FlyBase Allele Report: Dmel\psidin[1]

General Information

Symbol

Dmel\psidin¹

Species

D. melanogaster

Name

FlyBase ID

FBal0127967

Feature type

allele

Associated gene

Dmel\psidin

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Brennan et al., 2007)

Mutagen

ethyl methanesulfonate

(Wu et al., 2001)

Progenitor genotype

Cytology

Description

Amino acid replacement: K441term.

(Brennan et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:20,024,521..20,024,521 [+]

Nucleotide change:

A20024521T

Amino acid change:

K441term | psidin-PA

Reported amino acid change:

K441term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Brennan et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | recessive

(Wu et al., 2001)

abnormal neuroanatomy | adult stage | somatic clone

(Stephan et al., 2012)

lethal | recessive

(Wu et al., 2001)

lethal - all die before end of larval stage (with Df(3R)H15)

(Brennan et al., 2007)

melanotic mass phenotype

(Wu et al., 2001)

some die during third instar larval stage (with Df(3R)H15)

(Brennan et al., 2007)

Phenotype Manifest In

adult olfactory receptor neuron Or10a | somatic clone

(Stephan et al., 2012)

adult olfactory receptor neuron Or22 | somatic clone

(Stephan et al., 2012)

adult olfactory receptor neuron Or33c/85e | somatic clone

(Stephan et al., 2012)

adult olfactory receptor neuron Or42a | somatic clone

(Stephan et al., 2012)

adult olfactory receptor neuron Or46aA | somatic clone

(Stephan et al., 2012)

adult olfactory receptor neuron Or47a | somatic clone

(Stephan et al., 2012)

adult olfactory receptor neuron Or47b | somatic clone

(Stephan et al., 2012)

adult olfactory receptor neuron Or59c | somatic clone

(Stephan et al., 2012)

adult olfactory receptor neuron Or71a | somatic clone

(Stephan et al., 2012)

adult olfactory receptor neuron Or88a | somatic clone

(Stephan et al., 2012)

embryonic/larval hemocyte (with Df(3R)H15)

(Brennan et al., 2007)

lamellipodium

(Stephan et al., 2012)

melanotic mass

(Wu et al., 2001)

melanotic mass (with Df(3R)H15)

(Brennan et al., 2007)

Detailed Description

Statement

Reference

Homozygous olfactory receptor neuron (ORN) clones result in complete loss of the Or71a, Or88a, Or42a and Or46a-expressing ORNs and a mild reduction in cell number of the Or10a, Or47a, Or22a, Or47b, Or33c and Or59c ORNs.

Homozygous ORN clones result in defects in glomerulus targeting. Mutant Or47a-expressing ORNs frequently innervate a ventromedial glomerulus (in wild-type animals they target the dorsomedial DM3 glomerulus). Mutant Or59c ORNs show a strong mistargeting phenotype. Mutant Or92a ORNs show a mistargeting phenotype.

Wild-type Or59c, Or42a and Or47a ORNs project normally in a psidin¹ background.

Neurons in primary neuron cultures derived from mutant embryos have significantly smaller lamellipodia and significantly longer neurites compared to wild-type controls.

(Stephan et al., 2012)

psidin¹ mutant larvae are unable to induce Def production.

psidin¹/Df(3R)H15 mutants are equally deficient in the induction of Def whether the infection was with a Gram-negative or Gram-positive micro-organism.

psidin¹/Df(3R)H15 mutants die after a prolonged third instar larval instar and develop melanotic masses two days after the normal time of pupariation. At this late time point, defects in the blood cells are detected, including necrotic and multinucleate cells. However, none of these defects are apparent at the early wandering stage. psidin¹/Df(3R)H15 mutants melanise wound sites normally.

(Brennan et al., 2007)

Mutant larvae show normal melanisation at a wound site in response to infection. Mutant larvae have a high frequency of melanotic tumours (penetrance of this phenotype is approximately 80%).

(Wu et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

psidin¹ has abnormal neuroanatomy | adult stage | somatic clone phenotype, enhanceable by tsr^S3E.UAS/Scer\GAL4^Act.PU

(Stephan et al., 2012)

NOT Enhanced by

Statement

Reference

psidin¹ has abnormal neuroanatomy | adult stage | somatic clone phenotype, non-enhanceable by Scer\GAL4^Act.PU/Naa20A^KK102646

(Stephan et al., 2012)

Suppressed by

Statement

Reference

psidin¹ has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by Scer\GAL4^Act.PU/tsr^S3A.UAS

(Stephan et al., 2012)

psidin¹ has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by Scer\GAL4^Act.PU/LIMK1^JF02063

(Stephan et al., 2012)

psidin¹ has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by Scer\GAL4^Act.PU/LIMK1^{UAS.KI.Tag:HA}

