FlyBase Allele Report: Dmel\Fmr1[UAS.cWa]

General Information

Symbol

Dmel\Fmr1^UAS.cWa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Wan

FlyBase ID

FBal0120821

Feature type

allele

Associated gene

Dmel\Fmr1

Associated Insertion(s)

Carried in Construct

P{UAS-Fmr1.W}

Also Known As

UAS-dfmr1, UAS-Fmr1

Key Links

Transgenic product class

wild_type

(Wan et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Wan et al., 2000)

Mutagen

in vitro construct

(Wan et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-Fmr1.W}

Cytology

Description

UAS sequences drive expression of the Fmr1 open reading frame.

(Wan et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Fmr1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of fragile X syndrome

CEA

(Bienkowski et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of fragile X syndrome

is exacerbated by Nab2^EP3716

(Bienkowski et al., 2017)

exacerbates autosomal recessive intellectual developmental disorder

modeled by Nab2^GD11745

(Bienkowski et al., 2017)

exacerbates neurodegenerative disease

modeled by Zzzz\GGGGCC^48.UAS

(Schweizer Burguete et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav-C155

(Bienkowski et al., 2017)

abnormal locomotor behavior | larval stage, with Scer\GAL4^109(2)80

(Xu et al., 2004)

increased cell death, with Scer\GAL4^hs.2sev

(Wan et al., 2000)

Phenotype Manifest In

anterior crossvein, with Scer\GAL4^dpp.blk1

(Wan et al., 2000)

dendritic arborizing neuron, with Scer\GAL4^109(2)80

(Lee et al., 2003)

eye, with Scer\GAL4^hs.2sev

(Wan et al., 2000)

eye photoreceptor cell, with Scer\GAL4^hs.2sev

(Wan et al., 2000)

ommatidium, with Scer\GAL4^hs.2sev

(Wan et al., 2000)

pigment cell, with Scer\GAL4^hs.2sev

(Wan et al., 2000)

rhabdomere, with Scer\GAL4^hs.2sev

(Wan et al., 2000)

wing, with Scer\GAL4^dpp.blk1

(Wan et al., 2000)

wing, with Scer\GAL4^vg.PM

(Wan et al., 2000)

wing cell, with Scer\GAL4^dpp.blk1

(Wan et al., 2000)

wing cell, with Scer\GAL4^vg.PM

(Wan et al., 2000)

wing margin, with Scer\GAL4^vg.PM

(Wan et al., 2000)

Detailed Description

Statement

Reference

Scer\GAL4^elav-C155-driven expression of Fmr1^Scer\UAS.cWa leads to adult locomotion deficit (reduced climbing ability) compared to controls.

(Bienkowski et al., 2017)

Expression of Fmr1^Scer\UAS.cWa under the control of Scer\GAL4^109(2)80 results in defects in crawling behaviour in wandering larvae; the larvae show an increase in the time percentage spent on linear locomotion compared to controls and the duration of the linear locomotion is increased. The average direction change (direction change is defined as the difference in direction from one frame to the next in the analysis) is reduced compared to controls.

(Xu et al., 2004)

When Fmr1^{Scer\UAS.T:Avic\GFP} is driven by Scer\GAL4^109(2)80 the number of terminal dendritic processes in both the ventral and dorsal dendritic arborising neurons (DAs). The lengths of the remining terminal processes is also greatly reduced. This phenotype is 100% penetrant. The major dendritic branches are still capable of extending more than fivefold during larval development, however most terminal processes fail to form or extend even at the first instar stage.

(Lee et al., 2003)

Over expression of Fmr1^Scer\UAS.cWa when driven by Scer\GAL4^dpp.blk1 causes cell loss in the wing, a decrease in cells in seen between longitudinal veins 3 and 4, as well as loss of the anterior crossvein. Over expression of Fmr1^Scer\UAS.cWa when driven by Scer\GAL4^vg.PM causes cell loss in the wing - cell loss at the wing margin is seen with 100% penetrance. Expression of Fmr1^Scer\UAS.cWa in the eye disc when driven by Scer\GAL4^hs.2sev causes an increase in apoptotic cell death behind the morphogenetic furrow. The adult eye lack some photoreceptor cells and pigment cells. Overexpression also leads to the alteration of rhabdomere shape.

(Wan et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^ey-OK107/Fmr1^UAS.cWa is an enhancer of abnormal neuroanatomy | adult stage phenotype of Nab2^ex3

(Bienkowski et al., 2017)

Fmr1^UAS.cWa, Scer\GAL4^elav-C155 is an enhancer of abnormal locomotor behavior | adult stage phenotype of Nab2^GD11745, Scer\GAL4^elav-C155

(Bienkowski et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Fmr1^UAS.cWa, Scer\GAL4^109(2)80 has dendritic arborizing neuron phenotype, suppressible | partially by Rac1^UAS.Tag:MYC, Scer\GAL4^109(2)80

(Lee et al., 2003)

Enhancer of

Statement

Reference

Scer\GAL4^ey-OK107/Fmr1^UAS.cWa is an enhancer of mushroom body | adult stage phenotype of Nab2^ex3

(Bienkowski et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The mushroom body morphology defects in Nab2^ex3 adult mutants are exacerbated by Scer\GAL4^ey-OK107-driven expression of Fmr1^Scer\UAS.cWa.

The locomotion deficit (decreased climbing ability) characteristic for adult flies expressing Nab2^GD11745 under the control of Scer\GAL4^elav-C155 is worsened further by co-expression of Fmr1^Scer\UAS.cWa (which on its own also induces climbing ability defects).

(Bienkowski et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Mef2.247/Fmr1^UAS.cWa rescues Fmr1³