FlyBase Allele Report: Dmel\nuf[EP3324]

General Information

Symbol

Dmel\nuf^EP3324

Species

D. melanogaster

Name

FlyBase ID

FBal0119171

Feature type

allele

Associated gene

Dmel\nuf

Associated Insertion(s)

P{EP}nuf^EP3324

Carried in Construct

Also Known As

EP(3)3324

Key Links

Genomic Maps

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Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Bidet et al., 2003, Gene Disruption Project members, 2001-, Abdelilah-Seyfried et al., 2000)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:14,205,377..14,205,377 [+]

(Emery et al., 2005, Bidet et al., 2003, Gene Disruption Project members, 2001-, Abdelilah-Seyfried et al., 2000)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

An P{EP} insertion 54bp upstream of an alternative start of the nuf gene.

(Emery et al., 2005)

P{EP} insertion, oriented such that expression from the element should result in overexpression of nuf.

(Bidet et al., 2003)

Insertion components

P{EP}nuf^EP3324

Product class / Tool use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

misexpression element

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^sca-537.4

(Abdelilah-Seyfried et al., 2000)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of nuf^EP3324 under the control of Scer\GAL4^sca.PU in the adult thorax leads to an increase in the frequency of bristle twinning, as compared to controls.

(Karbowniczek et al., 2010)

Expression of nuf^EP3324 under the control of Scer\GAL4^sca-109-68 at 29^oC leads to the production of external sensory organs with multiple sockets and no hair in ~9% of cases.

(Emery et al., 2005)

Expression of nuf^EP3324 under the control of Scer\GAL4^sca-537.4 results in additional scutellar macrochaetae. Sensory organs with one socket and two shafts are seen.

(Abdelilah-Seyfried et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^sca-109-68, nuf^EP3324 has trichogen cell phenotype, enhanceable by Rab11^QL.UAS, Scer\GAL4^sca-109-68

(Emery et al., 2005)

Scer\GAL4^sca-109-68, nuf^EP3324 has tormogen cell | increased number phenotype, enhanceable by Rab11^QL.UAS, Scer\GAL4^sca-109-68

(Emery et al., 2005)

Scer\GAL4^sca-109-68, nuf^EP3324 has trichogen cell phenotype, enhanceable by l(2)gl^3A.UAS/Scer\GAL4^sca-109-68

(Emery et al., 2005)

Scer\GAL4^sca-109-68, nuf^EP3324 has tormogen cell | increased number phenotype, enhanceable by l(2)gl^3A.UAS/Scer\GAL4^sca-109-68

(Emery et al., 2005)

Enhancer of

Statement

Reference

nuf^EP3324, Scer\GAL4^sca-109-68 is an enhancer of trichogen cell phenotype of Rab11^QL.UAS, Scer\GAL4^sca-109-68

(Emery et al., 2005)

nuf^EP3324, Scer\GAL4^sca-109-68 is an enhancer of tormogen cell phenotype of Rab11^QL.UAS, Scer\GAL4^sca-109-68

(Emery et al., 2005)

Scer\GAL4^sca-109-68/nuf^EP3324 is an enhancer of external sensory organ precursor cell IIb phenotype of Scer\GAL4^sca-109-68, l(2)gl^3A.UAS

(Emery et al., 2005)

Scer\GAL4^sca-109-68/nuf^EP3324 is an enhancer of external sensory organ precursor cell IIa phenotype of Scer\GAL4^sca-109-68, l(2)gl^3A.UAS

(Emery et al., 2005)

NOT Enhancer of

Statement

Reference

nuf^EP3324, Scer\GAL4^sca.PU is a non-enhancer of mesothoracic bristle | increased number | adult stage phenotype of Rheb^UAS.cPa, Scer\GAL4^sca.PU

(Karbowniczek et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of nuf^EP3324 does not enhance the bristle twinning phenotype of flies expressing Rheb^Scer\UAS.cPa under the control of Scer\GAL4^sca.PU.

(Karbowniczek et al., 2010)

Sensory organ precursor cells expressing l(2)gl^3A.Scer\UAS and nuf^EP3324 under the control of Scer\GAL4^sca-109-68 show an enhancement of the pIIb to pIIa transformation seen when only l(2)gl^3A.Scer\UAS is expressed and an enhancement of the hair to socket transformation seen when only nuf^EP3324 is expressed.

Coexpression of Rab11^QL.Scer\UAS and nuf^EP3324, under the control of Scer\GAL4^sca-109-68, leads to an enhancement of the hair to socket transformations seen when either transgene is expressed alone.

(Emery et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

EP(3)3324

(Abdelilah-Seyfried et al., 2000)

nuf^EP3324

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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