FlyBase Allele Report: Dmel\Iswi[1]

General Information

Symbol

Dmel\Iswi¹

Species

D. melanogaster

Name

FlyBase ID

FBal0117726

Feature type

allele

Associated gene

Dmel\Iswi

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Doyen et al., 2015)

loss of function allele

(Vanolst et al., 2005)

Mutagen

ethyl methanesulfonate

(Deuring et al., 2000)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q953term.

(Deuring et al., 2000)

Nucleotide substitution: C3182T.

(Deuring et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:12,640,110..12,640,110 [+]

Nucleotide change:

C12640110T

Reported nucleotide change:

C3182T

Amino acid change:

Q953term | Iswi-PA; Q953term | Iswi-PB; Q953term | Iswi-PC

Reported amino acid change:

Q953term

(Deuring et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval hemocyte | increased number

(Ragab, 2006)

embryonic/larval salivary gland | larval stage | male (with Iswi²)

(Mengoli et al., 2014)

female germline stem cell | germline clone

(Xi and Xie, 2005)

hemocyte | increased number (with Iswi²)

(Badenhorst et al., 2002)

male germ cell nucleus

(Doyen et al., 2015)

male pronucleus

(Doyen et al., 2015)

polytene chromosome

(Deuring et al., 2000)

polytene chromosome (with Iswi²)

(Siriaco et al., 2009)

polytene chromosome | male (with Iswi²)

(Mengoli et al., 2014, Corona et al., 2007)

polytene chromosome | male limited (with Iswi²)

(Onorati et al., 2011)

spermatozoon

(Doyen et al., 2015)

wing disc (with Iswi²)

(Liu et al., 2008)

Detailed Description

Statement

Reference

The fecundity of Iswi¹/Iswi¹ males is significantly reduced, and mature sperm cells appear to have enlarged nuclei and fail to fully compact their chromatin. Iswi¹/Iswi¹ females lay eggs but are completely sterile. Embryos from Iswi¹/Iswi¹ mothers do not survive beyond nuclear cycle 10, show increased accumulation at the pronuclear apposition stage, and exhibit significant increases in chromatin and mitotic defects, including the male pronucleus remaining abnormally condensed, mis-segregation of chromosomes at anaphase, chromatin bridges in telophase, chromatin exclusion in nuclear cycle 2, asynchronous zygotic divisions, and aneuploidy or haploidy at zygotic divisions.

(Doyen et al., 2015)

Iswi¹/Iswi² transheterozygote males display defects in the morphology of the polytene chromosome X in larval salivary glands. The X chromosome is shorter and more compact compared to wild-type.

(Mengoli et al., 2014)

Polytene chromosomes of Iswi¹/Iswi² male larvae show condensation defects, particularly of the X chromosome.

(Onorati et al., 2011)

The male X polytene chromosome is decondensed in salivary glands of Iswi¹/Iswi² larvae, although the banding pattern is usually retained.

(Siriaco et al., 2009)

Iswi¹/Iswi² animals often survive until the early to mid pupal stages, and have slightly misshapen wing discs at the late third larval instar.

(Liu et al., 2008)

The X polytene chromosome is decondensed in male Iswi¹/Iswi² larvae but not in female Iswi¹/Iswi² larvae.

Mitotic chromosomes prepared from neuroblasts of Iswi¹/Iswi² third instar larvae are indistinguishable from wild type.

(Corona et al., 2007)

Iswi¹ mutants have a high incidence of melanotic tumours and an increased number of larval hemocytes.

(Ragab, 2006)

99 % of clones of Iswi¹ homozygous female germ-line stem cells are lost from their stem cell niche by 17 days after induction, compared to only 35% of wild-type clones. The division rate of Iswi¹ homozygous female germ-line stem cells is around 40% of wild-type, but these mutant cells show no significant increase in apoptosis.

(Xi and Xie, 2005)

The circulating hemocyte cell number in hemolymph isolated from Iswi¹/Iswi² mutants is increased considerably with respect to wild-type animals, having about 12500 per ul^-1, compared to about 4500 in wild-type.

(Badenhorst et al., 2002)

Heterozygotes are viable and phenotypically normal. Hemizygotes die during late larval or early pupal development and show no obvious homeotic transformations or other pattern defects. The structure of the polytene X chromosome of male Iswi¹/Iswi² larvae is much shorter and broader than normal. This alteration in structure of the X chromosome is highly penetrant and is never seen in female Iswi¹/Iswi² larvae. The polytene autosomes are often thinner than normal in both male and female mutant larvae. The structure of mitotic chromosomes of hemizygous larvae appears relatively normal.

(Deuring et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Iswi²/Iswi¹ has lethal | larval stage phenotype, suppressible | partially by Scer\GAL4^Act5C.PI/lncRNA:Hsrω^RNAi.Sym.UAS

(Onorati et al., 2011)

NOT suppressed by

Statement

Reference

Iswi¹ has neoplasia phenotype, non-suppressible by Df(2R)ED3921

(Ragab, 2006)

Suppressor of

Statement

Reference

Iswi[+]/Iswi¹ is a suppressor of visible | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

Iswi[+]/Iswi¹ is a suppressor of visible | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

Other

Statement

Reference

Iswi[+]/Iswi¹, stx² has visible | adult stage phenotype

(Du et al., 2016)

Iswi²/Iswi¹, Scer\GAL4^Act5C.PI, lncRNA:Hsrω^RNAi.Sym.UAS has lethal | pharate adult stage phenotype

(Onorati et al., 2011)

