FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Allele: Dmel\XpdSH2137
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General Information
Symbol
Dmel\XpdSH2137
Species
D. melanogaster
Name
FlyBase ID
FBal0143484
Feature type
allele
Associated gene
Dmel\Xpd
Associated Insertion(s)
P{lacW}XpdSH2137
Carried in Construct
Also Known As
xpdP
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Oh et al., 2003)
Progenitor genotype
Associated Insertion(s)
P{lacW}XpdSH2137
Cytology
Description

Insertion 7bp upstream of the 3' splice junction of the first intron.

(Li et al., 2010)
Allele components
Component
Use(s)
Inserted element
P{lacW}
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class

      abnormal mitotic cell cycle | embryonic cycle 10, with XpdG47R

      (Stettler et al., 2015)

      abnormal mitotic cell cycle | embryonic cycle 10, with XpdG675R

      (Stettler et al., 2015)

      abnormal mitotic cell cycle | embryonic cycle 10, with XpdR112H

      (Stettler et al., 2015)

      abnormal mitotic cell cycle | embryonic cycle 10, with XpdR601L

      (Stettler et al., 2015)

      abnormal mitotic cell cycle | embryonic cycle 10, with XpdR683W

      (Stettler et al., 2015)

      abnormal mitotic cell cycle | embryonic cycle 10, with XpdR722W

      (Stettler et al., 2015)

      abnormal mitotic cell cycle | embryonic stage

      (Stettler et al., 2015)

      abnormal mitotic cell cycle | embryonic stage | maternal effect, with Scer\GAL4αTub84B.PL, XpdUAS.Tag:V5

      (Li et al., 2010)

      lethal

      (Stettler et al., 2015)

      lethal, with XpdG602D

      (Stettler et al., 2015)

      lethal, with XpdR616P

      (Stettler et al., 2015)

      lethal, with XpdR616W

      (Stettler et al., 2015)

      lethal, with XpdR658H

      (Stettler et al., 2015)

      lethal, with XpdR683Q

      (Stettler et al., 2015)

      lethal (with Df(2R)K11)

      (Li et al., 2010)

      lethal | recessive

      (Li et al., 2010, Oh et al., 2003)

      lethal - all die during embryonic stage | maternal effect, with Scer\GAL4αTub84B.PL, XpdUAS.Tag:V5

      (Li et al., 2010)

      partially lethal - majority die | conditional, with XpdD234N

      (Stettler et al., 2015)

      partially lethal - majority die | conditional, with XpdG47R

      (Stettler et al., 2015)

      partially lethal - majority die | conditional, with XpdG675R

      (Stettler et al., 2015)

      radiation sensitive | embryonic stage, with XpdD234N

      (Stettler et al., 2015)

      radiation sensitive | embryonic stage, with XpdG47R

      (Stettler et al., 2015)

      radiation sensitive | embryonic stage, with XpdG675R

      (Stettler et al., 2015)

      some die during embryonic stage | maternal effect, with Scer\GAL4αTub84B.PL, XpdUAS.Tag:V5

      (Li et al., 2010)
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      In comparison with wild-type, transgenic XpdD234N embryos in a XpdSH2137 mutant genetic background show highly enhanced death rates after ultraviolet light irradiation with a dose of 100 J/m[2].

      In comparison with wild-type, transgenic XpdG47R embryos in a XpdSH2137 mutant genetic background show highly enhanced death rates after ultraviolet light irradiation with a dose of 100 J/m[2].

      In comparison with wild-type, transgenic XpdG675R embryos in a XpdSH2137 mutant genetic background show highly enhanced death rates after ultraviolet light irradiation with a dose of 100 J/m[2].

      Compared with wild-type, elevated cell-cycle synchrony defects are detected in transgenic XpdR601L embryos in a XpdSH2137 mutant genetic background.

      Compared with wild-type, elevated cell-cycle synchrony defects are detected in transgenic XpdR683W embryos in a XpdSH2137 mutant genetic background.

      Compared with wild-type, elevated cell-cycle synchrony defects are detected in transgenic XpdG47R embryos in a XpdSH2137 mutant genetic background.

      Compared with wild-type, elevated cell-cycle synchrony defects are detected in transgenic XpdG675R embryos in a XpdSH2137 mutant genetic background.

      Compared with wild-type, elevated cell-cycle synchrony defects are detected in transgenic XpdR112H embryos in a XpdSH2137 mutant genetic background.

      Compared with wild-type, elevated cell-cycle synchrony defects are detected in transgenic XpdR722W embryos in a XpdSH2137 mutant genetic background.

      Compared with wild-type, an enhanced rate of DNA loss and abnormal free centrosomes that often continue to divide in subsequent cycles is detected in transgenic XpdR683W embryos in a XpdSH2137 genetic background.

      Compared with wild-type, an enhanced rate of DNA loss and abnormal free centrosomes that often continue to divide in subsequent cycles is detected in transgenic XpdG47R embryos in a XpdSH2137 genetic background.

      Compared with wild-type, an enhanced rate of DNA loss and abnormal free centrosomes that often continue to divide in subsequent cycles is detected in transgenic XpdG675R embryos in a XpdSH2137 genetic background.

      Compared with wild-type, an enhanced rate of DNA loss and abnormal free centrosomes that often continue to divide in subsequent cycles is detected in transgenic XpdR112H embryos in a XpdSH2137 genetic background.

