FlyBase Allele Report: Dmel\crc[R6]

General Information

Symbol

Dmel\crc^R6

Species

D. melanogaster

Name

FlyBase ID

FBal0118283

Feature type

allele

Associated gene

Dmel\crc

Associated Insertion(s)

P{?GawB}crc^R6

Carried in Construct

Also Known As

R6-gal4, R6, atf4^R6

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Hewes et al., 2000)

Progenitor genotype

P{GawB}crc⁹²⁹

(Hewes et al., 2000)

Associated Insertion(s)

P{?GawB}crc^R6

Caused by aberration

Df(2L)crc^R6

Cytology

Description

Imprecise excision of the P{GawB} element, resulting in a deletion removing crc exons that encode the bZIP domain. This mutation disrupts crc isoforms A and B. Some or all of the P{GawB} element remains.

(Hewes et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Hewes et al., 2000)

Phenotype Manifest In

adult head

(Hewes et al., 2000)

anterior pupal spiracle

(Hewes et al., 2000)

cephalopharyngeal skeleton (with Df(2L)Rev8)

(Hewes et al., 2000)

leg disc

(Hewes et al., 2000)

pupa

(Hewes et al., 2000)

wing disc

(Hewes et al., 2000)

Detailed Description

Statement

Reference

Mutants show several lethal phases during larval, pupal and adult development. Hemizygotes show 15-50% of their lethality after pupariation. Hemizygous puparia are generally aberrant to varying degrees (approximately 5% are indistinguishable from wild type). The defects include a failure to evert the anterior spiracles, and a retention of a larval shape, which is thinned, elongated and sometimes curved to one side. In the most severe cases, the abdominal gas bubble, which normally forms approximately 6 hours after pupariation, does not appear, although the larval mouthparts are later expelled. Head eversion fails or is incomplete and the leg and wing discs do not completely elongate. Third instar crc^R6/Df(2L)Rev8 larvae with multiple mouthparts are seen. crc^R6 shows the "head/abdomen-collapsed" phenotype when heterozygous over some second chromosome balancers, suggesting haploinsufficiency for crc in some genetic backgrounds.

(Hewes et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

crc¹/crc^R6 is a non-suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, ninaE^G69D.UAS

(Kang et al., 2012)

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

crc¹/crc^R6 is a non-suppressor of eye disc phenotype of Scer\GAL4^GMR.PF, ninaE^G69D.UAS

(Kang et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The massive apoptosis seen in the larval eye discs of animals expressing ninaE^{G69D.Scer\UAS} under the control of Scer\GAL4^GMR.PF is not suppressed by crc^R6/crc¹.

(Kang et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

crc^E85

(Hewes et al., 2000)

Fails to complement

crc^E98

(Hewes et al., 2000)

crc^R1