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General Information
Symbol
Dmel\krzS047819
Species
D. melanogaster
Name
FlyBase ID
FBal0118174
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
krz1
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{lacW} insertion in an intron.

Insertion components
P{lacW}krzS047819
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Embryos from krzS047819 maternal germline clones show delayed ventral furrow formation and large ventral holes. The same is observed in embryos from krzS047819 homozygous mothers rescued to adulthood by krzVL-A.Tag:SBP.

krzd1/krzS047819 mutant animals survive until the third larval instar, where they become immobile and flaccid and form melanotic tumors. The imaginal discs of these mutant larvae are very reduced in size and display increased cell death.

Expression of krzScer\UAS.cMa in the central nervous system under the control of Scer\GAL4wor.PA rescues the lethality of krzd1/krzS047819. The surviving flies are sterile and display slightly folded wings. Wing discs in rescued larvae have a normal appearance.

49% of krzS047819 mutant cuticles display a large hole either at the anterior or posterior region of the embryo, 31% of mutant embryos display two holes, and 17% of the cuticles display twisting along the anterior-posterior axis. Mutants display gastrulation defects and embryonic lethality.

The embryonic nervous system is disrupted in stage 15 krzS047819 embryos, and animals display holes in the nerve cord, or a twisted nerve cord phenotype.

Homozygous adults which have been rescued from lethality by expression of krzScer\UAS.cRa under the control of Scer\GAL4hs.PB during development (using twice daily heat shocks) die at a faster rate than heterozygous siblings or wild-type controls on medium containing no added NaCl. On medium containing 0.4M NaCl, homozygous adults die at a much faster rate than heterozygous siblings or wild-type controls.

krzS047819 homozygous adults which have been rescued from lethality by expression of krzScer\UAS.cRa under the control of Scer\GAL4hs.PB show a reduced initial activity phase (during the first minute) in an open field arena compared to control flies. The spontaneous locomotor activity is not significantly different from control flies from the second minute onwards (the assay was carried out for 10 minutes).

krzS047819 homozygous adults which have been rescued from lethality by expression of krzScer\UAS.cRa under the control of Scer\GAL4hs.PB and which are also expressing krzScer\UAS.cRa under the control of Scer\GAL4Or83b.T:Hsim\VP16 still show a reduced initial activity phase in an open field arena compared to control flies.

There are no significant differences in spontaneous activity between krzS047819 homozygous adults which have been rescued from lethality by expression of krzScer\UAS.cRa under the control of Scer\GAL4hs.PB and control flies measured in the dark over a period of days.

krzS047819 homozygous adults which have been rescued from lethality by expression of krzScer\UAS.cRa under the control of Scer\GAL4hs.PB fail to show a robust increase in activity after mechanical stimulation, in contrast to control flies.

The majority of homozygous krzS047819 mutants die as embryos, and less than 25% reach the third instar larval stage

Homozygotes die throughout development. Most animals fail to hatch and none of the surviving larvae live to puparium formation. Prior to death the mutant larvae become immobile and flaccid. Third instar larvae develop melanotic tumours, which form throughout the body cavity but most frequently collect in the posterior of the larva. Tumours are frequently found floating free in the haemocoel. Large numbers of lamellocytes are found circulating through the haemocoel of mutant third instar larvae (these cells typically form in wild-type pupae). The fat bodies of mutant larvae shortly after the second moult have a rough appearance compared to those of wild-type larvae. At approximately 18-20 hours after the second moult the fat bodies of surviving mutant larvae begin to dissociate into individual cells (the wild-type fat body does not normally dissociate until after pupariation). Melanotic tumours begin to form on the surface of the mutant fat bodies shortly after the second moult.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference

krzS047819/krz[+] is an enhancer of wing phenotype of Scer\GAL4da.G32, rlSem.S.UAS

krzS047819/krz[+] is an enhancer of wing vein phenotype of Scer\GAL4da.G32, rlSem.S.UAS

krzS047819/krz[+] is an enhancer of eye phenotype of Scer\GAL4da.G32, rlSem.S.UAS

krzS047819/krz[+] is an enhancer of wing margin phenotype of dxS1M

krzS047819/krz[+] is an enhancer of wing vein phenotype of dxS1M

Additional Comments
Genetic Interactions
Statement
Reference

Expression of rlSem.S.Scer\UAS under the control of Scer\GAL4da.G32 in a krzS047819/+ mutant background results in the development of even more additional veins in the wing and a further reduction in wing size.

