FlyBase Allele Report: Dmel\Rheb[AV4]

General Information

Symbol

Dmel\Rheb^AV4

Species

D. melanogaster

Name

FlyBase ID

FBal0141561

Feature type

allele

Associated gene

Dmel\Rheb

Associated Insertion(s)

P{Mae-UAS.6.11}Rheb^AV4

Carried in Construct

Also Known As

UAS-Rheb^AV4

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Patel et al., 2003)

Mutagen

P-element activity

(Patel et al., 2003)

Progenitor genotype

Associated Insertion(s)

P{Mae-UAS.6.11}Rheb^AV4

Cytology

Description

P{Mae-UAS.6.11} insertion within the 5' UTR of Rheb at position +85 relative to the transcription start site. The insertion is in the right orientation to drive overexpression of Rheb in the presence of a Scer\GAL4 driver.

(Patel et al., 2003)

Allele components

Component

Use(s)

Inserted element

P{Mae-UAS.6.11}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell size | somatic clone

(Patel et al., 2003)

abnormal developmental rate

(Patel et al., 2003)

abnormal eclosion rhythm | dominant | drug conditional

(Patel et al., 2003)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^BG380

(Natarajan et al., 2013)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav.PLu

(Natarajan et al., 2013)

decreased body size | first instar larval stage | recessive

(Patel et al., 2003)

increased size | larval stage, with Hsap\RELA^{p65(AD)::pMag.Ubi}, Scer\GAL4^{rn-G4DBD::nMagHigh1}

(El Marzkioui et al., 2025)

lethal, with Scer\GAL4^byn-Gal4

(Patel et al., 2003)

lethal - all die before end of larval stage | recessive

(Patel et al., 2003)

lethal - all die before end of second instar larval stage | recessive

(Patel et al., 2003)

some die during larval stage | recessive

(Patel et al., 2003)

some die during second instar larval stage | recessive

(Patel et al., 2003)

Phenotype Manifest In

antenna, with Scer\GAL4^Act5C.PI

(Patel et al., 2003)

embryonic/larval hindgut, with Scer\GAL4^byn-Gal4

(Patel et al., 2003)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^BG380

(Natarajan et al., 2013)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^elav.PLu

(Natarajan et al., 2013)

eye, with Scer\GAL4^Act5C.PI

(Patel et al., 2003)

eye, with Scer\GAL4^GMR.PF

(Goberdhan et al., 2005, Patel et al., 2003)

eye | somatic clone

(Patel et al., 2003)

head, with Scer\GAL4^Act5C.PI

(Patel et al., 2003)

head | somatic clone

(Patel et al., 2003)

interommatidial bristle | increased number, with Scer\GAL4^Act5C.PI

(Patel et al., 2003)

ommatidium, with Scer\GAL4^Act5C.PI

(Patel et al., 2003)

ommatidium | somatic clone

(Patel et al., 2003)

wing disc | larval stage, with Hsap\RELA^{p65(AD)::pMag.Ubi}, Scer\GAL4^{rn-G4DBD::nMagHigh1}

(El Marzkioui et al., 2025)

Detailed Description

Statement

Reference

The neuromuscular junctions of larvae (muscle 4 of segment A2-4) expressing Rheb^AV4 under the control of either Scer\GAL4^elav.PLu or Scer\GAL4^BG380 show a significant increase in synaptic growth compared to controls. Moreover, unlike in wild type, "small bouton-like" extensions are often seen emanating from the interbouton area of the NMJs.

(Natarajan et al., 2013)

Expression of Rheb^AV4, under the control of Scer\GAL4^GMR.PF, produces a greatly enlarged eye.

(Goberdhan et al., 2005)

Lethality acts in the second larval instar. Generation of clones of cells in the eye disc that overexpress DRheb results in enlarged eyes and head, while generation of loss-of-function clones in a sensitized (Minute) background results in a dramatic reduction of eye and head size.

