FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\ph-p504
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General Information
Symbol
Dmel\ph-p504
Species
D. melanogaster
Name
FlyBase ID
FBal0122936
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ph504, ph0
Key Links
Mutagen
    Nature of the Allele
    Mutagen
    Progenitor genotype
    Cytology
    Description
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    Variant Molecular Consequences
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    ph-p504 mutant cell clones cause overgrowth in the wing disc.

    Enbryos from ph-p504 heterozygous mothers show segregation defects at anaphase and telophase as judged by the presence of chromatin bridges. Most of these chromatin bridges are probably resolved because large irregularly-shaped nuclei and polyploid nuclei are only seen at a low penetrance. ph-p504 embryos show a higher level of "nuclear fallout", a process that removes nuclei with abnormal mitoses, than wild-type embryos. Fallout nuclei tend to be observed in pairs or clusters and have a late telophase appearance. 2% of these embryos show a "mitotic catastrophe" phenotype, characterized by completely disorganized chromosomes that have no regular pattern or normal ploidy and very few, abnormally large nuclei that may be amorphous or may have a normal, spherical shape. There is no evidence of metaphase defects in these embryos although a small number show chromatin bridges that persist to the prometaphase stage.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    Ovaries carrying germ-line clones homozygous for "ph0" (ph-d504 ph-p504) are normal. When somatic clones homozygous for "ph0" (ph-d504 ph-p504) are induced 4, 6 or 8 days after eclosion, they are rarely detected in the follicular epithelium of the ovary even though large wild-type twin-spots are frequently present. These mutant clones can occasionally be observed in the process of being 'expulsed' from the follicular epithelium. Despite this, ovaries containing these clones contain fused egg chambers and apposed egg chambers not separated by an interfollicular stalk. Where stalks are present they are sometimes abnormal - consisting of more than a dozen cells in 2-3 lines rather than five to seven cells organized in a single line as in wild-type. Only some of these abnormal stalks contain (surviving) mutant cells. Polar follicle cells are generally unaffected, although additional polar cells are very occasionally present in egg chambers of stage 5 or later. Small somatic clones of "ph0" (ph-d504 ph-p504) homozygous cells can be recovered in the germarium. In region 2b/3 of the germarium, these cells fail to extend long, thin cell processes centripetally as their wild-type counterparts do, but instead remain at the periphery with an abnormally rounded morphology.

    Homozygous "ph0" (ph-d504 ph-p504) clones in the wing disc are large in size and rounded in shape and the imaginal disc cells are larger than wild type.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer

    Associated with: ph-p504. The "ph504" chromosome carries mutations in both ph-d and ph-p.

    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (5)
    References (13)