)

Wnt5⁴⁰⁰ has fascicle phenotype, enhanceable by uzip^D43

(Ding et al., 2011)

Wnt5⁴⁰⁰ has presumptive embryonic/larval central nervous system phenotype, enhanceable by uzip^D43

(Ding et al., 2011)

Wnt5⁴⁰⁰ has lateral longitudinal fascicle phenotype, enhanceable by uzip^D43

(Ding et al., 2011)

Wnt5⁴⁰⁰ has larval MP1 neuron phenotype, enhanceable by uzip^D43

(Ding et al., 2011)

Wnt5⁴⁰⁰ has MP1 tract phenotype, enhanceable by uzip^D43

(Ding et al., 2011)

Wnt5⁴⁰⁰ has vMP2 tract phenotype, enhanceable by uzip^D43

(Ding et al., 2011)

Wnt5⁴⁰⁰ has axon & dorsal cluster neuron phenotype, enhanceable by fz[+]/fz¹⁵

(Srahna et al., 2006)

Wnt5⁴⁰⁰ has axon & dorsal cluster neuron phenotype, enhanceable by fz2[+]/fz2^C1

(Srahna et al., 2006)

Wnt5⁴⁰⁰ has axon & dorsal cluster neuron phenotype, enhanceable by dsh¹/dsh[+]

(Srahna et al., 2006)

NOT Enhanced by

Statement

Reference

Wnt5⁴⁰⁰ has lateral transverse muscle cell | embryonic stage 16 phenotype, non-enhanceable by Wnt4^C1/Wnt2^L

(Lahaye et al., 2012)

Suppressed by

Statement

Reference

Wnt5⁴⁰⁰ has larval MP1 neuron phenotype, suppressible by CadN^M19

(Ding et al., 2011)

Wnt5⁴⁰⁰ has MP1 tract phenotype, suppressible by CadN^M19

(Ding et al., 2011)

Wnt5⁴⁰⁰ has vMP2 tract phenotype, suppressible by CadN^M19

(Ding et al., 2011)

Wnt5⁴⁰⁰ has fascicle phenotype, suppressible by CadN^M19

(Ding et al., 2011)

Wnt5⁴⁰⁰ has presumptive embryonic/larval central nervous system phenotype, suppressible by CadN^M19

(Ding et al., 2011)

Wnt5⁴⁰⁰ has lateral longitudinal fascicle phenotype, suppressible by CadN^M19

(Ding et al., 2011)

NOT suppressed by

Statement

Reference

Wnt5⁴⁰⁰ has adult antennal lobe phenotype, non-suppressible by drl[+]/drl²

(Yao et al., 2007)

Wnt5⁴⁰⁰ has adult antennal lobe phenotype, non-suppressible by drl²/drl²

(Yao et al., 2007)

Enhancer of

Statement

Reference

Wnt5⁴⁰⁰/Wnt5[+] is an enhancer of adult mushroom body alpha-lobe phenotype of DAAM^Ex1

(Gombos et al., 2015)

Wnt5⁴⁰⁰/Wnt5[+] is an enhancer of adult mushroom body beta-lobe phenotype of DAAM^Ex1

(Gombos et al., 2015)

Wnt5⁴⁰⁰/Wnt5[+] is an enhancer of axon & dorsal cluster neuron phenotype of fz¹⁵

(Srahna et al., 2006)

Wnt5⁴⁰⁰/Wnt5[+] is an enhancer of axon & dorsal cluster neuron phenotype of dsh¹

(Srahna et al., 2006)

NOT Enhancer of

Statement

Reference

Wnt5⁴⁰⁰/Wnt5[+] is a non-enhancer of adult mushroom body alpha-lobe | adult stage phenotype of tap^Gal4

(Yuan et al., 2016)

Suppressor of

Statement

Reference

Wnt5⁴⁰⁰ is a suppressor of larval posterior commissure | embryonic stage phenotype of Drl-2^UAS.Tag:MYC, Scer\GAL4^eg-Mz360

(Reynaud et al., 2015)

Wnt5⁴⁰⁰ is a suppressor of larval MP1 neuron phenotype of CadN^M19

(Ding et al., 2011)

