FlyBase Allele Report: Dmel\SoxN[UAS.cOa]

General Information

Symbol

Dmel\SoxN^UAS.cOa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Overton

FlyBase ID

FBal0141378

Feature type

allele

Associated gene

Dmel\SoxN

Associated Insertion(s)

Carried in Construct

P{UAS-SoxN.O}

Also Known As

UAS-SoxN

Key Links

Transgenic product class

wild_type

(Overton et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Overton et al., 2002)

Mutagen

in vitro construct

(Overton et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-SoxN.O}

Cytology

Description

UASt regulatory sequences drive expression of the entire SoxN open reading frame.

(Overton et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

SoxN

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage, with Scer\GAL4^e22c

(Chao et al., 2007)

partially lethal - majority die, with Scer\GAL4^arm.PS

(Chao et al., 2007)

partially lethal - majority die, with Scer\GAL4^dpp.blk1

(Chao et al., 2007)

some die during embryonic stage, with Scer\GAL4^arm.PS

(Chao et al., 2007)

some die during embryonic stage, with Scer\GAL4^e22c

(Chao et al., 2007)

some die during pupal stage, with Scer\GAL4^dpp.blk1

(Chao et al., 2007)

Phenotype Manifest In

denticle | ectopic, with Scer\GAL4^en-e16E

(Overton et al., 2007)

denticle row | ectopic, with Scer\GAL4^e22c

(Chao et al., 2007)

embryonic/first instar larval cuticle, with Df(3R)Espl22, Scer\GAL4^e22c

(Chao et al., 2007)

embryonic/first instar larval cuticle, with Scer\GAL4^e22c

(Chao et al., 2007)

ventral denticle belt, with Df(3R)Espl22, Scer\GAL4^e22c

(Chao et al., 2007)

ventral denticle belt, with Scer\GAL4^e22c

(Chao et al., 2007)

Detailed Description

Statement

Reference

Overexpression of SoxN^Scer\UAS.cOa under the control of Scer\GAL4^e22c in an otherwise wild-type embryo affects wg-mediated cuticle patterning. Ectopic denticles replace some of the ventral and ventrolateral naked cuticles, and dorsal patterning is disrupted, leading to curvature of the embryo. An average of 12 ectopic denticles are found within the naked cuticle zone of a typical abdominal segment.

Expression of SoxN^Scer\UAS.cOa under the control of Scer\GAL4^arm.PS results in approximately 32.9% embryonic lethality, while expression under the more potent Scer\GAL4^e22c driver results in approximately 77.7% embryonic lethality. In both cases, those embryos that hatch often survive to become normal adults, suggesting that SoxN plays no further role beyond embryogenesis. Expression under the control of Scer\GAL4^dpp.blk1 results in approximately 29.4% pupal lethality.

Overexpression of SoxN^Scer\UAS.cOa under the control of Scer\GAL4^e22c in embryos derived from mothers that were heterozygous for Df(3R)Espl22, produces milder pattern disruptions both dorsally and ventrally.

(Chao et al., 2007)

Expression of SoxN^Scer\UAS.cOa under the control of Scer\GAL4^en-e16E results in ectopic denticle formation in the larval cuticle.

(Overton et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

SoxN^UAS.cOa has lethal | embryonic stage phenotype, enhanceable by Scer\GAL4^arm.PS/pan^UAS.cWa

(Chao et al., 2007)

SoxN^UAS.cOa has lethal | embryonic stage phenotype, enhanceable by pan^UAS.cWa/Scer\GAL4^e22c

(Chao et al., 2007)

SoxN^UAS.cOa has lethal | pupal stage phenotype, enhanceable by Scer\GAL4^dpp.blk1/pan^UAS.cWa

(Chao et al., 2007)

Enhancer of

Statement

Reference

Scer\GAL4^arm.PS/SoxN^UAS.cOa is an enhancer of lethal | embryonic stage phenotype of pan^UAS.cWa

(Chao et al., 2007)

SoxN^UAS.cOa/Scer\GAL4^e22c is an enhancer of lethal | embryonic stage phenotype of pan^UAS.cWa

(Chao et al., 2007)

Scer\GAL4^dpp.blk1/SoxN^UAS.cOa is an enhancer of lethal | pupal stage phenotype of pan^UAS.cWa

(Chao et al., 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

SoxN^UAS.cOa has embryonic/first instar larval cuticle phenotype, enhanceable by Scer\GAL4^arm.PS/pan^UAS.cWa

(Chao et al., 2007)

SoxN^UAS.cOa has ventral denticle belt phenotype, enhanceable by Scer\GAL4^arm.PS/pan^UAS.cWa

(Chao et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of both SoxN^Scer\UAS.cOa and pan^Scer\UAS.cWa under the control of Scer\GAL4^arm.PS results in 87.6% embryonic lethality. Expression under the control of Scer\GAL4^e22c results in approximately 99.6% embryonic lethality, while expression of both SoxN^Scer\UAS.cOa and pan^Scer\UAS.cWa under the control of Scer\GAL4^dpp.blk1 results in approximately 100% pupal lethality.

Overexpression of pan^Scer\UAS.cWa enhances the repressive capacity of overexpressed SoxN^Scer\UAS.cOa. This is particularly apparent when both transgenes are expressed under the control of Scer\GAL4^arm.PS. Under these conditions, the cuticle pattern is most disrupted, both ventrally and dorsally. Ectopic denticles appear with greater frequency and broader distribution across the ventral naked cuticle domain.

(Chao et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

SoxN^UAS.cOa rescues SoxN^NC14

(Chao et al., 2007)

Partially rescues

Scer\GAL4^Kr.PM/SoxN^UAS.cOa partially rescues SoxN^U6-35

(Overton et al., 2002)

Comments

Expression of SoxN^Scer\UAS.cOa under the control of Scer\GAL4^arm.PS rescues the excessive naked-cuticle phenotype of SoxN^NC14 homozygotes. This treatment does not rescue embryonic lethality.

(Chao et al., 2007)

Expression of SoxN^Scer\UAS.cOa under the control of Scer\GAL4^Kr.PM in SoxN^U6-35 mutant embryos substantially rescues formation of RP2 neurons and eve lateral cluster (ELC) cells.

(Overton et al., 2002)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9937

w^*; Kr^If-1/CyO; P{UAS-SoxN.O}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

SoxN^Scer\UAS.cOa

SoxN^UAS.cOa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Overton

(Overton et al., 2002)

Secondary FlyBase IDs

References (9)

Report Sections

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