FlyBase Allele Report: Dmel\Wnt5[UAS.cFa]

General Information

Symbol

Dmel\Wnt5^UAS.cFa

Species

D. melanogaster

Name

FlyBase ID

FBal0159535

Feature type

allele

Associated gene

Dmel\Wnt5

Associated Insertion(s)

Carried in Construct

P{UAS-Wnt5.F}

Also Known As

UAS-Wnt5

Key Links

Transgenic product class

wild_type

(Fradkin et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Fradkin et al., 2004)

Mutagen

in vitro construct

(Fradkin et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-Wnt5.F}

Cytology

Description

UAS sequences drive expression of Wnt5.

(Fradkin et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Wnt5

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^c739

(Reynaud et al., 2015)

abnormal neuroanatomy, with Scer\GAL4^ey-OK107

(Gombos et al., 2015)

abnormal neuroanatomy, with Scer\GAL4^sim.PS

(Ding et al., 2011)

abnormal neuroanatomy | adult stage, with Scer\GAL4^GH146

(Wu et al., 2014)

abnormal neuroanatomy | adult stage | somatic clone, with Scer\GAL4^Mz19

(Wu et al., 2014)

abnormal neuroanatomy | adult stage | somatic clone, with Scer\GAL4^unspecified

(Wu et al., 2014)

Phenotype Manifest In

adult antennal lobe, with Scer\GAL4^GH146

(Wu et al., 2014)

adult antennal lobe projection neuron adPN | somatic clone, with Scer\GAL4^unspecified

(Wu et al., 2014)

adult antennal lobe projection neuron DA1 lPN, with Scer\GAL4^Mz19

(Wu et al., 2014)

adult antennal lobe projection neuron DC3 adPN, with Scer\GAL4^Mz19

(Wu et al., 2014)

adult antennal lobe projection neuron lPN | somatic clone, with Scer\GAL4^unspecified

(Wu et al., 2014)

adult antennal lobe projection neuron VA1d adPN, with Scer\GAL4^Mz19

(Wu et al., 2014)

adult mushroom body alpha-lobe, with Scer\GAL4^c739

(Reynaud et al., 2015)

adult mushroom body beta-lobe, with Scer\GAL4^ey-OK107

(Gombos et al., 2015)

dendrite | adult stage, with Scer\GAL4^Mz19

(Wu et al., 2014)

larval anterior commissure, with Scer\GAL4^sim.PS

(Ding et al., 2011, Fradkin et al., 2004)

mushroom body medial lobe, with Scer\GAL4^ey-OK107

(Gombos et al., 2015)

presumptive embryonic salivary gland, with Scer\GAL4^bap.3

(Harris and Beckendorf, 2007)

Detailed Description

Statement

Reference

When Wnt5^Scer\UAS.cFa is expressed in the mushroom body under the control of Scer\GAL4^ey-OK107 the β lobe axons fail to terminate properly at the CNS midline and display medial lobe fusion phenotype.

(Gombos et al., 2015)

Expression of two copies of Wnt5^Scer\UAS.cFa under the control of Scer\GAL4^c739 results in a low but significant fraction of the mushroom bodies showing misguidance of α axon misguidance.

(Reynaud et al., 2015)

Expression of Wnt5^Scer\UAS.cFa in the vPN or DL1 projection neurons (using the MARCM system) does not alter their dendritic pattern.

Expression of Wnt5^Scer\UAS.cFa in the adPN and lPN projection neurons (using the MARCM system) significantly disrupts their dendritic innervation patterns.

Expression of Wnt5^Scer\UAS.cFa under the control of Scer\GAL4^Mz19 results in strong mistargeting of the dendrites in the DA1, VA1d and DC3 projection neurons.

Expression of Wnt5^Scer\UAS.cFa under the control of Scer\GAL4^GH146 results in a severe distortion of the antennal lobe.

(Wu et al., 2014)

Embryos with two copies of Scer\GAL4^sim.PS and Wnt5^Scer\UAS.cFa exhibit thinner or complete loss of anterior commissures.

(Ding et al., 2011)

Expression of Wnt5^Scer\UAS.cFa under the control of Scer\GAL4^bap.3 in the visceral mesoderm is sufficient to redirect the salivary glands ventrally, away from the visceral mesoderm. The ventral curving affects only the tip of the salivary gland.

(Harris and Beckendorf, 2007)

Expression of Wnt5^Scer\UAS.cFa under the control of Scer\GAL4^SG18.1 has no effect on structure of the antennal lobe.

(Yao et al., 2007)

When Wnt5^Scer\UAS.cFa is driven by Scer\GAL4^sim.PS, the posterior commissure appears normal, the anterior commissure sometimes fails to form. The penetrance of this phenotype is dose dependant.

