FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Prosβ21.UAS
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General Information
Symbol
Dmel\Prosβ21.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0141583
Feature type
allele
Associated gene
Dmel\Prosβ2
Associated Insertion(s)
Carried in Construct
P{UAS-Prosβ21}
Also Known As
UAS-DTS7, UAS-Prosβ21, UAS-Prosβ2
Key Links
Transgenic product class
missense_variant
(Belote and Fortier, 2002)
Mutagen
Nature of the Allele
Transgenic product class
missense_variant
(Belote and Fortier, 2002)
Mutagen
in vitro construct
(Belote and Fortier, 2002)
Progenitor genotype
Prosβ21
(Belote and Fortier, 2002)
Carried in construct
P{UAS-Prosβ21}
Cytology
Description

UASt regulatory sequences drive expression of Prosβ21.

(Belote and Fortier, 2002)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Prosβ2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.long leads to rough eye phenotype due to ommatidial degeneration in adult flies maintained at 28[o]C, the phenotype is more severe when two copies of the transgene are used.

      (Oh et al., 2015)

      Expression of temperature-sensitive Prosβ2 under the control of Scer\GAL4GMR.PF results in rough eye phenotype at the non-permissive temperature of 28[o]C, at the permissive temperature (22[o]C) the eyes appear normal.

      (Rui et al., 2015)

      Co-expression of Prosβ21.Scer\UAS and Prosβ61.B.Scer\UAS under the control of Scer\GAL4wor.PA does not induce larval neuroblast loss.

      (Kuang et al., 2014)

      Expression of Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PF has no effect on eye pigmentation.

      (Estes et al., 2011)

      Flies expressing Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PF have normal eye morphology at 22oC, but at 28oC, substantial retinal degeneration is seen, resulting in a rough eye phenotype.

      Photoreceptor neurons show a significant increase in morphological features of autophagy in flies expressing Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PF at 28oC; there is an increase in the number of autophagic vacuoles and in the number of multilamellar bodies/multivesicular bodies.

      (Pandey et al., 2007)

      Expression of Prosβ21.Scer\UAS at 29oC under the control of Scer\GAL4GMR.PF results in severely disrupted eyes, with fused facets and small spots of black, presumably necrotic, cells.

      (Belote and Fortier, 2002)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      NOT suppressed by
      Statement
      Reference

      Prosβ21.UAS, Scer\GAL4GMR.PU has visible | heat sensitive phenotype, non-suppressible by Hsap\HDAC6H216A.H611A.UAS, Scer\GAL4GMR.PU

      (Pandey et al., 2007)
      Enhancer of
      Other
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      NOT suppressed by
      Enhancer of
      NOT Enhancer of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Flies co-expressing Prosβ61.B.Scer\UAS and Prosβ21.Scer\UAS under the control of Scer\GAL4elav.PU at the non-permissive temperature 30[o]C are lethal at the third instar larval stage. When the flies are raised at 18[o]C and then incubated at 30[o]C for 4 weeks and exposed to the pesticides paraquat and/or maneb no dopaminergic neuron loss is observed.

      (Martin et al., 2014)

      Co-expression of Prosβ61.B.Scer\UAS and Prosβ21.Scer\UAS in the salivary gland under the control of Scer\GAL4fkh.PH causes aberrant morphology of some salivary gland nuclei.

      (Zhang et al., 2014)

      Co-expression of Prosβ61.B.Scer\UAS and Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PU at 29[o]C results in a rough eye phenotype. This phenotype is enhanced if the animals are also co-expressing either TnksGD11407 or CG9588HM05013.

      Co-expression of Prosβ61.B.Scer\UAS and Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PU at 29[o]C results in a rough eye phenotype. This phenotype is enhanced in females if they are also co-expressing CG9588GD14259.

      Co-expression of Prosβ61.B.Scer\UAS, Prosβ21.Scer\UAS and TnksKK100530 under the control of Scer\GAL4GMR.PU at 29[o]C results in lethality.

      Co-expression of Prosβ61.B.Scer\UAS, Prosβ21.Scer\UAS and CG9588GD14259 under the control of Scer\GAL4GMR.PU at 29[o]C results in male lethality.

      (Cho-Park and Steller, 2013)

      Expression of both Pros261.B.Scer\UAS and Prosβ21.Scer\UAS using Scer\GAL4GMR.PU at 29[o]C causes a rough eye phenotype.

      Co-expression of PI31Scer\UAS.T:Ivir\HA1 suppresses the rough eye seen in flies expressing Pros261.B.Scer\UAS and Prosβ21.Scer\UAS using Scer\GAL4GMR.PU at 29[o]C.

      Whereas Scer\GAL4GMR.PU-mediated expression of either Prosβ21.Scer\UAS or PI31GD13764 alone gives no detectable eye phenotype at 25[o]C, their co-expression causes a strong eye phenotype. At 29[o]C, this combination is lethal.

      (Bader et al., 2011)

      Flies co-expressing Pros261.B.Scer\UAS and Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PF at 25[o]C appear near normal. Additional co-expression of P{Sym-UAS-Hsrω} does not alter the external eye morphology. However, the pseudopupil images of flies expressing Pros261.B.Scer\UAS and Prosβ21.Scer\UAS reveal severe retinal damage, which is substantially rescued by additional co-expression of one copy of P{Sym-UAS-Hsrω}.

      (Mallik and Lakhotia, 2010)

      Expression of Pros261.B.Scer\UAS and Prosβ21.Scer\UAS in the developing tracheal system under the control of Scer\GAL4btl.PS is sufficient to cause dorsal trunk breaks due to down-regulation of trh in embryonic fusion cells.

