FlyBase Allele Report: Dmel\Rbf2[UAS.cSa]

General Information

Symbol

Dmel\Rbf2^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Stevaux

FlyBase ID

FBal0144644

Feature type

allele

Associated gene

Dmel\Rbf2

Associated Insertion(s)

Carried in Construct

P{UAS-Rbf2.S}

Key Links

Transgenic product class

cDNA

(Stevaux et al., 2002)

wild_type

(Stevaux et al., 2002)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Stevaux et al., 2002)

wild_type

(Stevaux et al., 2002)

Mutagen

in vitro construct

(Stevaux et al., 2002)

Progenitor genotype

Carried in construct

P{UAS-Rbf2.S}

Cytology

Description

UASt sequences drive expression of a full-length Rbf2 cDNA.

(Stevaux et al., 2002)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Rbf2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

When Rbf2^Scer\UAS.cSa is driven by Scer\GAL4^ap-md544 no visible wing phenotype is seen. When Rbf2^Scer\UAS.cSa is driven by Scer\GAL4^ey.PH no visible eye phenotype is seen.

(Stevaux et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

E2f2^UAS.cFa, Rbf2^UAS.cSa, Scer\GAL4^ap-md544 has abnormal size | larval stage phenotype

(Stevaux et al., 2002)

E2f2^UAS.cFa, Rbf2^UAS.cSa, Scer\GAL4^ap-md544 has abnormal size | adult stage phenotype

(Stevaux et al., 2002)

E2f2^UAS.cFa, Rbf2^UAS.cSa, Scer\GAL4^ap-md544 has decreased occurrence of cell division | larval stage phenotype

(Stevaux et al., 2002)

Phenotype Manifest In

Other

Statement

Reference

E2f2^UAS.cFa, Rbf2^UAS.cSa, Scer\GAL4^ey.PH has eye phenotype

(Stevaux et al., 2002)

E2f2^UAS.cFa, Rbf2^UAS.cSa, Scer\GAL4^ey.PH has wing disc phenotype

(Stevaux et al., 2002)

E2f2^UAS.cFa, Rbf2^UAS.cSa, Scer\GAL4^ey.PH has S phase & dorsal mesothoracic disc phenotype

(Stevaux et al., 2002)

E2f2^UAS.cFa, Rbf2^UAS.cSa, Scer\GAL4^ap-md544 has wing phenotype

(Stevaux et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The combination of E2f2^Scer\UAS.cFa and Rbf2^Scer\UAS.cSa driven by Scer\GAL4^ap-md544 leads to a clearly visible wing atrophy phenotype. Wing discs from mutant larvae have phenotypes that vary from being shrunken and abnormal to those that have a relative normal morphology but have many cells that show a decrease in DNA synthesis and have a much higher of proportion of cells in G1 phase, and a lower proportion in S and G2 phases. The combination of E2f2^Scer\UAS.cFa and Rbf2^Scer\UAS.cSa driven by Scer\GAL4^ey.PH leads to a clearly visible small eye phenotype.

(Stevaux et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Rbf2^Scer\UAS.cSa

Rbf2^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Stevaux

(Stevaux et al., 2002)

Secondary FlyBase IDs

References (2)

Report Sections

Open Close