(Fradkin et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^c739, Wnt5^UAS.cFa has abnormal neuroanatomy phenotype, enhanceable by drl[+]/drl²

(Reynaud et al., 2015)

Scer\GAL4^c739, Wnt5^UAS.cFa has abnormal neuroanatomy phenotype, enhanceable by Drl-2^E124/Drl-2[+]

(Reynaud et al., 2015)

Scer\GAL4^c739, Wnt5^UAS.cFa has abnormal neuroanatomy phenotype, enhanceable by drl[+]/drl²/Drl-2^E124/Drl-2[+]

(Reynaud et al., 2015)

NOT Enhanced by

Statement

Reference

Scer\GAL4^sim.PS, Wnt5^UAS.cFa has abnormal neuroanatomy phenotype, non-enhanceable by uzip^D43

(Ding et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^ey-OK107, Wnt5^UAS.cFa has abnormal neuroanatomy phenotype, suppressible by DAAM^Ex1

(Gombos et al., 2015)

Scer\GAL4^ey-OK107, Wnt5^UAS.cFa has abnormal neuroanatomy phenotype, suppressible | partially by dsh¹

(Gombos et al., 2015)

Scer\GAL4^ey-OK107, Wnt5^UAS.cFa has abnormal neuroanatomy phenotype, suppressible | partially by Rac1^J11

(Gombos et al., 2015)

Scer\GAL4^GH146, Wnt5^UAS.cFa has abnormal neuroanatomy | adult stage phenotype, suppressible by Scer\GAL4^GH146/drl^UAS.cYa

(Wu et al., 2014)

NOT suppressed by

Statement

Reference

Scer\GAL4^GH146, Wnt5^UAS.cFa has abnormal neuroanatomy | adult stage phenotype, non-suppressible by drl^{Δintra.UAS.Tag:MYC}/Scer\GAL4^GH146

(Wu et al., 2014)

Scer\GAL4^sim.PS, Wnt5^UAS.cFa has abnormal neuroanatomy phenotype, non-suppressible by uzip^D43

(Ding et al., 2011)

Enhancer of

Statement

Reference

Scer\GAL4^SG18.1/Wnt5^UAS.cFa is an enhancer of abnormal neuroanatomy | adult stage phenotype of drl²

(Yao et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^c739, Wnt5^UAS.cFa has adult mushroom body alpha-lobe phenotype, enhanceable by drl[+]/drl²

(Reynaud et al., 2015)

Scer\GAL4^c739, Wnt5^UAS.cFa has adult mushroom body alpha-lobe phenotype, enhanceable by Drl-2^E124/Drl-2[+]

(Reynaud et al., 2015)

Scer\GAL4^c739, Wnt5^UAS.cFa has adult mushroom body alpha-lobe phenotype, enhanceable by drl[+]/drl²/Drl-2^E124/Drl-2[+]

(Reynaud et al., 2015)

NOT Enhanced by

Statement

Reference

Scer\GAL4^sim.PS, Wnt5^UAS.cFa has larval anterior commissure phenotype, non-enhanceable by uzip^D43

(Ding et al., 2011)

Suppressed by

Statement

Reference

Scer\GAL4^ey-OK107, Wnt5^UAS.cFa has adult mushroom body beta-lobe phenotype, suppressible by DAAM^Ex1

(Gombos et al., 2015)

Scer\GAL4^ey-OK107, Wnt5^UAS.cFa has mushroom body medial lobe phenotype, suppressible by DAAM^Ex1

(Gombos et al., 2015)

Scer\GAL4^ey-OK107, Wnt5^UAS.cFa has mushroom body medial lobe phenotype, suppressible | partially by dsh¹

(Gombos et al., 2015)

Scer\GAL4^ey-OK107, Wnt5^UAS.cFa has mushroom body medial lobe phenotype, suppressible | partially by Rac1^J11

(Gombos et al., 2015)

Scer\GAL4^GH146, Wnt5^UAS.cFa has adult antennal lobe phenotype, suppressible by Scer\GAL4^GH146/drl^UAS.cYa

(Wu et al., 2014)

Scer\GAL4^sim.PS, Wnt5^UAS.cFa has larval anterior commissure phenotype, suppressible by por^PB16

(Fradkin et al., 2004)

NOT suppressed by

Statement

Reference

Scer\GAL4^GH146, Wnt5^UAS.cFa has adult antennal lobe phenotype, non-suppressible by drl^{Δintra.UAS.Tag:MYC}/Scer\GAL4^GH146

(Wu et al., 2014)

Scer\GAL4^sim.PS, Wnt5^UAS.cFa has larval anterior commissure phenotype, non-suppressible by uzip^D43

(Ding et al., 2011)

Enhancer of

Statement

Reference

Scer\GAL4^SG18.1/Wnt5^UAS.cFa is an enhancer of adult antennal lobe phenotype of drl²

(Yao et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Hemizygous DAAM^Ex1 suppresses the β lobe axon overextension and medial lobe fusion phenotype seen when Wnt5^Scer\UAS.cFa is expressed in male flies under the control of Scer\GAL4^ey-OK107.

Hemizygous dsh¹ partially suppresses the medial lobe fusion phenotype seen when Wnt5^Scer\UAS.cFa is expressed in male flies under the control of Scer\GAL4^ey-OK107.

Hemizygous Rac1^J11 partially suppresses the medial lobe fusion phenotype seen when Wnt5^Scer\UAS.cFa is expressed in male flies under the control of Scer\GAL4^ey-OK107.