      (Mortimer and Moberg, 2007)

      The retinal degeneration seen in flies expressing Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PF at 28oC is suppressed by co-expression of HDAC6Scer\UAS.T:SV5\V5.

      The rough eye phenotype seen in flies expressing Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PF at 28oC is enhanced by co-expression of Atg6dsRNA.Scer\UAS or Atg12dsRNA.Scer\UAS.

      The ability of HDAC6Scer\UAS.T:SV5\V5 to suppress the rough eye phenotype seen in flies expressing Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PF at 28oC is suppressed by co-expression of Atg12dsRNA.Scer\UAS.

      (Pandey et al., 2007)
      Xenogenetic Interactions
      Statement
      Reference

      The eye degeneration phenotype observed in adult flies expressing either Zzzz\CGG90.Scer\UAS.T:Avic\GFP-EGFP or Zzzz\CGG100.5'UTR.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4GMR.long (and maintained at 28[o]C) is significantly enhanced by co-expression of Prosβ21.Scer\UAS (which on its own also induces rough eye phenotype), the eye degeneration is most severe when two copies of the Prosβ21.Scer\UAS transgene are used. Similarly, co-expression of Prosβ21.Scer\UAS also enhances the formation of inclusions in the ommatidia of Zzzz\CGG100.5'UTR.Scer\UAS.T:Avic\GFP-expressing flies.

      Co-expression of Prosβ21.Scer\UAS with either Zzzz\CGG100.5'Stop.Scer\UAS.T:Avic\GFP or Zzzz\CGG100.5'ATG.Scer\UAS.T:Avic\GFP also leads to mutual enhancement of the rough eye phenotype (of varied severity) each of the transgene produces on its own - combination with Zzzz\CGG100.5'Stop.Scer\UAS.T:Avic\GFP produces rather weak while combination with Zzzz\CGG100.5'ATG.Scer\UAS.T:Avic\GFP very strong phenotype enhancement (all ommatidia fused along with necrotic lesions).

      (Oh et al., 2015)

      Co-expression of the temperature sensitive Prosβ21.Scer\UAS allele together with Hsap\MAPTScer\UAS.T:Avic\GFP under the control of Scer\GAL4shot-OK307 has no adverse effect on the morphology and structure of the adult thorax or the thoracic somatic muscles below at either the non-permissive (28[o]C) or the permissive (22[o]C) temperatures.

      (Rui et al., 2015)

      Simultaneous co-expression of Prosβ61.B.Scer\UAS and Prosβ21.Scer\UAS does not suppress the protective role of expression of Hsap\ATXN3Scer\UAS.WT.T:Zzzz\His6 in suppression of eye degeneration in Scer\GAL4GMR.long>Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 flies. Simultaneous co-expression of Prosβ61.B.Scer\UAS and Prosβ21.Scer\UAS leads to lethality in Scer\GAL4GMR.long>Hsap\ATXN3tr.Q78.Scer\UAS.T:Ivir\HA1 flies; extra co-expression of Hsap\ATXN3Scer\UAS.WT.T:Zzzz\His6 suppresses this lethality.

      (Tsou et al., 2015)

      Expression of Prosβ21.Scer\UAS enhances the eye pigmentation phenotype seen when Hsap\TARDBPScer\UAS.T:Avic\GFP-YFP.cEa is expressed under the control of Scer\GAL4GMR.PF. This enhancement is seen throughout adult life at 25[o]C.

      Expression of Prosβ21.Scer\UAS enhances the eye pigmentation phenotype seen when Hsap\TARDBPA315T.Scer\UAS.T:Avic\GFP-YFP is expressed under the control of Scer\GAL4GMR.PF. This enhancement is seen throughout adult life at 25[o]C.

      (Estes et al., 2011)

      The eye damage caused by Scer\GAL4GMR.PF-mediated expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is enhanced by co-expression of Pros261.B.Scer\UAS and Prosβ21.Scer\UAS. Co-expression of P{Sym-UAS-Hsrω} still suppresses the damage in Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Pros261.B.Scer\UAS, Prosβ21.Scer\UAS eyes, but the eyes are not rescued to the degree seen in Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 eyes.

      The increase in inclusion bodies in larval eye discs caused by Scer\GAL4GMR.PF-mediated expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 is further increased by co-expression of Pros261.B.Scer\UAS and Prosβ21.Scer\UAS. However, co-expression of P{Sym-UAS-Hsrω} fails to reduce the number of inclusion bodies in the Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Pros261.B.Scer\UAS, Prosβ21.Scer\UAS background.

      The damaged eye phenotype that results from Scer\GAL4GMR.PF-mediated expression of Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 is not further enhanced by co-expression of Pros261.B.Scer\UAS and Prosβ21.Scer\UAS. Co-expression of P{Sym-UAS-Hsrω} still suppresses the damage in Scer\GAL4GMR.PF, Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1, Pros261.B.Scer\UAS, Prosβ21.Scer\UAS eyes, but the eyes are not rescued to the degree seen in Scer\GAL4GMR.PF, Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 eyes.

      (Mallik and Lakhotia, 2010)

      The retinal degeneration seen in flies expressing Prosβ21.Scer\UAS under the control of Scer\GAL4GMR.PF at 28oC is suppressed by co-expression of Hsap\HDAC6Scer\UAS.cPa, but not by co-expression of Hsap\HDAC6H216A.H611A.Scer\UAS.

      (Pandey et al., 2007)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      PSMB7-OE
      (Kim et al., 2021)
      Prosβ21.Scer\UAS
      Prosβ21.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (35)