FlyBase Allele Report: Dmel\fwd[3]

General Information

Symbol

Dmel\fwd³

Species

D. melanogaster

Name

FlyBase ID

FBal0095413

Feature type

allele

Associated gene

Dmel\fwd

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Brill et al., 2000)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q310term.

(Brill et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:309,810..309,810 [-]

Nucleotide change:

C309810T

Amino acid change:

Reported amino acid change:

Q310term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Brill et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis (with Df(3L)7C)

(Polevoy et al., 2009)

abnormal cytokinesis | male (with fwd^Z0453)

(Giansanti et al., 2007)

abnormal locomotor behavior | adult stage (with Df(3L)7C)

(Terriente-Felix et al., 2020)

abnormal meiotic cell cycle (with Df(3L)7C)

(Polevoy et al., 2009)

abnormal meiotic cell cycle | male (with Df(3L)7C)

(Brill et al., 2000)

male sterile (with Df(3L)7C)

(Polevoy et al., 2009)

short lived (with Df(3L)7C)

(Terriente-Felix et al., 2020)

viable (with Df(3L)7C)

(Brill et al., 2000)

Phenotype Manifest In

larval ventral nerve cord | larval stage (with Df(3L)7C)

(Terriente-Felix et al., 2020)

mitochondrion | larval stage (with Df(3L)7C)

(Terriente-Felix et al., 2020)

spermatid

(Giansanti et al., 2007)

spermatid (with Df(3L)7C)

(Polevoy et al., 2009, Giansanti et al., 2007)

spermatid (with fwd^Z0453)

(Giansanti et al., 2007)

spermatid & nucleus (with Df(3L)7C)

(Brill et al., 2000)

Detailed Description

Statement

Reference

fwd³/Df(3L)7C adults show decreased climbing activity. Mitochondria in the neuronal cell bodies from the larval ventral ganglion show increased length and connectivity (number of branches).

(Terriente-Felix et al., 2020)

The larval brain of fwd³/Df(3L)7C transheterozygotes does not exhibit significant changes in the number of neuroblasts, as compared to controls.

(Koe et al., 2018)

The glue granules in fwd³ mutant third instar larval instar salivary glands are a normal diameter compared to wild type.

(Burgess et al., 2012)

No significant differences are found in the amplitude of evoked EJPs or the frequency or amplitude of miniature EJPs in fwd³/Df(3L)7C mutants compared to controls. Nor are there differences in the number of quanta released by 2Hz stimulation, or in the amplitude or the frequency of spontaneously occurring quantal events of 1b boutons.

There are no significant differences in the number of 1b or 1s boutons in fwd³ mutants compared to controls.

(Dason et al., 2009)

fwd³/Df(3L)7C mutant testes exhibit multinucleate spermatids containing either two or four haploid nuclei accompanied by an enlarged mitochondrial derivative. There is a failure of meiotic cytokinesis. Males are infertile.

(Polevoy et al., 2009)

fwd³/fwd^Z0453 spermatids are multinucleated.

(Giansanti et al., 2007)

99.3% of spermatids in the testes of fwd³/Df(3L)7C males are defective, being derived from one or more defective meiotic and/or mitotic divisions. Spermatids with 2 or 4 nuclei per mitochondrial derivative are often seen, and spermatids with 3 or 8 nuclei per mitochondrial derivative are also sometimes seen.

(Brill et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

fwd^Z0453/fwd³ has abnormal cytokinesis | male phenotype, non-enhanceable by Rab11^93Bi/Rab11^ETo11

(Giansanti et al., 2007)

fwd^Z0453/fwd³ has abnormal cytokinesis | male phenotype, non-enhanceable by vib^EP513

(Giansanti et al., 2007)

Suppressed by

Statement

Reference

Df(3L)7C/fwd³ has abnormal meiotic cell cycle phenotype, suppressible by Rab11^{Q70L.βTub85D}

(Polevoy et al., 2009)

Df(3L)7C/fwd³ has abnormal cytokinesis phenotype, suppressible by Rab11^{Q70L.βTub85D}

(Polevoy et al., 2009)

Df(3L)7C/fwd³ has male sterile phenotype, suppressible by Btau\PI4KB^βTub85D.PP

(Polevoy et al., 2009)

Df(3L)7C/fwd³ has abnormal meiotic cell cycle phenotype, suppressible by Btau\PI4KB^βTub85D.PP