(Stephan et al., 2012)

psidin¹ has abnormal neuroanatomy | somatic clone | adult stage phenotype, suppressible by Scer\GAL4^Act.PU/BacA\p35^UAS.cUa

(Stephan et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

psidin¹ has adult olfactory receptor neuron Or59c | somatic clone phenotype, enhanceable by tsr^S3E.UAS/Scer\GAL4^Act.PU

(Stephan et al., 2012)

NOT Enhanced by

Statement

Reference

psidin¹ has adult olfactory receptor neuron Or42a | somatic clone phenotype, non-enhanceable by Scer\GAL4^Act.PU/Naa20A^KK102646

(Stephan et al., 2012)

psidin¹ has adult olfactory receptor neuron Or59c | somatic clone phenotype, non-enhanceable by Scer\GAL4^Act.PU/Naa20A^KK102646

(Stephan et al., 2012)

Suppressed by

Statement

Reference

psidin¹ has adult olfactory receptor neuron Or59c | somatic clone phenotype, suppressible by Scer\GAL4^Act.PU/tsr^S3A.UAS

(Stephan et al., 2012)

psidin¹ has adult olfactory receptor neuron Or59c | somatic clone phenotype, suppressible by Scer\GAL4^Act.PU/LIMK1^JF02063

(Stephan et al., 2012)

psidin¹ has adult olfactory receptor neuron Or59c | somatic clone phenotype, suppressible by Scer\GAL4^Act.PU/LIMK1^{UAS.KI.Tag:HA}

(Stephan et al., 2012)

psidin¹ has adult olfactory receptor neuron Or59c | somatic clone phenotype, suppressible by Scer\GAL4^Act.PU/BacA\p35^UAS.cUa

(Stephan et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of tsr^S3A.Scer\UAS under the control of Scer\GAL4^Act.PU reduces the axon mistargeting phenotype seen in Or59c-expressing psidin¹ olfactory receptor neuron (ORN) clones by 14%.

Expression of tsr^S3E.Scer\UAS under the control of Scer\GAL4^Act.PU increases the axon mistargeting phenotype seen in Or59c-expressing psidin¹ ORN clones by 8%.

Or59c-expressing ORN clones which are mutant for psidin¹ and which are also expressing LIMK1^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Act.PU show mistargeting in 87% of cases.

Expression of either LIMK1^JF02063 or LIMK1^{Scer\UAS.KI.T:Ivir\HA1} under the control of Scer\GAL4^Act.PU significantly rescues the axon mistargeting phenotype seen in Or59c-expressing psidin¹ ORN clones.

Expression of NAA20^KK102646 under the control of Scer\GAL4^Act.PU does not enhance the reduction in cell number and axon mistargeting defects seen in Or42a and Or59c ORNs homozygous for psidin¹.

(Stephan et al., 2012)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^Scer\UAS.cUa under the control of Scer\GAL4^Act.PU rescues the reduction in cell number but not the axon mistargeting phenotypes seen in psidin¹ Or59c olfactory receptor neuron clones.

(Stephan et al., 2012)

Complementation and Rescue Data

Rescued by

psidin¹ is rescued by Scer\GAL4^Act.PU/psidin^UAS.Tag:HA

(Stephan et al., 2012)

psidin¹ is rescued by Scer\GAL4^Act.PU/psidin^{S678A.UAS.Tag:HA}

(Stephan et al., 2012)

Partially rescued by

psidin¹ is partially rescued by psidin^{IG978.UAS.Tag:HA}/Scer\GAL4^Act.PU

(Stephan et al., 2012)

psidin¹ is partially rescued by psidin^{S678D.UAS.Tag:HA}/Scer\GAL4^Act.PU

(Stephan et al., 2012)

Comments

Expression of psidin^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Act.PU rescues the reduction in cell number and axon mistargeting phenotypes seen in psidin¹ Or59c olfactory receptor neuron (ORN) clones.

Expression of psidin^{IG978.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Act.PU fully rescues the cell loss phenotype seen in Or59c ORN clones which are homozygous for psidin¹, but the axon targeting defects of these ORNs are only partially rescued.

Expression of psidin^{S678D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Act.PU fully rescues the axon targeting defects seen in Or59c ORN clones which are homozygous for psidin¹, but the cell loss phenotype of these ORNs is not rescued.

Expression of psidin^{S678D.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Act.PU rescues the axon targeting defects and cell loss phenotypes seen in Or59c ORN clones which are homozygous for psidin¹.

(Stephan et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a mutation that fails to induce expression of Ecol\lacZ^Dpt.PR normally in response to infection.

(Wu et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ird16

(Wu et al., 2001)

ird16¹

psidin¹

(Stephan et al., 2012, Brennan, 2007, Brennan et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (4)

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