FlyBase Allele Report: Dmel\vib[EP513]

General Information

Symbol

Dmel\vib^EP513

Species

D. melanogaster

Name

FlyBase ID

FBal0128967

Feature type

allele

Associated gene

Dmel\vib

Associated Insertion(s)

P{EP}vib^EP513

Carried in Construct

Also Known As

gio^EP513

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{EP}vib^EP513

Cytology

Description

Insertion within the first exon of the vib gene.

(Gatt and Glover, 2006)

P{EP}vib^EP513 is inserted into the first, untranslated exon of the vib gene.

(Giansanti et al., 2006)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:19,226,587..19,226,587 [-]

(Branco et al., 2008, Gatt and Glover, 2006, Giansanti et al., 2006, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Branco et al., 2008)

ameliorates olivopontocerebellar atrophy

modeled by Hsap\ATXN1^82Q.UAS

(Branco et al., 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

larval brain

(Giansanti et al., 2006)

spermatid

(Giansanti et al., 2007)

Detailed Description

Statement

Reference

No retinal phenotypes are seen when vib^EP513 is expressed under the control of Scer\GAL4^GMR.PU.

(Branco et al., 2008)

vib^EP513 spermatids are multinucleated.

(Giansanti et al., 2007)

vib^EP513 homozygous mutants are embryonic lethal. vib^EP513/Df(3R)Dl-BX12 mutants are lethal during early pharate adult stages.

(Gatt and Glover, 2006)

Larval brains of vib^EP513 mutants exhibit frequent polyploid cells but have normal frequences of anaphases, consistent with a defect in neuroblast cytokinesis.

Approximately 61.7% of vib^EP513 mutant telophases display abnormal localisation of vesicle-like structures at the cell equator.

(Giansanti et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

vib^EP513 has abnormal cytokinesis | male phenotype, non-enhanceable by Rab11^93Bi/Rab11^ETo11

(Giansanti et al., 2007)

NOT suppressed by

Statement

Reference

vib^EP513 has abnormal cytokinesis | male phenotype, non-suppressible by Rab11^93Bi/Rab11^ETo11

(Giansanti et al., 2007)

NOT Enhancer of

Statement

Reference

vib^EP513 is a non-enhancer of abnormal cytokinesis | male phenotype of fwd^Z0453/fwd³

(Giansanti et al., 2007)

NOT Suppressor of

Statement

Reference

vib^EP513 is a non-suppressor of abnormal cytokinesis | male phenotype of fwd^Z0453/fwd³

(Giansanti et al., 2007)

Other

Statement

Reference

Rab11^93Bi/Rab11^ETo11, vib^EP513 has abnormal cytokinesis | male phenotype

(Giansanti et al., 2007)

fwd^Z0453/fwd³, vib^EP513 has abnormal cytokinesis | male phenotype

(Giansanti et al., 2007)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

vib^EP513 has spermatid phenotype, non-enhanceable by Rab11^93Bi/Rab11^ETo11

(Giansanti et al., 2007)

NOT suppressed by

Statement

Reference

vib^EP513 has spermatid phenotype, non-suppressible by Rab11^93Bi/Rab11^ETo11

(Giansanti et al., 2007)

Enhancer of

Statement

Reference

vib^EP513, Scer\GAL4^GMR.PU is an enhancer of retina phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Branco et al., 2008)

NOT Enhancer of

Statement

Reference

vib^EP513 is a non-enhancer of spermatid phenotype of fwd^Z0453/fwd³

(Giansanti et al., 2007)

Suppressor of

Statement

Reference

vib^EP513, Scer\GAL4^GMR.PU is a suppressor of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Branco et al., 2008)

NOT Suppressor of

Statement

Reference

vib^EP513 is a non-suppressor of spermatid phenotype of fwd^Z0453/fwd³

(Giansanti et al., 2007)

Other

Statement

Reference

Rab11^93Bi/Rab11^ETo11, vib^EP513 has spermatid phenotype

(Giansanti et al., 2007)

fwd^Z0453/fwd³, vib^EP513 has spermatid phenotype

(Giansanti et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Rab11^93Bi/Rab11^ETo11, vib^EP513 spermatids are multinucleated at a similar frequency to vib^EP513 spermatids.

vib^EP513, fwd³/fwd^Z0453 spermatids are multinucleated at a similar frequency to fwd³/fwd^Z0453 spermatids.

(Giansanti et al., 2007)

Xenogenetic Interactions

Statement

Reference

Expression of vib^EP513 enhances the retinal phenotype seen when Hsap\HTT^{128Q.1-336.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PU.

Expression of vib^EP513 suppresses the retinal phenotype seen when Hsap\ATXN1^82Q.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PU.

Expression of vib^EP513 reduces the number of neurons that contain nuclear inclusions seen when Hsap\ATXN1^82Q.Scer\UAS is expressed under the control of Scer\GAL4^ey-OK107.

Expression of vib^EP651 increases the number of neurons that contain nuclear inclusions seen when Hsap\HTT^{128Q.1-336.Scer\UAS} is expressed under the control of Scer\GAL4^ey-OK107.

(Branco et al., 2008)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Separable from: A second site mutation causing early embryonic lethality.

(Gatt and Glover, 2006)

Comments

Based on the lethal stage of hemizygous alleles (over Df(3R)Dl-BX12), the following vib alleles can be ranked from strongest to weakest as follows: vib^j7A3 > vib^S110416 > vib^EP513 = vib^j5A6 > vib^S045002c > vib^EP651.

(Gatt and Glover, 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

gio^EP513

(Belloni et al., 2012, Giansanti et al., 2007, Giansanti et al., 2006)

vib^EP513

(Gatt and Glover, 2006)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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