      (Stettler et al., 2015)

      Embryos derived from homozygous XpdSH2137 females expressing XpdScer\UAS.T:SV5\V5 under the control of Scer\GAL4αTub84B.PL do not hatch into larvae. 38.7% of the embryos show nuclear division defects during the nuclear division cycles. The nuclear division defects can be restricted to a small part of the embryo or may affect larger regions. The defective nuclei fail to segregate their chromatin properly and show nuclear fusions and chromatin bridges (these can be seen in interphase, late anaphase or telophase). Mitotic figures are seen in which chromosome segregation has failed to occur properly by telophase. Some of the mutant embryos have chromatin-free regions containing free centrosomes (suggesting that improperly divided nuclei have fallen into the interior of the embryo during previous cycles). Some of these extra centrosomes are seen associated with nearby nuclei. The synchronization of late syncytial division cycles is also often lost in these embryos.

      Nuclear cycles 12 and 13 usually take much longer than normal in embryos derived from homozygous XpdSH2137 females expressing XpdScer\UAS.T:SV5\V5 under the control of Scer\GAL4αTub84B.PL. Delays of 50-100% are often seen at the anterior end of the embryo, while the delays at the posterior end can be much longer. The mitotic wave from the anterior pole often proceeds almost or entirely to the posterior end before the posterior mitotic wave is initiated (in contrast to wild type).

      Embryos derived from homozygous XpdSH2137 females expressing XpdScer\UAS.T:SV5\V5 under the control of Scer\GAL4αTub84B.PL have a highly elevated frequency of mitotic spindles that branch out into neighbouring mitotic figures, with microtubules from one spindle "reaching over" to the neighbouring mitotic figure and appearing to attach to a chromosome.

      (Li et al., 2010)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      XpdSH2137/Xpd[+] is a suppressor | partially of eye phenotype of Scer\GAL4GMR.PU, crbintra.UAS.Tag:MYC

      (Yeom et al., 2015)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      One copy of XpdSH2137 strongly suppresses the small and rough eye phenotype seen when crbintra.Scer\UAS.T:Hsap\MYC is expressed under the control of Scer\GAL4GMR.PU.

      (Yeom et al., 2015)

      The nuclear division defects seen in embryos derived from homozygous XpdSH2137 females expressing XpdScer\UAS.T:SV5\V5 under the control of Scer\GAL4αTub84B.PL are partially rescued if the females are carrying either CycB2/+ or Df(2R)59AB/+.

      (Li et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      XpdSH2137 is rescued by Xpd+t.wt

      (Stettler et al., 2015)

      XpdSH2137 is rescued by XpdS541R

      (Stettler et al., 2015)

      XpdSH2137 is rescued by XpdY542C

      (Stettler et al., 2015)

      XpdSH2137 is rescued by XpdR683W

      (Stettler et al., 2015)

      XpdSH2137 is rescued by XpdR658C

      (Stettler et al., 2015)

      XpdSH2137/Df(2R)K11 is rescued by Xpd+t5.9

      (Li et al., 2010)

      XpdSH2137 is rescued by Xpd+t5.9

      (Li et al., 2010)
      Partially rescued by

      XpdSH2137 is partially rescued by XpdD234N

      (Stettler et al., 2015)

      XpdSH2137 is partially rescued by XpdR601L

      (Stettler et al., 2015)

      XpdSH2137 is partially rescued by XpdG47R

      (Stettler et al., 2015)

      XpdSH2137 is partially rescued by XpdG675R

      (Stettler et al., 2015)

      XpdSH2137 is partially rescued by XpdR112H

      (Stettler et al., 2015)

      XpdSH2137 is partially rescued by XpdR722W

      (Stettler et al., 2015)

      XpdSH2137/Df(2R)K11 is partially rescued by XpdTag:V5

      (Li et al., 2010)

      XpdSH2137 is partially rescued by XpdUAS.Tag:V5/Scer\GAL4αTub84B.PL

      (Li et al., 2010)
      Not rescued by

      XpdSH2137 is not rescued by XpdR616P

      (Stettler et al., 2015)

      XpdSH2137 is not rescued by XpdR616W

      (Stettler et al., 2015)

      XpdSH2137 is not rescued by XpdR683Q

      (Stettler et al., 2015)

      XpdSH2137 is not rescued by XpdG602D

      (Stettler et al., 2015)

      XpdSH2137 is not rescued by XpdR658H

      (Stettler et al., 2015)

      XpdSH2137/Df(2R)K11 is not rescued by XpdGFP

      (Li et al., 2010)

      XpdSH2137/Df(2R)K11 is not rescued by XpdYFP

      (Li et al., 2010)
      Comments

      Xpd+t.wt rescues the lethality of XpdSH2137. Xpd+t.wt also rescues the cell-cycle synchronisation defects in XpdSH2137 mutant embryos.

      (Stettler et al., 2015)

      XpdT:SV5\V5 rescues between 1/3 and 1/5 of the expected number of XpdSH2137/Df(2R)K11 flies.

      XpdSH2137 animals expressing XpdScer\UAS.T:SV5\V5 under the control of Scer\GAL4αTub84B.PL develop to the adult stage. The rescued adults do not show any morphological abnormalities and rescued males are fertile. Rescued females show normal egg production, however, while the resulting embryos initiate developmental normally, no larvae emerge from them.

      (Li et al., 2010)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      XpdSH2137
      Xpdp
      (Jung et al., 2020)
      l(2)SH2137SH2137
      xpdP
      (Stettler et al., 2015, Yeom et al., 2015, Li et al., 2010)
      Name Synonyms
      Secondary FlyBase IDs
        References (6)