ph-p504 mutant cell clones cause overgrowth in the wing disc.
Enbryos from ph-p504 heterozygous mothers show segregation defects at anaphase and telophase as judged by the presence of chromatin bridges. Most of these chromatin bridges are probably resolved because large irregularly-shaped nuclei and polyploid nuclei are only seen at a low penetrance. ph-p504 embryos show a higher level of "nuclear fallout", a process that removes nuclei with abnormal mitoses, than wild-type embryos. Fallout nuclei tend to be observed in pairs or clusters and have a late telophase appearance. 2% of these embryos show a "mitotic catastrophe" phenotype, characterized by completely disorganized chromosomes that have no regular pattern or normal ploidy and very few, abnormally large nuclei that may be amorphous or may have a normal, spherical shape. There is no evidence of metaphase defects in these embryos although a small number show chromatin bridges that persist to the prometaphase stage.
ph-d504, ph-p504 has egg chamber | somatic clone phenotype
ph-d504, ph-p504 has stalk follicle cell | somatic clone phenotype
ph-d504, ph-p504 has germarium | somatic clone phenotype
ph-d504, ph-p504 has wing disc | somatic clone phenotype
Ovaries carrying germ-line clones homozygous for "ph0" (ph-d504 ph-p504) are normal. When somatic clones homozygous for "ph0" (ph-d504 ph-p504) are induced 4, 6 or 8 days after eclosion, they are rarely detected in the follicular epithelium of the ovary even though large wild-type twin-spots are frequently present. These mutant clones can occasionally be observed in the process of being 'expulsed' from the follicular epithelium. Despite this, ovaries containing these clones contain fused egg chambers and apposed egg chambers not separated by an interfollicular stalk. Where stalks are present they are sometimes abnormal - consisting of more than a dozen cells in 2-3 lines rather than five to seven cells organized in a single line as in wild-type. Only some of these abnormal stalks contain (surviving) mutant cells. Polar follicle cells are generally unaffected, although additional polar cells are very occasionally present in egg chambers of stage 5 or later. Small somatic clones of "ph0" (ph-d504 ph-p504) homozygous cells can be recovered in the germarium. In region 2b/3 of the germarium, these cells fail to extend long, thin cell processes centripetally as their wild-type counterparts do, but instead remain at the periphery with an abnormally rounded morphology.
ph-p504 is rescued by ph-pUAS.Pc.GFP/Scer\GAL4unspecified