FlyBase Allele Report: Dmel\uzip[D43]

General Information

Symbol

Dmel\uzip^D43

Species

D. melanogaster

Name

FlyBase ID

FBal0249059

Feature type

allele

Associated gene

Dmel\uzip

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele

(Ding et al., 2011)

Mutagen

P-element activity

(Ding et al., 2011)

Progenitor genotype

Cytology

Description

Excision of uzip^f01534 and uzip^f02444 removes the entire uzip coding sequence.

(Ding et al., 2011)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2R:25,020,314..25,029,554 [+]

Comment:

Scer\FRT-mediated recombination between the two progenitor insertions has resulted in the deletion of the genomic sequence between them.

(Ding et al., 2011)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

uzip^D43 mutant embryos exhibit wild-type axonal fascicles.

uzip^D43 mutants do not exhibit defects in Sema-2b-expressing axons.

(Ding et al., 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

uzip^D43/uzip²³ is an enhancer of abnormal neuroanatomy phenotype of CadN^M19

(Ding et al., 2011)

uzip^D43 is an enhancer of abnormal neuroanatomy phenotype of CadN^M19

(Ding et al., 2011)

uzip^D43 is an enhancer of abnormal neuroanatomy phenotype of Wnt5⁴⁰⁰

(Ding et al., 2011)

uzip^D43/uzip[+] is an enhancer of abnormal neuroanatomy phenotype of CadN^M19

(Ding et al., 2011)

NOT Enhancer of

Statement

Reference

uzip^D43 is a non-enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^sim.PS, Wnt5^UAS.cFa

(Ding et al., 2011)

NOT Suppressor of

Statement

Reference

uzip^D43 is a non-suppressor of abnormal neuroanatomy phenotype of Scer\GAL4^sim.PS, Wnt5^UAS.cFa

(Ding et al., 2011)

Phenotype Manifest In

Suppressed by

Statement

Reference

CadN^M19, uzip^D43 has MP1 tract phenotype, suppressible by Scer\GAL4^elav-C155/uzip^UAS.CFP

(Ding et al., 2011)

CadN^M19, uzip^D43 has vMP2 tract phenotype, suppressible by Scer\GAL4^elav-C155/uzip^UAS.CFP

(Ding et al., 2011)

CadN^M19, uzip^D43 has larval MP1 neuron phenotype, suppressible by Scer\GAL4^repo/uzip^UAS.CFP

(Ding et al., 2011)

CadN^M19, uzip^D43 has dMP2 neuron phenotype, suppressible by Scer\GAL4^repo/uzip^UAS.CFP

(Ding et al., 2011)

CadN^M19, uzip^D43 has pCC neuron phenotype, suppressible by Scer\GAL4^repo/uzip^UAS.CFP

(Ding et al., 2011)

CadN^M19, uzip^D43 has MP1 tract phenotype, suppressible by Scer\GAL4^repo/uzip^UAS.CFP

(Ding et al., 2011)

CadN^M19, uzip^D43 has vMP2 tract phenotype, suppressible by Scer\GAL4^repo/uzip^UAS.CFP

(Ding et al., 2011)

CadN^M19, uzip^D43 has larval MP1 neuron phenotype, suppressible by Scer\GAL4^elav-C155/uzip^UAS.CFP

(Ding et al., 2011)

CadN^M19, uzip^D43 has dMP2 neuron phenotype, suppressible by Scer\GAL4^elav-C155/uzip^UAS.CFP

(Ding et al., 2011)

CadN^M19, uzip^D43 has pCC neuron phenotype, suppressible by Scer\GAL4^elav-C155/uzip^UAS.CFP

(Ding et al., 2011)

Enhancer of

Statement

Reference

uzip^D43/uzip[+] is an enhancer of larval anterior commissure phenotype of CadN^M19

(Ding et al., 2011)

uzip^D43/uzip[+] is an enhancer of presumptive embryonic/larval central nervous system phenotype of CadN^M19

(Ding et al., 2011)

uzip^D43/uzip²³ is an enhancer of MP1 tract phenotype of CadN^M19

(Ding et al., 2011)

uzip^D43 is an enhancer of larval anterior commissure phenotype of CadN^M19

(Ding et al., 2011)

uzip^D43 is an enhancer of presumptive embryonic/larval central nervous system phenotype of CadN^M19