Wnt5⁴⁰⁰ is a suppressor of dMP2 neuron phenotype of CadN^M19

(Ding et al., 2011)

Wnt5⁴⁰⁰ is a suppressor of pCC neuron phenotype of CadN^M19

(Ding et al., 2011)

Wnt5⁴⁰⁰ is a suppressor of MP1 tract phenotype of CadN^M19

(Ding et al., 2011)

Wnt5⁴⁰⁰ is a suppressor of vMP2 tract phenotype of CadN^M19

(Ding et al., 2011)

Wnt5⁴⁰⁰/Wnt5[+] is a suppressor of adult antennal lobe phenotype of drl²

(Yao et al., 2007)

NOT Suppressor of

Statement

Reference

Wnt5⁴⁰⁰ is a non-suppressor of eye phenotype of Scer\GAL4^ey.PH, wge^UAS.Tag:HA

(Nemoto et al., 2023)

Wnt5⁴⁰⁰ is a non-suppressor of lateral transverse muscle cell | embryonic stage 16 phenotype of drl^Red2

(Lahaye et al., 2012)

Other

Statement

Reference

Df(2L)Exel6043, Wnt5⁴⁰⁰ has lateral transverse muscle cell | embryonic stage 16 phenotype

(Lahaye et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Wnt5⁴⁰⁰/+ does not significantly enhance frequency of mushroom body beta lobe axon overgrowth defects in tap^Gal4/+ brains.

(Yuan et al., 2016)

One copy of Wnt5⁴⁰⁰ enhances the defects seen in the axonal projection defects seen in the α and β lobes of DAAM^Ex1 mutant mushroom bodies.

(Gombos et al., 2015)

The axon commissure switching phenotype seen in embryos expressing two copies of Drl-2^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^eg-Mz360 is completely suppressed by Wnt5⁴⁰⁰/Y.

(Reynaud et al., 2015)

In 36% of the hemisegments in drl^Red2 Wnt5⁴⁰⁰ double mutant embryos, the lateral transverse muscles (LTMs) bypass their normal attachment at the epidermis at muscle 12 and instead extend ventrally beyond muscle 13 and attach at a novel epidermal site located close to muscle fiber 7. This is similar to the number seen in drl^Red2 single mutants, but significantly more than is seen in Wnt5⁴⁰⁰ mutants.

In 16% of the hemisegments in Wnt5⁴⁰⁰/+ ; Df(2L)Exel6043/+ stage 16 embryos, the lateral transverse muscles (LTMs) bypass their normal attachment at the epidermis at muscle 12 and instead extend ventrally beyond muscle 13 and attach at a novel epidermal site located close to muscle fiber 7. In males that are hemizygous for Wnt5⁴⁰⁰ and heterozygous for Df(2L)Exel6043 27% of hemisegments show attachment defects.

Wnt2^L and Wnt4^C1 do not enhance the lateral transverse muscle attachment defects seen in Wnt5⁴⁰⁰ mutant stage 16 embryos.

(Lahaye et al., 2012)

Wnt5⁴⁰⁰ uzip^D43 double mutants exhibit an enhancement of the broken fascicles found in Wnt5⁴⁰⁰ single mutants.

The early trajectories of pioneer axons in CadN^M19 Wnt5⁴⁰⁰ double mutants are similar to that of wild-type embryos.

Wnt5⁴⁰⁰ uzip^D43 double mutants exhibit an enhancement of the defasciculation of the MP1/dMP2 and pcCC/vMP2 pathways.

(Ding et al., 2011)

drl²/+ has no effect on the antennal lobe phenotype of Wnt5⁴⁰⁰ homozygotes.

Wnt5⁴⁰⁰/Y drl²/drl² double mutants have antennal lobes with the characteristic shape seen in Wnt5⁴⁰⁰/Y single mutants.

Wnt5⁴⁰⁰/+ suppresses the antennal lobe phenotypes of drl² mutants (both the dorsomedial antennal lobe protrusions and the glomerular defects are suppressed).