(Polevoy et al., 2009)

Df(3L)7C/fwd³ has abnormal cytokinesis phenotype, suppressible by Btau\PI4KB^βTub85D.PP

(Polevoy et al., 2009)

Df(3L)7C/fwd³ has male sterile phenotype, suppressible by Hsap\PI4KB^{βTub85D.Tag:HA}

(Polevoy et al., 2009)

Df(3L)7C/fwd³ has abnormal meiotic cell cycle phenotype, suppressible by Hsap\PI4KB^{βTub85D.Tag:HA}

(Polevoy et al., 2009)

Df(3L)7C/fwd³ has abnormal cytokinesis phenotype, suppressible by Hsap\PI4KB^{βTub85D.Tag:HA}

(Polevoy et al., 2009)

Df(3L)7C/fwd³ has abnormal meiotic cell cycle phenotype, suppressible | partially by Hsap\PI4KB^KD.βTub85D

(Polevoy et al., 2009)

Df(3L)7C/fwd³ has abnormal cytokinesis phenotype, suppressible | partially by Hsap\PI4KB^KD.βTub85D

(Polevoy et al., 2009)

NOT suppressed by

Statement

Reference

Df(3L)7C/fwd³ has abnormal cytokinesis phenotype, non-suppressible by Rab11^{S25N.βTub85D}

(Polevoy et al., 2009)

Df(3L)7C/fwd³ has male sterile phenotype, non-suppressible by Hsap\PI4KB^KD.βTub85D

(Polevoy et al., 2009)

Df(3L)7C/fwd³ has abnormal meiotic cell cycle phenotype, non-suppressible by Rab11^βTub85D.PP

(Polevoy et al., 2009)

Df(3L)7C/fwd³ has abnormal cytokinesis phenotype, non-suppressible by Rab11^βTub85D.PP

(Polevoy et al., 2009)

Df(3L)7C/fwd³ has abnormal meiotic cell cycle phenotype, non-suppressible by Rab11^{S25N.βTub85D}

(Polevoy et al., 2009)

fwd^Z0453/fwd³ has abnormal cytokinesis | male phenotype, non-suppressible by Rab11^93Bi/Rab11^ETo11

(Giansanti et al., 2007)

fwd^Z0453/fwd³ has abnormal cytokinesis | male phenotype, non-suppressible by vib^EP513

(Giansanti et al., 2007)

NOT Enhancer of

Statement

Reference

Df(3L)7C/fwd³, Scer\GAL4^elav-C155 is a non-enhancer of abnormal neurophysiology phenotype of Frq1^UAS.cRPa, Scer\GAL4^elav-C155

(Dason et al., 2009)

NOT Suppressor of

Statement

Reference

Df(3L)7C/fwd³, Scer\GAL4^elav-C155 is a non-suppressor of abnormal neurophysiology phenotype of Frq1^UAS.cRPa, Scer\GAL4^elav-C155

(Dason et al., 2009)

Other

Statement

Reference

Df(3L)7C/fwd³, sau^Z2217 has lethal - all die during larval stage | recessive phenotype

(Sechi et al., 2014)

sau^Z2217/Df(2L)Exel7010, Df(3L)7C/fwd³ has lethal - all die during larval stage phenotype

(Sechi et al., 2014)

Rab11^93Bi/Rab11^ETo11, fwd^Z0453/fwd³ has abnormal cytokinesis | male phenotype

(Giansanti et al., 2007)

fwd^Z0453/fwd³, vib^EP513 has abnormal cytokinesis | male phenotype

(Giansanti et al., 2007)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

fwd^Z0453/fwd³ has spermatid phenotype, non-enhanceable by Rab11^93Bi/Rab11^ETo11

(Giansanti et al., 2007)

fwd^Z0453/fwd³ has spermatid phenotype, non-enhanceable by vib^EP513

(Giansanti et al., 2007)

Suppressed by

Statement

Reference

Df(3L)7C/fwd³ has spermatid phenotype, suppressible by Rab11^{Q70L.βTub85D}

(Polevoy et al., 2009)

Df(3L)7C/fwd³ has spermatid phenotype, suppressible by Btau\PI4KB^βTub85D.PP

(Polevoy et al., 2009)

Df(3L)7C/fwd³ has spermatid phenotype, suppressible by Hsap\PI4KB^{βTub85D.Tag:HA}