(Ding et al., 2011)

uzip^D43 is an enhancer of fascicle phenotype of Wnt5⁴⁰⁰

(Ding et al., 2011)

uzip^D43 is an enhancer of presumptive embryonic/larval central nervous system phenotype of Wnt5⁴⁰⁰

(Ding et al., 2011)

uzip^D43 is an enhancer of lateral longitudinal fascicle phenotype of Wnt5⁴⁰⁰

(Ding et al., 2011)

uzip^D43 is an enhancer of larval MP1 neuron phenotype of Wnt5⁴⁰⁰

(Ding et al., 2011)

uzip^D43 is an enhancer of MP1 tract phenotype of Wnt5⁴⁰⁰

(Ding et al., 2011)

uzip^D43/uzip[+] is an enhancer of larval MP1 neuron phenotype of CadN^M19

(Ding et al., 2011)

uzip^D43 is an enhancer of vMP2 tract phenotype of Wnt5⁴⁰⁰

(Ding et al., 2011)

uzip^D43/uzip[+] is an enhancer of dMP2 neuron phenotype of CadN^M19

(Ding et al., 2011)

uzip^D43/uzip[+] is an enhancer of MP1 tract phenotype of CadN^M19

(Ding et al., 2011)

uzip^D43/uzip²³ is an enhancer of larval anterior commissure phenotype of CadN^M19

(Ding et al., 2011)

uzip^D43/uzip²³ is an enhancer of presumptive embryonic/larval central nervous system phenotype of CadN^M19

(Ding et al., 2011)

uzip^D43/uzip²³ is an enhancer of larval MP1 neuron phenotype of CadN^M19

(Ding et al., 2011)

uzip^D43/uzip²³ is an enhancer of dMP2 neuron phenotype of CadN^M19

(Ding et al., 2011)

NOT Enhancer of

Statement

Reference

uzip^D43 is a non-enhancer of larval anterior commissure phenotype of Scer\GAL4^sim.PS, Wnt5^UAS.cFa

(Ding et al., 2011)

NOT Suppressor of

Statement

Reference

uzip^D43 is a non-suppressor of larval anterior commissure phenotype of Scer\GAL4^sim.PS, Wnt5^UAS.cFa

(Ding et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

A uzip^D43 heterozygous background increases the axonal defects found in CadN^M19 mutants.

A uzip^D43/uzip²³ background increases the axonal defects found in CadN^M19 mutants.

CadN^M19 uzip^D43 double mutants exhibit extensive axonal defects, including broken fascicles in the three longitudinal pathways.

CadN^M19 defects in Sema-2b-expressing neurons are enhanced by heterozygosity and homozygosity of uzip^D43. In the CadN^M19 uzip^D43 double mutant, Sema-2b axons can successfully reach the contralateral side and combine mild defasciculation in commissures. After reaching the contralateral side, some axons stall and some axons turn posteriorly but not anteriorly.

CadN^M19 uzip^D43 double mutants exhibit more defects in MP1/dMP2 and pCC/vMP2 pathways than CadN^M19 single mutants. They do not form normally where defasciculation is supposed to occur, but become fuzzy, thinning or broken, indicating that uzip and CadN cooperatively affect the axonal growth of pioneer axons.

Defects in MP1/dMP2 and pCC/vMP2 pathways mutant for CadN^M19 uzip^D43 can be partially rescued by expression of uzip^{Scer\UAS.T:Avic\GFP-CFP} in glia (under the control of Scer\GAL4^repo) and in neurons (under the control of Scer\GAL4^elav-C155).

Wnt5⁴⁰⁰ uzip^D43 double mutants exhibit an enhancement of the broken fascicles found in Wnt5⁴⁰⁰ single mutants.

Wnt5⁴⁰⁰ uzip^D43 double mutants exhibit an enhancement of the defasciculation of the MP1/dMP2 and pcCC/vMP2 pathways.

(Ding et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

uzip^D43

(Ding et al., 2011)

Name Synonyms

Secondary FlyBase IDs

References (1)

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