(Gombos et al., 2015)

The severity of the mushroom body α axon misguidance phenotype seen in flies expressing two copies of Wnt5^Scer\UAS.cFa under the control of Scer\GAL4^c739 is significantly enhanced (to over 10%) if they are also carrying either drl²/+ or Drl-2^E124/+. Flies simultaneously carrying both drl²/+ and Drl-2^E124/+ and expressing two copies of Wnt5^Scer\UAS.cFa under the control of Scer\GAL4^c739 show misguidance of α axons in over 40% of cases.

(Reynaud et al., 2015)

Co-expression of drl^Scer\UAS.cYa, but not of drl^{Δintra.Scer\UAS.T:Hsap\MYC}, suppresses the distortion of the antennal lobe caused by expression of Wnt5^Scer\UAS.cFa under the control of Scer\GAL4^GH146.

(Wu et al., 2014)

A uzip^D43 background does not affect the anterior commissure phenotype found in embryos expressing Wnt5^Scer\UAS.cFa under the control of Scer\GAL4^sim.PS.

(Ding et al., 2011)

Expression of Wnt5^Scer\UAS.cFa under the control of Scer\GAL4^SG18.1 in a drl² background results in large glomeruli at the midline of the antennal lobe in 83% of mutant animals.

(Yao et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Wnt5-Gal4/Wnt5^UAS.cFa rescues Wnt5^Gal4

(Wu et al., 2014)

Scer\GAL4^how-24B/Wnt5^UAS.cFa rescues Wnt5⁴⁰⁰

(Lahaye et al., 2012)

Scer\GAL4^Mef2.PR/Wnt5^UAS.cFa rescues Wnt5⁴⁰⁰

(Lahaye et al., 2012)

Scer\GAL4^sr-md710/Wnt5^UAS.cFa rescues Wnt5⁴⁰⁰

(Lahaye et al., 2012)

Wnt5^UAS.cFa/Scer\GAL4^elav.PLu rescues Wnt5⁴⁰⁰

(Fradkin et al., 2004)

Partially rescues

Scer\GAL4^SG18.1/Wnt5^UAS.cFa partially rescues Wnt5⁴⁰⁰

(Yao et al., 2007)

Wnt5^UAS.cFa/Scer\GAL4^OK72 partially rescues Wnt5⁴⁰⁰

(Yao et al., 2007)

Fails to rescue

Scer\GAL4^peb-GAL4/Wnt5^UAS.cFa fails to rescue Wnt5^unspecified

(Wu et al., 2014)

Scer\GAL4^tey-5053A/Wnt5^UAS.cFa fails to rescue Wnt5⁴⁰⁰

(Lahaye et al., 2012)

Wnt5^UAS.cFa/Scer\GAL4^GH146 fails to rescue Wnt5⁴⁰⁰

(Yao et al., 2007)

Scer\GAL4^Ntan1-Mz317/Wnt5^UAS.cFa fails to rescue Wnt5⁴⁰⁰

(Yao et al., 2007)

Scer\GAL4^repo/Wnt5^UAS.cFa fails to rescue Wnt5⁴⁰⁰

(Fradkin et al., 2004)

Wnt5^UAS.cFa/Scer\GAL4^sim.PS fails to rescue Wnt5⁴⁰⁰

(Fradkin et al., 2004)

Comments

Expression of Wnt5^Scer\UAS.cFa under the control of Scer\GAL4^Wnt5-Gal4 strongly rescues antennal lobe development in Wnt5^Gal4 animals.

Expression of Wnt5^Scer\UAS.cFa under the control of Scer\GAL4^peb-GAL4 fails to rescue antennal lobe development in Wnt5^unspecified animals.

(Wu et al., 2014)

Expression of Wnt5^Scer\UAS.cFa under the control of any of Scer\GAL4^how-24B, Scer\GAL4^Mef2.PR or Scer\GAL4^sr-md710 rescues the lateral transverse muscle attachment defects seen in Wnt5⁴⁰⁰ mutant stage 16 embryos. Expressing Wnt5^Scer\UAS.cFa under the control of Scer\GAL4^tey-5053A is unable to rescue the phenotype.

(Lahaye et al., 2012)

Expression of Wnt5^Scer\UAS.cFa under the control of either Scer\GAL4^GH146 or Scer\GAL4^Mz317 does not rescue the antennal lobe defects seen in Wnt5⁴⁰⁰ flies.

Expression of Wnt5^Scer\UAS.cFa under the control of either Scer\GAL4^SG18.1 or Scer\GAL4^OK72 partially rescues the antennal lobe defects seen in Wnt5⁴⁰⁰ flies: the abnormal shape is rescued, the abnormal position of the Or47b glomerulus relative to the dorsal edge of the lobe is partially rescued and a commissure connects the antennal lobes in 55% or 77% of cases respectively.

(Yao et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

64298

w¹¹¹⁸; P{UAS-Wnt5.F}201

64299

w¹¹¹⁸; P{UAS-Wnt5.F}11C

Notes on Origin

Discoverer