(Yao et al., 2007)

Wnt5⁴⁰⁰/+, dsh¹/+ double mutants show a more severe dorsal cluster neuron axon phenotype than either single mutant.

Wnt5⁴⁰⁰/+; fz¹⁵/+ brains show a synergistic reduction in the number of dorsal cluster neuron axons that reach the medulla.

Wnt5⁴⁰⁰/+; fz2^C1/+ brains show a small but significant decrease in the number of dorsal cluster neuron axons that reach the medulla compared to Wnt5⁴⁰⁰/+ brains.

(Srahna et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Wnt5⁴⁰⁰ is rescued by Scer\GAL4^how-24B/Wnt5^UAS.cFa

(Lahaye et al., 2012)

Wnt5⁴⁰⁰ is rescued by Scer\GAL4^Mef2.PR/Wnt5^UAS.cFa

(Lahaye et al., 2012)

Wnt5⁴⁰⁰ is rescued by Scer\GAL4^sr-md710/Wnt5^UAS.cFa

(Lahaye et al., 2012)

Wnt5⁴⁰⁰ is rescued by Wnt5^UAS.cFa/Scer\GAL4^elav.PLu

(Fradkin et al., 2004)

Partially rescued by

Wnt5⁴⁰⁰ is partially rescued by Scer\GAL4^SG18.1/Wnt5^UAS.cFa

(Yao et al., 2007)

Wnt5⁴⁰⁰ is partially rescued by Wnt5^UAS.cFa/Scer\GAL4^OK72

(Yao et al., 2007)

Not rescued by

Wnt5⁴⁰⁰ is not rescued by Scer\GAL4^tey-5053A/Wnt5^UAS.cFa

(Lahaye et al., 2012)

Wnt5⁴⁰⁰ is not rescued by Wnt5^UAS.cFa/Scer\GAL4^GH146

(Yao et al., 2007)

Wnt5⁴⁰⁰ is not rescued by Scer\GAL4^Ntan1-Mz317/Wnt5^UAS.cFa

(Yao et al., 2007)

Wnt5⁴⁰⁰ is not rescued by Scer\GAL4^repo/Wnt5^UAS.cFa

(Fradkin et al., 2004)

Wnt5⁴⁰⁰ is not rescued by Wnt5^UAS.cFa/Scer\GAL4^sim.PS

(Fradkin et al., 2004)

Comments

Expression of Wnt5^Scer\UAS.cFa under the control of any of Scer\GAL4^how-24B, Scer\GAL4^Mef2.PR or Scer\GAL4^sr-md710 rescues the lateral transverse muscle attachment defects seen in Wnt5⁴⁰⁰ mutant stage 16 embryos. Expressing Wnt5^Scer\UAS.cFa under the control of Scer\GAL4^tey-5053A is unable to rescue the phenotype.

(Lahaye et al., 2012)

Expression of Wnt5^Scer\UAS.cFa under the control of either Scer\GAL4^GH146 or Scer\GAL4^Mz317 does not rescue the antennal lobe defects seen in Wnt5⁴⁰⁰ flies.

Expression of Wnt5^Scer\UAS.cFa under the control of either Scer\GAL4^SG18.1 or Scer\GAL4^OK72 partially rescues the antennal lobe defects seen in Wnt5⁴⁰⁰ flies: the abnormal shape is rescued, the abnormal position of the Or47b glomerulus relative to the dorsal edge of the lobe is partially rescued and a commissure connects the antennal lobes in 55% or 77% of cases respectively.

(Yao et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

64300

w¹¹¹⁸ Wnt5⁴⁰⁰ P{neoFRT}19A

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

DWnt5⁴⁰⁰

(Takagi et al., 2025)

Wnt5⁴⁰⁰

(Hing et al., 2020, Ripp et al., 2018, Yuan et al., 2016, Gombos et al., 2015, Reynaud et al., 2015, Linnemannstöns et al., 2014, Lahaye et al., 2012, Ding et al., 2011, Shimizu et al., 2011)

wnt5⁴⁰⁰

(Nemoto et al., 2023, Zhu et al., 2019, Wu et al., 2014, Yao et al., 2007, Srahna et al., 2006, Fradkin et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (16)

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