FlyBase Allele Report: Dmel\par-1[k05603]

General Information

Symbol

Dmel\par-1^k05603

Species

D. melanogaster

Name

FlyBase ID

FBal0117402

Feature type

allele

Associated gene

Dmel\par-1

Associated Insertion(s)

P{lacW}mei-W68^k05603

Carried in Construct

Also Known As

par-1⁵⁷⁴, l(2)k05603

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Shulman and McKim, 2000.6.20)

Mutagen

P-element activity

(Shulman and McKim, 2000.6.20, Shulman et al., 2000, Tomancak et al., 2000)

Progenitor genotype

Associated Insertion(s)

P{lacW}mei-W68^k05603

Cytology

Description

P{lacW} insertion in the 5' UTR of the par-1 N1 transcription unit. The insertion affects the function of both the par-1 and mei-W68 genes.

(Shulman and McKim, 2000.6.20)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:19,456,950..19,456,957 [+]

(Shulman and McKim, 2000.6.20, Shulman et al., 2000, Tomancak et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female fertile (with par-1^k06323)

(Tomancak et al., 2000)

viable

(Shulman and McKim, 2000.6.20)

Phenotype Manifest In

embryo | posterior (with par-1^k06323)

(Tomancak et al., 2000)

embryonic abdominal segment (with par-1^k06323)

(Tomancak et al., 2000)

germline cell (with par-1^k06323)

(Riechmann et al., 2002)

male germline stem cell (with par-1^k06323)

(Yuan et al., 2012)

primordial germ cell (with par-1^k06323)

(Riechmann et al., 2002)

Detailed Description

Statement

Reference

Germline stem cells (GSCs) in newly eclosed par-1^k06323/par-1^k05603 males show a high frequency of centrosome misorientation and frequently undergo mitosis with misoriented spindles (mitosis with misoriented spindles is not seen in wild-type male GSCs).

(Yuan et al., 2012)

par-1^k05603/+ mutants exhibit ethanol-induced hyperactivity comparable to wild type.

(King et al., 2011)

Embryos derived from par-1^k06323/par-1^k05603 females have an average of 19 pole cells/embryo (compared to 33 in the wild-type), and 12% of the embryos lack a germline.

(Riechmann et al., 2002)

20% of embryos derived from par-1^k05603/par-1^k06323 females die, showing defects which range from disturbances in posterior patterning to complete absence of abdominal segments.

(Tomancak et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

par-1^k05603/par-1[+] is a suppressor of abnormal neuroanatomy | adult stage phenotype of Tao^EP1455

(King et al., 2011)

par-1^k05603/par-1[+] is a suppressor of abnormal chemosensory behavior | adult stage phenotype of Tao^EP1455

(King et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

par-1^k05603/par-1[+] is a suppressor of adult mushroom body alpha-lobe phenotype of Tao^EP1455

(King et al., 2011)

par-1^k05603/par-1[+] is a suppressor of adult mushroom body beta-lobe phenotype of Tao^EP1455

(King et al., 2011)

par-1^k05603/par-1[+] is a suppressor of adult mushroom body gamma-lobe phenotype of Tao^EP1455

(King et al., 2011)

Other

Statement

Reference

osk¹, par-1^k05603/par-1^k06323 has primordial germ cell phenotype

(Riechmann et al., 2002)

osk¹, par-1^k05603/par-1^k06323 has germline cell phenotype

(Riechmann et al., 2002)

osk[+]/osk¹, par-1^k05603/par-1^k06323 has primordial germ cell phenotype

(Riechmann et al., 2002)

osk[+]/osk¹, par-1^k05603/par-1^k06323 has germline cell phenotype

(Riechmann et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of par-1^k05603 partially suppresses the adult mushroom body morphology defects seen in Tao^EP1455. Approximately half of all brain hemispheres have fully formed mushroom body lobes.

One copy of par-1^k05603 suppresses the reduction in ethanol-induced hyperactivity seen in Tao^EP1455 hemizygotes.

(King et al., 2011)

Embryos derived from par-1^k06323/par-1^k05603 ; osk¹/+ females have an average of 3 pole cells/embryo (compared to 33 in the wild-type), and 42% of the embryos lack a germline.

(Riechmann et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

10574