FlyBase Allele Report: Dmel\Tsc1[UAS.cGa]

General Information

Symbol

Dmel\Tsc1^UAS.cGa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Gao

FlyBase ID

FBal0123963

Feature type

allele

Associated gene

Dmel\Tsc1

Associated Insertion(s)

Carried in Construct

P{UAS-Tsc1.G}

Also Known As

UAS-TSC1

Key Links

Transgenic product class

cDNA

(Gao and Pan, 2001)

wild_type

(Gao and Pan, 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Gao and Pan, 2001)

wild_type

(Gao and Pan, 2001)

Mutagen

in vitro construct

(Gao and Pan, 2001)

Progenitor genotype

Carried in construct

P{UAS-Tsc1.G}

Cytology

Description

UAS sequences drive expression of the Tsc1 cDNA.

(Gao and Pan, 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Tsc1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurodegenerative disease

modeled by trp^P365

(Huang et al., 2016)

ameliorates Huntington's disease

modeled by Hsap\HTT^GMR.Q120

(Wang et al., 2009)

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

Co-expression (single expression of either construct untested) of Tsc1^Scer\UAS.cGa and gig^Scer\UAS.cTa ameliorates neurodegenerative disease modeled by trp^P365.

(Huang et al., 2016)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

long lived, with Scer\GAL4^da.G32

(Kapahi et al., 2004)

wild-type, with Scer\GAL4^Bx-MS1096

(Gao and Pan, 2001)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expression of Tsc1^Scer\UAS.cGa under the control of Scer\GAL4^da.G32 at 29^oC results in an increase in mean life span of 14% compared to control flies.

(Kapahi et al., 2004)

Expression of Tsc1^Scer\UAS.cGa when driven by Scer\GAL4^Bx-MS1096 causes no visible phenotype.

(Gao and Pan, 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.long, Tsc1^UAS.cGa, gig^UAS.cTa has abnormal size | adult stage phenotype, non-enhanceable by TBC1d7^UAS.cRa/Scer\GAL4^GMR.long

(Ren et al., 2018)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.long, Tsc1^UAS.cGa, gig^UAS.cTa has abnormal size | adult stage phenotype, non-suppressible by TBC1d7^UAS.cRa/Scer\GAL4^GMR.long

(Ren et al., 2018)

Suppressor of

Statement

Reference

gig^UAS.cTa, Scer\GAL4^GMR.PF, Tsc1^UAS.cGa is a suppressor of abnormal neuroanatomy | dominant | adult stage | progressive phenotype of trp^P365

(Huang et al., 2016)

Tsc1^UAS.cGa/Scer\GAL4^GMR.PU is a suppressor of increased cell death phenotype of norpA^P24

(Wang et al., 2009)

gig^UAS.cTa, Scer\GAL4^arm.PS, Tsc1^UAS.cGa is a suppressor | partially of lethal | late third instar larval stage phenotype of Df(2L)flp170B/Pten³

(Vereshchagina et al., 2008)

Other

Statement

Reference

Scer\GAL4^GMR.long, Tsc1^UAS.cGa, gig^UAS.cTa has abnormal size | adult stage phenotype

(Ren et al., 2018)

Scer\GAL4^da.G32, Tsc1^UAS.cGa, gig^UAS.cTa has lethal phenotype

(Kapahi et al., 2004)

Scer\GAL4^Bx-MS1096, Tsc1^UAS.cGa, gig^UAS.cTa has decreased cell size phenotype

(Gao and Pan, 2001)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.long, Tsc1^UAS.cGa, gig^UAS.cTa has ommatidium phenotype, non-enhanceable by TBC1d7^UAS.cRa/Scer\GAL4^GMR.long

(Ren et al., 2018)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.long, Tsc1^UAS.cGa, gig^UAS.cTa has ommatidium phenotype, non-suppressible by TBC1d7^UAS.cRa/Scer\GAL4^GMR.long

(Ren et al., 2018)

NOT Enhancer of

Statement

Reference

Tsc1^UAS.cGa/Scer\GAL4^GMR.PU is a non-enhancer of photoreceptor neuron phenotype of ninaE^RH27

(Wang et al., 2009)

Suppressor of

Statement

Reference

gig^UAS.cTa, Scer\GAL4^GMR.PF, Tsc1^UAS.cGa is a suppressor of rhabdomere of eye photoreceptor cell | adult stage | progressive phenotype of trp^P365

(Huang et al., 2016)

gig^UAS.cTa, Tsc1^UAS.cGa, Scer\GAL4^GMR.PU is a suppressor of photoreceptor neuron phenotype of norpA^P24

(Wang et al., 2009)

gig^UAS.cTa, Tsc1^UAS.cGa, Scer\GAL4^GMR.PU is a suppressor of retina phenotype of norpA^P24

(Wang et al., 2009)

Other

Statement

Reference

Scer\GAL4^GMR.long, Tsc1^UAS.cGa, gig^UAS.cTa has ommatidium phenotype

(Ren et al., 2018)

Scer\GAL4^Bx-MS1096, Tsc1^UAS.cGa, gig^UAS.cTa has wing phenotype

(Gao and Pan, 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The co-expression of Tsc1^UAS.cGa and gig^UAS.cTa under the control of Scer\GAL4^GMR.long leads to a small but significant decrease in ommatidia area, as compared to controls.

(Ren et al., 2018)

Co-expression of Tsc1^Scer\UAS.cGa and gig^Scer\UAS.cTa driven by Scer\GAL4^GMR.PF (to induce autophagy) partially suppresses age-dependent retinal degeneration (loss of rhabdomeres from photoreceptors) in trp^P365/+ flies.

(Huang et al., 2016)

norpA³⁶ -mediated photoreceptor degeneration is significantly suppressed by overexpression of Tsc1^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PU.

Overexpression of Tsc1^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PU does not attenuate photoreceptor cell death in ninaE^RH27 flies and even has converse effects.

(Wang et al., 2009)

Expression of Tsc1^Scer\UAS.cGa and gig^Scer\UAS.cGa under the control of Scer\GAL4^arm.PS rescues lethality in 40% of Pten³/Df(2L)flp170B animals.

(Vereshchagina et al., 2008)

Co-expression of gig^Scer\UAS.cGa and Tsc1^Scer\UAS.cGa when driven by Scer\GAL4^Bx-MS1096 leads to a dramatic reduction in wing size. There is a 72% reduction in wing size and a 65% reduction in cell size.

(Gao and Pan, 2001)

Xenogenetic Interactions

Statement

Reference

Overexpression of Tsc1^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PU largely suppresses neural degeneration caused by expression of Hsap\HD^GMR.Q120.

(Wang et al., 2009)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

80963

w^*; P{UAS-Tsc1.G}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Tsc1^Scer\UAS.cGa

Tsc1^UAS.cGa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Gao

(Gao and Pan, 2001)

Secondary FlyBase IDs

References (8)

Report Sections

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