(Polevoy et al., 2009)

Df(3L)7C/fwd³ has spermatid phenotype, suppressible | partially by Hsap\PI4KB^KD.βTub85D

(Polevoy et al., 2009)

NOT suppressed by

Statement

Reference

Df(3L)7C/fwd³ has spermatid phenotype, non-suppressible by Rab11^βTub85D.PP

(Polevoy et al., 2009)

Df(3L)7C/fwd³ has spermatid phenotype, non-suppressible by Rab11^{S25N.βTub85D}

(Polevoy et al., 2009)

fwd^Z0453/fwd³ has spermatid phenotype, non-suppressible by Rab11^93Bi/Rab11^ETo11

(Giansanti et al., 2007)

fwd^Z0453/fwd³ has spermatid phenotype, non-suppressible by vib^EP513

(Giansanti et al., 2007)

Other

Statement

Reference

Rab11^93Bi/Rab11^ETo11, fwd^Z0453/fwd³ has spermatid phenotype

(Giansanti et al., 2007)

fwd^Z0453/fwd³, vib^EP513 has spermatid phenotype

(Giansanti et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

sau^Z2217/+ ; fwd³/Df(3L)7C, sau^Z2217/sau^Z2217 ; fwd³/+ and sau^Z2217/Df(2L)Exel6007 ; fwd³/+ animals are viable, but sau^Z2217/Df(2L)Exel6007 ; fwd³/Df(3L)7C and sau^Z2217/sau^Z2217 ; fwd³/Df(3L)7C double mutants die during larval stages.

(Sechi et al., 2014)

The presence of a fwd³/Df(3L)7C background does not affect the enhanced quantal release found upon overexpression of Frq1^{Scer\UAS.cRPa} under the control of Scer\GAL4^elav-C155.

The presence of a fwd³/Df(3L)7C background does not affect the reduced number of synaptic boutons observed upon overexpression of Frq1^{Scer\UAS.cRPa} under the control of Scer\GAL4^elav-C155.

(Dason et al., 2009)

Overexpression of Rab11^{Q70L.βTub85D} but not Rab11^βTub85D.PP or Rab11^{S25N.βTub85D} partially suppresses the fwd³/Df(3L)7C cytokinesis defect.

(Polevoy et al., 2009)

Rab11^93Bi/Rab11^ETo11, fwd³/fwd^Z0453 spermatids are multinucleated at a similar frequency to fwd³/fwd^Z0453 spermatids.

vib^EP513, fwd³/fwd^Z0453 spermatids are multinucleated at a similar frequency to fwd³/fwd^Z0453 spermatids.

(Giansanti et al., 2007)

Xenogenetic Interactions

Statement

Reference

Expression of Btau\PI4KB^βTub85D.PP fully rescues the cytokinesis defects and infertility of fwd³/Df(3L)7C mutant males.

Expression of Hsap\PI4KB^{βTub85D.T:Ivir\HA1} fully rescues the cytokinesis defects and infertility of fwd³/Df(3L)7C mutant males.

Expression of Hsap\PI4KB^KD.βTub85D partially rescues the cytokinesis defects of fwd³/Df(3L)7C mutant males. Male infertility is not rescued in these animals.

(Polevoy et al., 2009)

Complementation and Rescue Data

Rescued by

Df(3L)7C/fwd³ is rescued by fwd^UAS.GFP/Scer\GAL4^da.G32

(Terriente-Felix et al., 2020)

fwd³ is rescued by Df(3L)7C/fwd^Hsp83.GFP

(Polevoy et al., 2009)

Partially rescued by

fwd³ is partially rescued by fwd^KD.Hsp83.GFP/Df(3L)7C

(Polevoy et al., 2009)

Comments

fwd^T:Avic\GFP completely rescues the cytokinesis defects and infertility of fwd³/Df(3L)7C mutant males.

fwd^{KD.T:Avic\GFP} partially rescues the cytokinesis defects of fwd³/Df(3L)7C mutant males. Male infertility is not rescued in these animals.

(Polevoy et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

fwd

(Burgess et al., 2012)

fwd³

(Terriente-Felix et al., 2020, Koe et al., 2018, Sechi et al., 2014, Goldbach et al., 2010, Dason et al., 2009, Polevoy et al., 2009, Giansanti et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (10)

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