FlyBase Allele Report: Dmel\krz[S095214]

General Information

Symbol

Dmel\krz^S095214

Species

D. melanogaster

Name

FlyBase ID

FBal0099971

Feature type

allele

Associated gene

Dmel\krz

Associated Insertion(s)

P{lacW}krz^S095214

Carried in Construct

Also Known As

krz²

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Deak et al., 1997)

P-element activity

(Szeged Stock Center, 1998-)

Progenitor genotype

Associated Insertion(s)

P{lacW}krz^S095214

Cytology

Description

P{lacW} insertion approximately 60kb upstream of the krz locus.

(Roman et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Roman et al., 2000, Szeged Stock Center, 1998-)

melanotic mass phenotype | larval stage | recessive

(Roman et al., 2000)

Phenotype Manifest In

embryonic/larval fat body

(Roman et al., 2000)

melanotic mass | ectopic

(Roman et al., 2000)

Detailed Description

Statement

Reference

Homozygotes die throughout development. Most animals fail to hatch and none of the surviving larvae live to puparium formation. The formation of melanotic tumours and fat body disaggregation is identical to that seen in krz^S047819 animals. Tumours form throughout the body cavity but most frequently collect in the posterior of the larva. Tumours are frequently found floating free in the haemocoel.

(Roman et al., 2000)

Lethality acts in the second larval instar.

(Deak et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

krz[+]/krz^S095214 is an enhancer of visible | recessive phenotype of dx^S1M

(Mukherjee et al., 2005)

krz^S095214 is an enhancer of partially lethal - majority die phenotype of N^Ax-9/N^Ax-E2, dx^ENU/dx[+]

(Mukherjee et al., 2005)

Phenotype Manifest In

Enhancer of

Statement

Reference

krz[+]/krz^S095214 is an enhancer of wing margin phenotype of dx^S1M

(Mukherjee et al., 2005)

krz[+]/krz^S095214 is an enhancer of wing vein phenotype of dx^S1M

(Mukherjee et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The notching and vein thickening phenotypes seen in dx^S1M hemizygous males are enhanced by krz^S095214/+. Survival of N^Ax-9/N^Ax-E2 dx^ENU/+ flies to adulthood is significantly decreased by krz^S095214/+.

(Mukherjee et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

krz⁴

(Roman et al., 2000)

Fails to complement

krz³

(Roman et al., 2000)

krz^L8

(Roman et al., 2000)

krz^S047819

(Roman et al., 2000)

Rescued by

krz^S095214 is rescued by krz^+t12

(Roman et al., 2000)

Comments

Lethality is rescued by krz^+t12.

(Roman et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

It is possible that an undetected second site mutation at the krz locus and not the P{lacW}krz^S095214 insertion (which maps approximately 60kb upstream of the krz locus) disrupts the expression of krz. However, no polymorphisms have been detected by restriction analysis within a 12kb SphI fragment that includes both mod and krz, and no sequence changes in approximately 4.5kb of the krz gene and promoter region have been detected in krz^S095214 mutants.

(Roman et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

0952/14

(Deak et al., 1997)

krz²

(Mukherjee et al., 2005, Roman et al., 2000)

krz^S095214

l(3)95214

(Roman et al., 2000)

l(3)S095214^S095214

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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