FlyBase Allele Report: Dmel\krz[UAS.cRa]

General Information

Symbol

Dmel\krz^UAS.cRa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Roman

FlyBase ID

FBal0118173

Feature type

allele

Associated gene

Dmel\krz

Associated Insertion(s)

Carried in Construct

P{UAS-krz.R}

Also Known As

UAS-Krz

Key Links

Transgenic product class

cDNA

(Roman et al., 2000)

wild_type

(Roman et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Roman et al., 2000)

wild_type

(Roman et al., 2000)

Mutagen

in vitro construct

(Roman et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-krz.R}

Cytology

Description

UASt regulatory sequences drive expression of a full length krz cDNA.

(Roman et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

krz

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior, with Scer\GAL4^hs.PB, krz^S047819

(Liu et al., 2007)

abnormal locomotor behavior, with Scer\GAL4^VP16.Orco, Scer\GAL4^hs.PB, krz^S047819

(Liu et al., 2007)

increased cell number | third instar larval stage, with Scer\GAL4^{E(spl)m6-BFM.PF}

(Aradhya et al., 2015)

Phenotype Manifest In

abdominal lateral adult muscle precursor cell | third instar larval stage, with Scer\GAL4^{E(spl)m6-BFM.PF}

(Aradhya et al., 2015)

Detailed Description

Statement

Reference

Expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^{E(spl)m6-BFM.PF} leads to increased numbers of abdominal lateral adult muscle precursor cells in third instar larvae, as compared to controls.

(Aradhya et al., 2015)

krz^S047819 homozygous adults which have been rescued from lethality by expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^hs.PB show a reduced initial activity phase (during the first minute) in an open field arena compared to control flies. The spontaneous locomotor activity is not significantly different from control flies from the second minute onwards (the assay was carried out for 10 minutes).

There are no significant differences in spontaneous activity between krz^S047819 homozygous adults which have been rescued from lethality by expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^hs.PB and control flies measured in the dark over a period of days.

krz^S047819 homozygous adults which have been rescued from lethality by expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^hs.PB fail to show a robust increase in activity after mechanical stimulation, in contrast to control flies.

(Liu et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^{E(spl)m6-BFM.PF}, krz^UAS.cRa has increased cell number | third instar larval stage phenotype, suppressible by dx^UAS.cMa/Scer\GAL4^{E(spl)m6-BFM.PF}

(Aradhya et al., 2015)

Suppressor of

Statement

Reference

krz^UAS.cRa/Scer\GAL4^{E(spl)m6-BFM.PF} is a suppressor of increased cell number | third instar larval stage phenotype of Scer\GAL4^{E(spl)m6-BFM.PF}, dx^UAS.cMa

(Aradhya et al., 2015)

Other

Statement

Reference

InR^K414P.UAS, Scer\GAL4^{E(spl)m6-BFM.PF}, krz^UAS.cRa has increased cell number | third instar larval stage phenotype

(Aradhya et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^{E(spl)m6-BFM.PF}, krz^UAS.cRa has abdominal lateral adult muscle precursor cell | third instar larval stage phenotype, suppressible by dx^UAS.cMa/Scer\GAL4^{E(spl)m6-BFM.PF}

(Aradhya et al., 2015)

Suppressor of

Statement

Reference

krz^UAS.cRa/Scer\GAL4^{E(spl)m6-BFM.PF} is a suppressor of abdominal lateral adult muscle precursor cell | third instar larval stage phenotype of Scer\GAL4^{E(spl)m6-BFM.PF}, dx^UAS.cMa

(Aradhya et al., 2015)

Other

Statement

Reference

InR^K414P.UAS, Scer\GAL4^{E(spl)m6-BFM.PF}, krz^UAS.cRa has abdominal lateral adult muscle precursor cell | third instar larval stage phenotype

(Aradhya et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of dx^Scer\UAS.cMa and krz^Scer\UAS.cRa under the control of Scer\GAL4^{E(spl)m6-BFM.PF} suppresses the increased number of abdominal lateral adult muscle precursor cells in third instar larvae observed upon expression of either dx^Scer\UAS.cMa or krz^Scer\UAS.cRa alone.

Co-expression of krz^Scer\UAS.cRa and InR^{R418P.Scer\UAS} under the control of Scer\GAL4^{E(spl)m6-BFM.PF} results in increased numbers of abdominal lateral adult muscle precursor cells present in third instar larvae, as compared to controls.

(Aradhya et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

krz^UAS.cRa/Scer\GAL4^hs.PB rescues krz^S047819

(Roman et al., 2000)

Partially rescues

Scer\GAL4^elav-C155/krz^UAS.cRa partially rescues krz^S047819

(Roman et al., 2000)

Fails to rescue

Scer\GAL4^Kr.PM/krz^UAS.cRa fails to rescue krz^S047819

(Roman et al., 2000)

Comments

Expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^hs.PB using heat shock is sufficient to rescue some of the krz^S047819 mutant animals at each larval instar. Rescue of lethality becomes significantly more efficient at older stages. Expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^Kr.PM does not rescue the lethality of krz^S047819 animals. Expression of krz^Scer\UAS.cRa under the control of Scer\GAL4^elav-C155 rescues the lethality of krz^S047819 animals. Approximately 1/3 of the rescued animals fail to expand their wings after eclosion. Melanotic tumours are visible in the abdomens of most of the rescued animals.

(Roman et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

27889

w^*; P{UAS-krz.R}T12

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

krz^Scer\UAS.cRa

krz^UAS.cRa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Roman

(Roman et al., 2000)

Secondary FlyBase IDs

References (5)

Report Sections

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