FlyBase Allele Report: Dmel\rg[γ5]

General Information

Symbol

Dmel\rg^γ5

Species

D. melanogaster

Name

FlyBase ID

FBal0138152

Feature type

allele

Associated gene

Dmel\rg

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

hypomorphic allele - molecular evidence

Mutagen

gamma ray

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Shamloula et al., 2002)

hypomorphic allele - molecular evidence

(Volders et al., 2012)

Mutagen

gamma ray

(Shamloula et al., 2002)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult mushroom body

(Volders et al., 2012)

eye

(Shamloula et al., 2002)

eye (with Df(1)JC70)

(Shamloula et al., 2002)

eye (with rg¹)

(Shamloula et al., 2002)

type I bouton

(Wise et al., 2015)

Detailed Description

Statement

Reference

rg^γ5 mutants display abnormal type I bouton morphology: changes in shape, fusion of boutons. Size of type I boutons remains unchanged but their number is significantly decreased.

rg^γ5 mutants show decreased frequency of peristaltic contractions, decreased speed of movement and are more likely to be in a curled position.

rg^γ5 mutants show increased habituation of the giant fiber circuit in adults.

(Wise et al., 2015)

Mutant flies show a significant reduction in aversive and appetitive memory compared to controls when assayed immediately after olfactory conditioning.

43% of mushroom body hemispheres have morphological defects in hemizygous males.

(Volders et al., 2012)

Moderate rough eye phenotype.

(Shamloula et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

rg^γ5 has abnormal learning phenotype, suppressible | partially by Scer\GAL4^elav.PU/Mmus\Nbea^UAS.cVa

(Volders et al., 2012)

NOT suppressed by

Statement

Reference

rg^γ5 has abnormal neuroanatomy | adult stage phenotype, non-suppressible by Scer\GAL4^elav.PU/Mmus\Nbea^UAS.cVa

(Volders et al., 2012)

rg^γ5 has abnormal neuroanatomy | adult stage phenotype, non-suppressible by Scer\GAL4^ey-OK107/Mmus\Nbea^UAS.cVa

(Volders et al., 2012)

rg^γ5 has abnormal neuroanatomy | adult stage phenotype, non-suppressible by Mmus\Nbea^UAS.cVa/Scer\GAL4^Mef2.247

(Volders et al., 2012)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

rg^γ5 has adult mushroom body phenotype, non-suppressible by Scer\GAL4^elav.PU/Mmus\Nbea^UAS.cVa

(Volders et al., 2012)

rg^γ5 has adult mushroom body phenotype, non-suppressible by Scer\GAL4^ey-OK107/Mmus\Nbea^UAS.cVa

(Volders et al., 2012)

rg^γ5 has adult mushroom body phenotype, non-suppressible by Mmus\Nbea^UAS.cVa/Scer\GAL4^Mef2.247

(Volders et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Expression of Mmus\Nbea^Scer\UAS.cVa under the control of Scer\GAL4^elav.PU completely rescues the loss of immediate aversive memory seen in rg^γ5 flies but fails to rescue the loss of immediate appetitive memory seen in rg^γ5 flies in an olfactory conditioning assay.

The mushroom body defects seen in hemizygous rg^γ5 males are not rescued by expression of Mmus\Nbea^Scer\UAS.cVa under the control of either Scer\GAL4^elav.PU, Scer\GAL4^ey-OK107 or Scer\GAL4^Mef2.247.

(Volders et al., 2012)

Complementation and Rescue Data

Fails to complement

rg¹

(Shamloula et al., 2002)

Rescued by

rg^γ5 is rescued by Scer\GAL4^elav.PU/rg^UAS.cVa

(Volders et al., 2012)

rg^γ5 is rescued by Scer\GAL4^ey-OK107/rg^UAS.cVa

(Volders et al., 2012)

Partially rescued by

rg^γ5 is partially rescued by Scer\GAL4^Mef2.247/rg^UAS.cVa

(Volders et al., 2012)

Not rescued by

rg^γ5 is not rescued by Scer\GAL4^GH298/rg^UAS.cVa

(Volders et al., 2012)

rg^γ5 is not rescued by rg^UAS.cVa/Scer\GAL4^GH146

(Volders et al., 2012)

Comments

Expression of rg^Scer\UAS.cVa under the control of Scer\GAL4^elav.PU completely rescues the loss of immediate aversive and appetitive memory seen in rg^γ5 flies in an olfactory conditioning assay. If expression of rg^Scer\UAS.cVa is restricted to the adult stage 36 hours before the olfactory conditioning assay (using the temperature-sensitive Scer\GAL80^ts.αTub84B allele), the rescue of aversive memory is abolished.

Expression of rg^Scer\UAS.cVa under the control of either Scer\GAL4^Mef2.247 or Scer\GAL4^ey-OK107 rescues the loss of immediate aversive and appetitive memory seen in rg^γ5 flies in an olfactory conditioning assay.

The mushroom body defects seen in hemizygous rg^γ5 males are rescued by expression of rg^Scer\UAS.cVa under the control of either Scer\GAL4^elav.PU or Scer\GAL4^ey-OK107, but are not rescued by expression under the control of Scer\GAL4^Mef2.247.

Expression of rg^Scer\UAS.cVa under the control of either Scer\GAL4^GH298 or Scer\GAL4^GH146 fails to rescue the loss of immediate aversive and appetitive memory seen in rg^γ5 flies in an olfactory conditioning assay.

(Volders et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

78792

w^* rg^γ5

78794

w^* rg^γ5; P{UAS-Nbea.V}2

78793

w^* rg^γ5; P{UAS-rg.V}2/CyO