FlyBase Allele Report: Dmel\tefu[Δ356]

General Information

Symbol

Dmel\tefu^Δ356

Species

D. melanogaster

Name

FlyBase ID

FBal0124385

Feature type

allele

Associated gene

Dmel\tefu

Associated Insertion(s)

Carried in Construct

Genomic Maps

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Allele class

hypomorphic allele - genetic evidence

loss of function allele

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Song et al., 2004)

loss of function allele

(Oikemus et al., 2004)

Mutagen

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

deletion

3R:15,238,332..15,247,104 [-]

Comment:

Imprecise excision of the EP(3)0859 P-element, deleting 8773 nucleotides, including the first 8 coding exons of tefu. Position of mutation on reference sequence inferred by FlyBase curator.

(Song et al., 2004)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Deletion breakpoint within tefu. The deletion removes the first 8 exons of tefu.

(Song et al., 2004)

Caused by aberration

Df(3R)Hsc70-4^Δ356

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of ataxia telangiectasia

CEA with tefu¹

(Oikemus et al., 2004)

CEA with Hsc70-4^+t14

(Oikemus et al., 2004, Song et al., 2004)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of ataxia telangiectasia

is ameliorated by p53^R155H.UAS.Ex

(Song et al., 2004)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

female sterile | recessive

(Song et al., 2004)

increased cell death

(Oikemus et al., 2004, Song et al., 2004)

lethal - all die before end of pupal stage | recessive

(Oikemus et al., 2004, Song et al., 2004)

mitotic cell cycle defective

(Song et al., 2004)

mitotic cell cycle defective (with tefu¹)

(Oikemus et al., 2006)

some die during pupal stage | recessive

(Oikemus et al., 2004, Song et al., 2004)

Phenotype Manifest In

chromosome, telomeric region

(Song et al., 2004)

eye

(Oikemus et al., 2004)

eye (with Df(3R)PG4)

(Oikemus et al., 2004)

eye (with tefu¹)

(Oikemus et al., 2004)

eye disc

(Song et al., 2004)

macrochaeta

(Oikemus et al., 2004)

macrochaeta (with Df(3R)PG4)

(Oikemus et al., 2004)

macrochaeta (with tefu¹)

(Oikemus et al., 2004)

mitosis & nuclear chromosome

(Song et al., 2004)

wing

(Oikemus et al., 2004, Song et al., 2004)

wing (with Df(3R)PG4)

(Oikemus et al., 2004)

wing (with tefu¹)

(Oikemus et al., 2004)

wing disc

(Oikemus et al., 2004, Song et al., 2004)

Detailed Description

Statement

Reference

tefu¹/tefu^Δ356 mutant wing discs exhibit a partial arrest phenotype at low doses of irradiation.

(Oikemus et al., 2006)

tefu^Δ356 animals (created by combining Df(3R)Hsc70-4^Δ356 and Hsc70-4^+t14 to rescue defects cause by the disruption of the Hsc70-4^+t14 gene in that Deficiency) die shortly before eclosion. They exhibit a rough eye phenotype, misshapen wings and missing or abnormal bristles. Similar effects are see when combined with Df(3R)PG4 also. tefu¹/tefu^red31 animals exhibit rough eyes, notched wings, and shorter or missing bristles, but do survive to adulthood. Mutant wing discs exhibit extremely high levels of spontaneous apoptosis, particularly in the basal region of the disc.

(Oikemus et al., 2004)

Most tefu^Δ356 homozygous flies (Df(3R)Hsc70-4^Δ356 homozygotes in which the lack of Hsc70-4 function has been rescued by Hsc70-4^+t14) show pupal lethality, although 15% eclose and are viable. These adult escapers do not survive for very long after eclosion. Escaper females do not lay eggs. Adult escapers have a notched wing phenotype. Third larval instar tefu^Δ356 wing and eye discs (from Df(3R)Hsc70-4^Δ356 animals in which the lack of Hsc70-4 function has been rescued by Hsc70-4^+t14) show a substantial increase in the number of apoptotic cells compared to wild-type discs. Mitotic neuroblasts from tefu^Δ356 brains (from Df(3R)Hsc70-4^Δ356 animals in which the lack of Hsc70-4 function has been rescued by Hsc70-4^+t14) often show evidence of chromosomal damage; in particular, telomere fusions are seen in 65.5% of metaphase figures. Both single and double telomere associations are seen, including fusions between sister chromatids as well as between homologous and nonhomologous chromosomes.

(Song et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

tefu^Δ356 has increased cell death phenotype, suppressible | partially by p53^11-1B-1

(Oikemus et al., 2004)

tefu^Δ356 has increased cell death phenotype, suppressible by Scer\GAL4^en-e16E/p53^R155H.UAS.Ex

(Song et al., 2004)

NOT suppressed by

Statement

Reference

tefu^Δ356 has lethal | recessive | pupal stage phenotype, non-suppressible by p53^11-1B-1

(Oikemus et al., 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

tefu^Δ356 has wing disc phenotype, suppressible | partially by p53^11-1B-1

(Oikemus et al., 2004)

tefu^Δ356 has wing disc phenotype, suppressible by Scer\GAL4^en-e16E/p53^R155H.UAS.Ex

(Song et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of p53^{R155H.Scer\UAS.Ex} under the control of Scer\GAL4^en-e16E results in a substantial decrease in apoptosis (in the domain of Scer\GAL4^en-e16E expression) in third larval instar tefu^Δ356 wing discs (from Df(3R)Hsc70-4^Δ356 animals in which the lack of Hsc70-4 function has been rescued by Hsc70-4^+t14).

(Song et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

tefu¹

(Oikemus et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG6535^Δ356

(Bronk et al., 2001)

atm^Δ356

(Oikemus et al., 2004)

tefu^Δ356

(Oikemus et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (4)

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