Expression of rlSem.S.Scer\UAS and krzScer\UAS.T:Zzzz\GS-TAP under the control of Scer\GAL4da.G32 in a krzS047819/+ mutant background restores the wing vein phenotype to that seen in animals expressing just rlSem.S.Scer\UAS under the control of Scer\GAL4da.G32 in a wild-type background. Wing size is larger in these flies compared to animals expressing rlSem.S.Scer\UAS under the control of Scer\GAL4da.G32 in a wild-type background.

The mild irregularities in ommatidial pattern observed in animals expressing rlSem.S.Scer\UAS under the control of Scer\GAL4da.G32 is strongly enhanced in a krzS047819/+ mutant background, and smaller rougher eyes are observed.

Expression of krzScer\UAS.T:Zzzz\GS-TAP restores the regular ommatidial pattern and size of the eye in animals expressing rlSem.S.Scer\UAS under the control of Scer\GAL4da.G32 in a krzS047819/+ mutant background.

The survival rate of animals expressing rlSem.S.Scer\UAS under the control of Scer\GAL4da.G32 drops to 8% in a krzS047819/+ mutant background.

The survival rate of animals expressing rlSem.S.Scer\UAS under the control of Scer\GAL4da.G32 in a krzS047819/+ mutant background increases to 38% when krzScer\UAS.T:Zzzz\GS-TAP is co-expressed.

The notching and vein thickening phenotypes seen in dxS1M hemizygous males are enhanced by krzS047819/+. Survival of NAx-9/NAx-E2 dxENU/+ flies to adulthood is significantly decreased by krzS047819/+.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of krzScer\UAS.cMa in the central nervous system under the control of Scer\GAL4wor.PA rescues the lethality of krzd1/krzS047819. The surviving flies are sterile and display slightly folded wings. Wing discs in rescued larvae have a normal appearance.

Expression of krz+t5.7 in krzS047819 mutant animals fully rescues the embryonic nervous system and cuticule phenotype.

Expression of krzScer\UAS.T:Zzzz\TAP under the control of Scer\GAL4da.G32 in a krzS047819 mutant background completely rescues lethality.

Expression of krzScer\UAS.T:Zzzz\GS-TAP under the control of Scer\GAL4da.G32 in a krzS047819 mutant background completely rescues lethality.

Fully complements the male sterility of mod07570. Lethality is rescued by krz+t12. krz+t5.8 rescues the lethality, formation of melanotic tumours and disaggregation of fat bodies seen in krzS047819 animals. Expression of krzScer\UAS.cRa under the control of Scer\GAL4hs.PB using heat shock is sufficient to rescue some of the krzS047819 mutant animals at each larval instar. Rescue of lethality becomes significantly more efficient at older stages. Expression of krzScer\UAS.cRa under the control of Scer\GAL4Kr.PM does not rescue the lethality of krzS047819 animals. Expression of krzScer\UAS.cRa under the control of Scer\GAL4elav-C155 rescues the lethality of krzS047819 animals. Approximately 1/3 of the rescued animals fail to expand their wings after eclosion. Melanotic tumours are visible in the abdomens of most of the rescued animals.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Complements: l(3)S041303S041303. Complements: l(3)S024314bS024314b. Complements: l(3)S099801bS099801b. Complements: l(3)S134408bS134408b. Complements: l(3)0688606886. Complements: l(3)rH304rH304. Complements: l(3)0649706497. Complements: mod07570. The P{lacW} element is responsible for the lethal phenotype.

ird61 fails to complement the lethality of krzS047819.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (12)