FlyBase Allele Report: Dmel\scb[Vol-4]

General Information

Symbol

Dmel\scb^Vol-4

Species

D. melanogaster

Name

FlyBase ID

FBal0121535

Feature type

allele

Associated gene

Dmel\scb

Associated Insertion(s)

Carried in Construct

Also Known As

vol⁴

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Rohrbough et al., 2000)

Mutagen

P-element activity

(Rohrbough et al., 2000)

Progenitor genotype

scb^Vol-1

(Rohrbough et al., 2000)

Cytology

Description

Deletion of 1640bp, including the first 118bp of exon 2 and 1522 bp of the preceding intron.

(Rohrbough et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage

(Rohrbough et al., 2000)

abnormal neurophysiology | larval stage

(Rohrbough et al., 2000)

lethal - all die before end of larval stage | recessive

(Rohrbough et al., 2000)

some die during larval stage | recessive

(Rohrbough et al., 2000)

Phenotype Manifest In

axon terminus | increased number

(Rohrbough et al., 2000)

bouton | increased number

(Rohrbough et al., 2000)

neuromuscular junction

(Rohrbough et al., 2000)

Detailed Description

Statement

Reference

Embryos hatch normally but show locomotor defects in the larval stages. Most die during the second or third instar, few pupate and non survive to adult stages. Nmjs show no obvious defects in neuronal pathfinding or synaptogenesis. At hatching CNS morphology, peripheral nerve branching, neuromuscular patterning and nmj distribution all appear normal. However, starting at mid-2nd instar, larvae begin to show nmj overgrowth: terminals are enlarged with 60-100% more synaptic terminal branches and 30-40% more synaptic boutons than controls. At physiological levels of external Ca²⁺ mutant nmjs show normal transmission. At reduced external Ca²⁺ levels EJC amplitudes are increased over those of controls (least pronounced for scb^Vol-2, but highly significant for scb^Vol-1, scb^Vol-3 and scb^Vol-4. scb^Vol-1, scb^Vol-3 and scb^Vol-4 show reduced slope of the logarithmic relationship between EJC amplitude and external Ca²⁺ concentration, indicating reduced Ca²⁺ dependence of transmission. mEJC variability, amplitude (indicative of postsynaptic glutamate receptor density) is indistinguishable from wild type. Mutants show significant defects in two forms of short-term facilitation, paired pulse and frequency dependent short term facilitation. Mutants show impaired augmentation of transmission amplitude upon prolonged stimulation at intermediate frequencies.

(Rohrbough et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

scb^Vol-4 is a non-enhancer of abnormal neuroanatomy phenotype of RhoGAPp190^RNAi.GAP.UAS, Scer\GAL4^ey-OK107

(Billuart et al., 2001)

NOT Suppressor of

Statement

Reference

scb^Vol-4 is a non-suppressor of abnormal neuroanatomy phenotype of RhoGAPp190^RNAi.GAP.UAS, Scer\GAL4^ey-OK107

(Billuart et al., 2001)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

scb^Vol-4 is a non-enhancer of mushroom body vertical lobe phenotype of RhoGAPp190^RNAi.GAP.UAS, Scer\GAL4^ey-OK107

(Billuart et al., 2001)

NOT Suppressor of

Statement

Reference

scb^Vol-4 is a non-suppressor of mushroom body vertical lobe phenotype of RhoGAPp190^RNAi.GAP.UAS, Scer\GAL4^ey-OK107

(Billuart et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

scb^Vol-4 is partially rescued by scb^s.hs

(Rohrbough et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Vol⁴

(Rohrbough et al., 2000)

scb^Vol-4

vol⁴

(Billuart et al., 2001)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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