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General Information
Symbol
Dmel\krzUAS.cRa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Roman
FlyBase ID
FBal0118173
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a full length krz cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of krzScer\UAS.cRa under the control of Scer\GAL4E(spl)m6-BFM.PF leads to increased numbers of abdominal lateral adult muscle precursor cells in third instar larvae, as compared to controls.

krzS047819 homozygous adults which have been rescued from lethality by expression of krzScer\UAS.cRa under the control of Scer\GAL4hs.PB show a reduced initial activity phase (during the first minute) in an open field arena compared to control flies. The spontaneous locomotor activity is not significantly different from control flies from the second minute onwards (the assay was carried out for 10 minutes).

There are no significant differences in spontaneous activity between krzS047819 homozygous adults which have been rescued from lethality by expression of krzScer\UAS.cRa under the control of Scer\GAL4hs.PB and control flies measured in the dark over a period of days.

krzS047819 homozygous adults which have been rescued from lethality by expression of krzScer\UAS.cRa under the control of Scer\GAL4hs.PB fail to show a robust increase in activity after mechanical stimulation, in contrast to control flies.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of dxScer\UAS.cMa and krzScer\UAS.cRa under the control of Scer\GAL4E(spl)m6-BFM.PF suppresses the increased number of abdominal lateral adult muscle precursor cells in third instar larvae observed upon expression of either dxScer\UAS.cMa or krzScer\UAS.cRa alone.

Co-expression of krzScer\UAS.cRa and InRR418P.Scer\UAS under the control of Scer\GAL4E(spl)m6-BFM.PF results in increased numbers of abdominal lateral adult muscle precursor cells present in third instar larvae, as compared to controls.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Fails to rescue
Comments

Expression of krzScer\UAS.cRa under the control of Scer\GAL4hs.PB using heat shock is sufficient to rescue some of the krzS047819 mutant animals at each larval instar. Rescue of lethality becomes significantly more efficient at older stages. Expression of krzScer\UAS.cRa under the control of Scer\GAL4Kr.PM does not rescue the lethality of krzS047819 animals. Expression of krzScer\UAS.cRa under the control of Scer\GAL4elav-C155 rescues the lethality of krzS047819 animals. Approximately 1/3 of the rescued animals fail to expand their wings after eclosion. Melanotic tumours are visible in the abdomens of most of the rescued animals.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
krzScer\UAS.cRa
krzUAS.cRa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Roman
Secondary FlyBase IDs
    References (4)