Acf[+]/Acf⁵, Iswi²/Iswi¹ has abnormal developmental rate phenotype

(Liu et al., 2008)

Phenotype Manifest In

Suppressed by

Statement

Reference

Iswi²/Iswi¹ has polytene chromosome | male limited phenotype, suppressible by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^ey.PH

(Onorati et al., 2011)

NOT suppressed by

Statement

Reference

Iswi¹ has embryonic/larval hemocyte | increased number phenotype, non-suppressible by Df(2R)ED3921

(Ragab, 2006)

Enhancer of

Statement

Reference

Iswi[+]/Iswi¹ is an enhancer of dorsal appendage | maternal effect phenotype of EcR^{B1-ΔC655.F645A.UAS}, Scer\GAL4^slbo.2.6

(Badenhorst et al., 2005)

Suppressor of

Statement

Reference

Iswi[+]/Iswi¹ is a suppressor of wing vein | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

Iswi[+]/Iswi¹ is a suppressor of wing vein | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

Other

Statement

Reference

Iswi[+]/Iswi¹, stx² has abdominal tergite phenotype

(Du et al., 2016)

Acf¹, Iswi¹ has abdomen phenotype

(Fyodorov et al., 2004)

Acf¹, Iswi¹ has abdominal tergite 4 phenotype

(Fyodorov et al., 2004)

Acf¹, Iswi¹ has abdominal tergite 5 phenotype

(Fyodorov et al., 2004)

Acf¹, Iswi[+]/Iswi¹ has abdomen phenotype

(Fyodorov et al., 2004)

Acf¹, Iswi[+]/Iswi¹ has abdominal tergite 4 phenotype

(Fyodorov et al., 2004)

Acf¹, Iswi[+]/Iswi¹ has abdominal tergite 5 phenotype

(Fyodorov et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Double heterozygous stuxnet²;Iswi¹ females display tergite boundary defects that are not observed in stuxnet² single heterozygote females.

(Du et al., 2016)

The formation of ectopic wing veins observed in adult flies expressing Marcal1^Scer\UAS.cBa under the control of Scer\GAL4^tub.PU is ameliorated by combination with a single copy of Iswi¹.

(Morimoto et al., 2016)

Expression of Hsrω^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^ey.PH suppresses the X chromosome condensation defects seen in the polytene chromosomes of Iswi¹/Iswi² male larvae.

(Onorati et al., 2011)

Iswi¹/Iswi²; Acf1[5]/+ animals have a significant developmental delay compared to Iswi¹/Iswi² single mutants, surviving to late third larval instar or early pupation.

(Liu et al., 2008)

Flies homozygous for dikar⁵ and heterozygous for Iswi¹ are phenotypically normal - no genetic interaction is seen.

(Keuling et al., 2007)

Df(2R)ED3921 fails to suppress the melanotic tumour and increased larval hemocyte number of Iswi¹ mutants.

(Ragab, 2006)

The frequency of abnormal dorsal appendages in embryos derived from females carrying EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^slbo.2.6 (23%) is increased if the females are also heterozygous for Iswi¹ (76%).

(Badenhorst et al., 2005)

When heterozygous Iswi¹ or Iswi² is combined with homozygous Acf1¹, animals exhibit a homeotic transformation in the abdomen of males. This phenotype is manifested as ectopic reduced pigmentation of the abdominal A5 tergites, consistent with an A4 to A5 transformation. The penetrance (in Acf1¹/Acf1¹, Iswi²/+ animals) is 26%. In addition mutant flies often display an aberrant, asymmetric segmentation of the lower abdomen.

(Fyodorov et al., 2004)

Xenogenetic Interactions

Statement

Reference

The formation of ectopic wing veins observed in adult flies expressing Hsap\SMARCAL1^Scer\UAS.cBa under the control of Scer\GAL4^Bx-MS1096 is ameliorated by combination with a single copy of Iswi¹.

(Morimoto et al., 2016)

Complementation and Rescue Data

Rescued by

Iswi²/Iswi¹ is rescued by Iswi^UAS.Tag:TAP

(Burgio et al., 2008)

Iswi¹ is rescued by Iswi^Tag:HA

(Xi and Xie, 2005)

Iswi¹ is rescued by Iswi^+t12

(Deuring et al., 2000)

Iswi¹ is rescued by Iswi^Tag:HA

(Deuring et al., 2000)

Not rescued by

Iswi¹ is not rescued by Iswi^tBR7

(Deuring et al., 2000)

Iswi¹ is not rescued by Iswi^K159R.Tag:HA

(Deuring et al., 2000)

Iswi¹ is not rescued by Iswi^tBR5

(Deuring et al., 2000)

Comments

Expression of Iswi^{Scer\UAS.T:Zzzz\TAP} under the control of Scer\GAL4^Act5C.PU rescues the lethality of Iswi¹/Iswi² animals.

(Burgio et al., 2008)

Loss of Iswi¹ homozygous clones from their stem cell niche is suppressed by Iswi^T:Ivir\HA1.

(Xi and Xie, 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ISWI¹

(Onorati et al., 2011, Sala et al., 2011, Burgio et al., 2008, Sala et al., 2008)

Iswi¹

(Kwon et al., 2021, Du et al., 2016, Morimoto et al., 2016, Doyen et al., 2015, Mengoli et al., 2014, Nowak et al., 2012, Li et al., 2010, Siriaco et al., 2009, Corona et al., 2007, Keuling et al., 2007)

iswi¹

(Liu et al., 2008, Xi and Xie, 2005)

Name Synonyms

Secondary FlyBase IDs

References (23)

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