(Patel et al., 2003)

Expression of Rheb^AV4 driven by Scer\GAL4^byn-Gal4 causes lethality. This expression also causes a dramatically enlarged (both in width and length) hindgut in the first instar larva. Expression of Rheb^AV4 driven by Scer\GAL4^GMR.PF results in marked overgrowth of the eye compared with wild type. Expression of Rheb^AV4 driven by Scer\GAL4^Act5C.PI results in an enlarged eye, antenna and head. When compared to wild-type eyes, the ommatidia are much larger, are not organized into the normal hexagonal array and are frequently flanked by extra bristles. These bristles are thicker than in wild type. Embryos homozygous for Rheb^AV4 hatch into first instar larvae that grow very slowly, move lethargically, and die by 72 hours without molting into second instar larvae. Heads and eyes containing multiple Rheb^AV4 homozygous clones are dramatically reduced in size compared to those containing wild-type clones. Sections of eyes with Rheb^AV4 homozygous clones show disorganized, smaller ommatidia composed of smaller cells. Heterozygous Rheb^AV4 larvae usually emerge from the pupal case at the same time as in wild type. In the presence of rapamycin, however, Rheb^AV4 heterozygotes emerge two days later than their wild-type siblings.

(Patel et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Rheb[+]/Rheb^AV4 is a suppressor | partially of flightless | progressive phenotype of Pten¹/Pten⁵

(Mensah et al., 2015)

Rheb^AV4, Scer\GAL4^Act5C.PP is a suppressor | partially of increased cell death | somatic clone phenotype of Atg1^UAS.cSa, Scer\GAL4^Act5C.PP

(Scott et al., 2007)

Other

Statement

Reference

Rheb^AV4, Scer\GAL4^GMR.PF, foxo^GS9928 has lethal | male | pupal stage phenotype

(Goberdhan et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

Hsap\MAPT^V337M.UAS, Rheb^AV4, Scer\GAL4^GMR.PF has eye phenotype, suppressible by Cdk4³/Cdk4[+]

(Khurana et al., 2006)

Hsap\MAPT^V337M.UAS, Rheb^AV4, Scer\GAL4^GMR.PF has eye phenotype, suppressible by dap^UAS.cdNa/Rbf^UAS.cDa, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

Enhancer of

Statement

Reference

Rheb^AV4, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of Hsap\MAPT^V337M.UAS, Scer\GAL4^GMR.PF

(Khurana et al., 2006)

Rheb^AV4 is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, foxo^GS9928

(Goberdhan et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The elimination of cells expressing Atg1^Scer\UAS.cSa under the control of Scer\GAL4^Act5C.PP from the wing disc is delayed by co-expression of Rheb^AV4.

(Scott et al., 2007)

Co-expression of Rheb^AV4 results in a strong enhancement of Scer\GAL4^GMR.PF>foxo^GS9928, leading to loss of virtually all ommatidia in females and pupal lethality in males with massive tissue degeneration in the head of late pupae. These phenotypes are partially suppressed by Akt1^Scer\UAS.cSa overexpression.

(Goberdhan et al., 2005)

Xenogenetic Interactions

Statement

Reference

The rough-eye phenotype observed in animals expressing Hsap\MAPT^{V337M.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced by co-expression of Rheb^AV4. This enhancement is blocked in a Cdk4³/+ background, or by co-expressing dap^{Scer\UAS.cdNa} and Rbf^Scer\UAS.cDa.

The rough-eye phenotype observed in animals expressing Hsap\MJD^{tr.Q78.Scer\UAS.T:Ivir\HA1} in the eye under the control of Scer\GAL4^GMR.PF is not modified by co-expression of Rheb^AV4.

(Khurana et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9690

y¹ w^*; P{Mae-UAS.6.11}Rheb^AV4/TM6B, Tb¹

Notes on Origin

Discoverer

Comments

When the P{Mae-UAS.6.11} is excised the resulting chromosome combined with Scer\GAL4^byn-Gal4 shows no effect on hindgut size.

(Patel et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

DRheb^AV4

(Patel et al., 2003)

Rheb^AV4

(Blanco et al., 2020, Tang et al., 2018, Kim et al., 2017, Mensah et al., 2015, Zitserman et al., 2012)

dRheb^AV4

(Patel et al., 2003)

rheb^AV4

(Parker and Struhl, 2015)

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

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