FlyBase Allele Report: Dmel\Pex13[KG04339]

General Information

Symbol

Dmel\Pex13^KG04339

Species

D. melanogaster

Name

FlyBase ID

FBal0134334

Feature type

allele

Associated gene

Dmel\Pex13

Associated Insertion(s)

P{SUPor-P}Pex13^KG04339

Carried in Construct

Also Known As

KG04339

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{SUPor-P}Pex13^KG04339

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{SUPor-P}

insertional mutagenesis tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:13,230,075..13,230,075 [+]

(Chen et al., 2010, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of peroxisomal disease

CEA

(Chen et al., 2010)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis

(Chen et al., 2010)

abnormal meiotic cell cycle

(Chen et al., 2010)

viable

(Chen et al., 2010)

Phenotype Manifest In

spermatid

(Chen et al., 2010)

testis

(Chen et al., 2010)

Detailed Description

Statement

Reference

In squashed testis preparations, spermatocytes as well as elongated spermatid bundles are present n young pex13^KG04339 mutants. At the onion stage, most round spermatids are abnormal. Spermatids usually have four nuclei and one large mitochondrial derivative, indicative of a cytokinesis defect. This phenotype progressively worsened with age. In older mutant animals, 'cotton ball'-like spheres are also present.

Growing pex13^KG04339 mutants on medium to which 2.5% VLCFA has been added results in a significant enhancement of the pex10⁷ testis phenotype compared to pex10⁷ flies grown on non-supplemented food. The elongated spermatid bundles that are usually present under normal food conditions disappear, replaced with 'cotton ball'-like structures. In contrast, adding 2.5% LCFA to the food does not enhance the testis phenotype.

(Chen et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Pex13^KG04339 has abnormal cytokinesis phenotype, enhanceable by Scer\GAL4^nanos.PG/Tecr^EY02236

(Chen et al., 2010)

Suppressed by

Statement

Reference

Pex13^KG04339 has abnormal cytokinesis phenotype, suppressible by Tecr^EY02236/Tecr⁰⁵⁶³⁴

(Chen et al., 2010)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

pex13[+]/Pex13^KG04339 is a non-enhancer of wing margin phenotype of Bx²

(Bronstein et al., 2010)

NOT Suppressor of

Statement

Reference

pex13[+]/Pex13^KG04339 is a non-suppressor of wing margin phenotype of Bx²

(Bronstein et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Overexpression of Sc2^EY02236 under the control of Scer\GAL4^nos.PG enhances the pex13^KG04339 cytokinesis defect.

Sc2⁰⁵⁶³⁴/Sc2^EY02236 transheterozygotes partially suppress the pex13^KG04339 cytokinesis defect.

(Chen et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

13804

y¹ w^67c23; P{SUPor-P}Pex13^KG04339

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG4663^KG04339

KG04339

(Bronstein et al., 2010)

Pex13^KG04339

pex13^KG04339

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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